Scutellarin Alleviates Neuronal Apoptosis in Ischemic Stroke via Activation of the PI3K/AKT Signaling Pathway
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(5), С. 2175 - 2175
Опубликована: Фев. 28, 2025
Among
all
stroke
types,
ischemic
(IS)
occurs
most
frequently,
resulting
in
neuronal
death
and
tissue
injury
within
both
the
central
infarct
region
surrounding
areas.
This
study
explored
neuroprotective
mechanisms
of
scutellarin,
a
flavonoid
compound,
through
an
integrated
strategy
that
merged
silico
analyses
(including
network
pharmacology
molecular
docking
simulations)
with
vitro
vivo
experimental
verification.
We
identified
1887
IS-related
targets
129
scutellarin
targets,
23
overlapping
targets.
PPI
analysis
revealed
five
core
demonstrated
strong
binding
affinities
between
these
Bioinformatic
analyses,
including
GO
functional
annotation
KEGG
pathway
mapping,
indicated
PI3K/AKT
cascade
represents
primary
signaling
mechanism.
An
system
was
developed
using
PC12
cells
under
oxygen-glucose
deprivation
conditions
to
investigate
how
regulates
cell
via
pathway.
Western
blot
quantification
treatment
enhanced
expression
p-PI3K,
p-AKT,
Bcl-2
proteins,
while
simultaneously
reducing
levels
apoptotic
markers
Bax
cleaved
caspase-3.
Furthermore,
pharmacological
intervention
selective
PI3K
inhibitor
LY294002
attenuated
alterations,
diminished
Bcl-2,
accompanied
by
elevated
In
rat
model
middle
cerebral
artery
occlusion,
administration
comparable
effects,
maintaining
survival
modulating
protein
activation.
Collectively,
this
demonstrates
therapeutic
potential
ischemia
modulation,
suggesting
its
possible
application
treating
disorders.
Язык: Английский
Network pharmacology and experimental verification to explore the molecular mechanisms of Astragaloside IV against diabetic encephalopathy
Biochemical and Biophysical Research Communications,
Год журнала:
2025,
Номер
unknown, С. 151778 - 151778
Опубликована: Апрель 1, 2025
Язык: Английский
Epidermal growth factor receptor pathway in central nervous system regenerative medicine: a narrative review
Regenerative medicine reports .,
Год журнала:
2024,
Номер
1(2), С. 172 - 186
Опубликована: Дек. 1, 2024
The
epidermal
growth
factor
receptor
(signaling
pathway
is
involved
in
numerous
important
mammal
cell
functions,
such
as
growth,
survival,
proliferation,
and
differentiation.
Associated
with
this,
it
tissue
normal
development
tumorigenesis,
repair.
Epidermal
broadly
expressed
the
organism,
including
central
peripheral
nervous
system,
where
has
neurotrophic
functions.
system
a
limited
capacity
for
regeneration
upon
lesion,
leading
to
severe
disabilities,
blindness,
sensorimotor
deficits,
paralysis.
However,
observations
suggest
that
its
innate
neurogenic
response
may
be
amplified
promote
replenishment
of
damaged
neuronal
populations.
In
addition,
environment
can
also
modulated
regeneration.
Therefore,
pathways
neural
repair
have
been
investigated
terms
regenerative
medicine,
discover
new
therapies
recover
from
traumatic
injury
or
ischemia
inhibit
progression
neurodegenerative
diseases.
Growth
factors
widely
accepted
most
promoters
proliferation
neurogenesis
under
physiologic
conditions
after
injury.
this
review,
we
summarize
how
signaling
damage
neurodegeneration
several
settings,
olfactory
epithelium,
brain,
spinal
cord,
optic
nerve,
context
medicine
and,
therefore,
neurological
function
reestablishment
insults.
Язык: Английский