Correlation of fecal microbiome dysregulation to synovial transcriptome in an equine model of obesity associated osteoarthritis DOI Open Access
Lyndah Chow,

Gabriella Kawahisa-Piquini,

Luke Bass

и другие.

Annals of Translational Medicine, Год журнала: 2024, Номер 12(6), С. 112 - 112

Опубликована: Дек. 1, 2024

Osteoarthritis (OA) is increasingly thought to be a multifactorial disease in which sustained gut inflammation serves as continued source of inflammatory mediators driving degenerative processes at distant sites such joints. The objective this study was use the equine model naturally occurring obesity associated OA compare fecal microbiome and health correlate those findings differential gene expression synovial fluid (SF) cells, circulating leukocytes cytokine levels (plasma, SF) towards improved understanding interplay between immune transcriptome pathophysiology. Feces, peripheral blood mononuclear cells (PBMCs), SF were isolated from healthy skeletally mature horses (n=12; 6 males, females) with (n=6, 2 females, 4 males). Horses determined have via lameness evaluation, response intra-articular (IA) diagnostic analgesia, radiographic arthroscopic evidence. excluded who had received medications or joint injections within months. Cytokine analyses plasma performed multiplex immunoassay. Fecal bacterial microbial 16s DNA sequencing correlated bulk RNA PBMC using an Illumina based platform. higher body condition scores (P=0.009). Cytokines elevated [interleukin (IL)-2, IL-6, IL-18, interferon gamma (IFN-γ), inducible protein 10 (CXCL10 IP-10), granulocyte colony-stimulating factor (G-CSF)] (IL-1β, IL-17A, IP-10, G-CSF) OA. Microbial principal coordinate analysis (PCoA) Bray-Curtis dissimilarity for β-diversity demonstrated distinct grouping samples versus (P=0.003). Faith alpha diversity reduced (P=0.02). Analysis composition showed relative abundance taxa on multiple Specific phyla (Firmicutes, Verrucomicrobia, Tenericutes, Fibrobacteres), transcriptomic differences related cell structure, extracellular matrix, collagen, laminin, migration, motility, These provide compelling evidence link obesity, dysbiosis development relevant large animal model, establishing connection here that provides platform therapeutic interventions targeting can build.

Язык: Английский

The Gut Microbiome and Joint Microbiome Show Alterations in Patients With Knee Osteoarthritis Versus Controls: A Systematic Review DOI
Ron Gilat, Allen A. Yazdi, Alexander C. Weissman

и другие.

Arthroscopy The Journal of Arthroscopic and Related Surgery, Год журнала: 2024, Номер unknown

Опубликована: Май 1, 2024

Язык: Английский

Процитировано

4

The Gut Feeling: The Role of Gut Microbiome in Orthopedics DOI Creative Commons
Janki Sharan Bhadani, Vikas Agashe, Ashok Shyam

и другие.

Journal of Orthopaedic Case Reports, Год журнала: 2025, Номер 15(3), С. 308 - 311

Опубликована: Янв. 1, 2025

Introduction: Advancements in microbiome-targeted therapies, including probiotics, prebiotics, and fecal microbial transplantation, offer exciting possibilities for orthopedic care. Probiotics, live beneficial microorganisms found fermented foods, such as yogurt, kefir, sauerkraut, kimchi, cheese, help maintain a healthy gut microbiome. Prebiotics fiber-rich foods onions, garlic, whole grains, nourish these bacteria, supporting their growth activity. Together, therapies regulate health, promote immune resilience, reduce infection risks, accelerate healing – key factors outcomes [1, 2]. The microbiome, diverse ecosystem of microorganisms, plays pivotal role maintaining overall health [3]. Beyond digestion, it influences regulation, inflammation control, musculoskeletal well-being [4]. Gut significantly impacts outcomes, bone healing, density. An imbalance this ecosystem, known dysbiosis, can compromise recovery increase risks [5]. Supporting through dietary modifications, or prebiotics holds the potential to enhance patient [6]. Orthopedic Infections Health: Periprosthetic joint infections fracture-related remain formidable challenges Biofilm formation on implants protect bacteria from antibiotics responses, complicating treatment [7]. Dysbiosis further exacerbate by allowing enter bloodstream colonize surgical sites [8]. Maintaining balanced microbiome body’s defenses, limit bacterial migration, implant longevity. Fracture Healing Microbial Influence: Bone repair is finely regulated process involving inflammation, new formation, remodeling [9]. A an essential managing moderating promoting cell Disruptions lead excessive hinder ability heal effectively [10]. may improve patients prone delayed complications. Autoimmune Conditions Increased Susceptibility Infection: diseases, rheumatoid arthritis, heighten susceptibility due weakened function [11]. worsen imbalance, increasing risk migration sites. Ensuring fortify resilience post-surgical complications with conditions [12]. Pain Management Microbiome Modulation: affects pain perception influencing production neurotransmitters that pathways. heightened sensitivity, posing chronic osteoarthritis [13]. Optimizing additional approach complement traditional management strategies dependency medications. Density Mineral Absorption critical absorbing minerals vital strength balance impair absorption process, contributing osteoporosis [14]. Emerging research suggests specific probiotics mineral absorption, providing supportive therapy improving reducing fracture [15]. Post-Surgical Recovery Systemic influence extends beyond local sites, playing systemic following surgery. supports faster reduced fewer post-operative Conversely, imbalances delay [16]. Strategies perioperative period rehabilitation. Innovative Approaches Care: Screening before surgery could allow timely interventions, minimizing outcomes. While some experimental, they represent promising avenues future developments personalized [17]. Recent has revealed alterations, particularly those affecting arginine metabolism, play significant structural [18]. Mechanical loading crucial but its effectiveness often hampered high variability mechanoreceptor activity influenced microbes. Studies have shown depletion profoundly responsiveness, indicating possible pathway therapeutic strategies. gut-bone axis, concept gaining attention, connects state [19]. This relationship opens up changes treat inflammatory arthritis. By understanding researchers are uncovering mechanisms offering hope more effective treatments future. To summarize roles practice, table highlights implications care (Table 1). Conclusion: microbiome’s infection, presents area exploration. For surgeons clinicians, offers preventive pathways, associated fractures. Future bring even transforming practices ways extend approaches. integrating into strategies, health-care providers foster stronger, resilient patients, advancing field toward holistic

Язык: Английский

Процитировано

0

The Impact of Microbiota on Musculoskeletal Injuries DOI Creative Commons

Giada La Placa,

Marcello Covino, Marcello Candelli

и другие.

Cells, Год журнала: 2025, Номер 14(7), С. 554 - 554

Опубликована: Апрель 7, 2025

Musculoskeletal injuries comprise a wide range of physical conditions impacting the coordination bones, muscles, and joints. Estimations suggest that close to one-third world’s population will experience musculoskeletal or non-musculoskeletal injury at some point in their life. affect athletes, office workers, industrial older adults, children every year. Among individuals over age 65, disproportionately women, limiting ability maintain an active professional life engage leisure activities during retirement. The field therapy has recently expanded build understanding complex, non-linear interactions between gut microbiota system. There is unexpected connection both pain healing process following injuries. Understanding mechanisms microbiota’s influence on these could inform healthcare strategies aimed prevention recovery. For patients who suffer from are risk developing injuries, analyzing composition plays crucial role patient stratification, which can significantly enhance effectiveness treatment strategies.

Язык: Английский

Процитировано

0

Integrative review of the gut microbiome’s role in pain management for orthopaedic conditions DOI
Naveen Jeyaraman,

Madhan Jeyaraman,

Priya Dhanpal

и другие.

World Journal of Experimental Medicine, Год журнала: 2025, Номер 15(2)

Опубликована: Апрель 11, 2025

The gut microbiome, a complex ecosystem of microorganisms, has significant role in modulating pain, particularly within orthopaedic conditions. Its impact on immune and neurological functions is underscored by the gut-brain axis, which influences inflammation, pain perception, systemic responses. This integrative review examines current research how dysbiosis associated with various pathways, notably nociceptive neuroinflammatory mechanisms linked to central sensitization. We highlight advancements meta-omics technologies, such as metagenomics metaproteomics, deepen our understanding microbiome-host interactions their implications pain. Recent studies emphasize that gut-derived short-chain fatty acids microbial metabolites play roles neuroinflammation nociception, contributing management. Probiotics, prebiotics, synbiotics, faecal microbiome transplants are explored potential therapeutic strategies alleviate through modulation, offering an adjunct or alternative opioids. However, variability individual microbiomes poses challenges standardizing these treatments, necessitating further rigorous clinical trials. A multidisciplinary approach combining microbiology, immunology, neurology, orthopaedics essential develop innovative, personalized management rooted health, transform care.

Язык: Английский

Процитировано

0

Mapping knowledge landscapes and research frontiers of gastrointestinal microbiota and bone metabolism: a text-mining study DOI Creative Commons
Haiyang Wu,

Zaijie Sun,

Qiang Guo

и другие.

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Июль 10, 2024

Extensive research efforts have been dedicated to elucidating the intricate pathways by which gastrointestinal microbiota and their metabolites exert influence on processes of bone formation. Nonetheless, a notable gap exists in literature concerning bibliometric analysis trends at nexus metabolism.

Язык: Английский

Процитировано

1

Metagenomic changes in response to antibiotic treatment in severe orthopaedic trauma patients DOI Creative Commons
Afroditi Kouraki, Amy Zheng, Stephanie Miller

и другие.

iScience, Год журнала: 2024, Номер 27(9), С. 110783 - 110783

Опубликована: Авг. 23, 2024

We investigated changes in microbiome composition and abundance of antimicrobial resistance (AMR) genes post-antibiotic treatment severe trauma patients. Shotgun sequencing revealed beta diversity (Bray-Curtis) differences between 16 hospitalized multiple rib fractures patients 10 age- sex-matched controls (

Язык: Английский

Процитировано

1

Correlation of fecal microbiome dysregulation to synovial transcriptome in an equine model of obesity associated osteoarthritis DOI Open Access
Lyndah Chow,

Gabriella Kawahisa-Piquini,

Luke Bass

и другие.

Annals of Translational Medicine, Год журнала: 2024, Номер 12(6), С. 112 - 112

Опубликована: Дек. 1, 2024

Osteoarthritis (OA) is increasingly thought to be a multifactorial disease in which sustained gut inflammation serves as continued source of inflammatory mediators driving degenerative processes at distant sites such joints. The objective this study was use the equine model naturally occurring obesity associated OA compare fecal microbiome and health correlate those findings differential gene expression synovial fluid (SF) cells, circulating leukocytes cytokine levels (plasma, SF) towards improved understanding interplay between immune transcriptome pathophysiology. Feces, peripheral blood mononuclear cells (PBMCs), SF were isolated from healthy skeletally mature horses (n=12; 6 males, females) with (n=6, 2 females, 4 males). Horses determined have via lameness evaluation, response intra-articular (IA) diagnostic analgesia, radiographic arthroscopic evidence. excluded who had received medications or joint injections within months. Cytokine analyses plasma performed multiplex immunoassay. Fecal bacterial microbial 16s DNA sequencing correlated bulk RNA PBMC using an Illumina based platform. higher body condition scores (P=0.009). Cytokines elevated [interleukin (IL)-2, IL-6, IL-18, interferon gamma (IFN-γ), inducible protein 10 (CXCL10 IP-10), granulocyte colony-stimulating factor (G-CSF)] (IL-1β, IL-17A, IP-10, G-CSF) OA. Microbial principal coordinate analysis (PCoA) Bray-Curtis dissimilarity for β-diversity demonstrated distinct grouping samples versus (P=0.003). Faith alpha diversity reduced (P=0.02). Analysis composition showed relative abundance taxa on multiple Specific phyla (Firmicutes, Verrucomicrobia, Tenericutes, Fibrobacteres), transcriptomic differences related cell structure, extracellular matrix, collagen, laminin, migration, motility, These provide compelling evidence link obesity, dysbiosis development relevant large animal model, establishing connection here that provides platform therapeutic interventions targeting can build.

Язык: Английский

Процитировано

0