The Influence of Flavonoid Compositions on Nrf2 Transcription Factor Expression in Case of Infections Triggered by Influenza A Virus and Transmissible Gastroenteritis Coronavirus DOI Creative Commons
Maryna Arkhypova, О. М. Дерябін, T. Yu. Trokhymchuk

и другие.

Innovative Biosystems and Bioengineering, Год журнала: 2023, Номер 7(4), С. 48 - 56

Опубликована: Дек. 15, 2023

Background. Viral infections trigger transcription factors, including Nrf2, which regulate the expression of genes related to cytokines, chemokines, and more. Nuclear factor erythroid 2-related 2 (Nrf2) participates in complex regulatory networks controlling cytoprotective immune responses. Recently, new roles have been attributed regulation antiviral Objective. The aim study was analyze Nrf2 activation influenza virus infection vivo transmissible gastroenteritis coronavirus (TGEV) vitro, as well effects flavonoid compositions Proteflazidum® Protoil on these two experimental models viral infection. Methods. Outbred white mice were infected with A (A/FM/1/47/H1N1 strain). Jurkat cells TGEV, previously adapted cells. Flavonoid (Ecopharm, Ukraine), containing mixture tricin, luteolin, apigenin, quercetin, rhamnosin, used corresponding dilutions. Real-Time PCR employed RNA lungs both uninfected virus-infected Additionally, ELISA assess peptide. Results. showed a tendency increase within 100-fold range. In treated or Protoil, level decreased about 10-fold compared untreated mice. TGEV resulted Both TGEV-infected cells, while their intact Day 1 not detected. Conclusions. only slight effect However, case infection, they counteract extensive up-regulation due

Язык: Английский

The Influence of Flavonoid Compositions on Nrf2 Transcription Factor Expression in Case of Infections Triggered by Influenza A Virus and Transmissible Gastroenteritis Coronavirus DOI Creative Commons
Maryna Arkhypova, О. М. Дерябін, T. Yu. Trokhymchuk

и другие.

Innovative Biosystems and Bioengineering, Год журнала: 2023, Номер 7(4), С. 48 - 56

Опубликована: Дек. 15, 2023

Background. Viral infections trigger transcription factors, including Nrf2, which regulate the expression of genes related to cytokines, chemokines, and more. Nuclear factor erythroid 2-related 2 (Nrf2) participates in complex regulatory networks controlling cytoprotective immune responses. Recently, new roles have been attributed regulation antiviral Objective. The aim study was analyze Nrf2 activation influenza virus infection vivo transmissible gastroenteritis coronavirus (TGEV) vitro, as well effects flavonoid compositions Proteflazidum® Protoil on these two experimental models viral infection. Methods. Outbred white mice were infected with A (A/FM/1/47/H1N1 strain). Jurkat cells TGEV, previously adapted cells. Flavonoid (Ecopharm, Ukraine), containing mixture tricin, luteolin, apigenin, quercetin, rhamnosin, used corresponding dilutions. Real-Time PCR employed RNA lungs both uninfected virus-infected Additionally, ELISA assess peptide. Results. showed a tendency increase within 100-fold range. In treated or Protoil, level decreased about 10-fold compared untreated mice. TGEV resulted Both TGEV-infected cells, while their intact Day 1 not detected. Conclusions. only slight effect However, case infection, they counteract extensive up-regulation due

Язык: Английский

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