Unraveling Immune-Related lncRNAs in Breast Cancer Molecular Subtypes DOI Creative Commons
Carolina Mathias, João C. D. Muzzi,

Bruna Borba Antunes

и другие.

Frontiers in Oncology, Год журнала: 2021, Номер 11

Опубликована: Май 31, 2021

Breast cancer (BRCA) is the most leading cause of worldwide. It a heterogeneous disease with at least five molecular subtypes including luminal A, B, basal-like, HER2-enriched, and normal-like. These are usually stratified according to their mRNA profile patterns; however, ncRNAs increasingly being used for this purpose. Among class, long non-coding RNAs (lncRNAs) molecules more than 200 nucleotides versatile regulatory roles; high tissue-specific expression profiles. The heterogeneity BRCA can also be reflected regarding tumor microenvironment immune cells composition, which directly impact patient’s prognosis therapy response. Using immunogenomics data from previous study, we propose here bioinformatics approach include lncRNAs complexity in subtype. RNA-seq Cancer Genome Atlas (TCGA) cohort was analyzed, signal-to-noise ratio metrics were applied create these subtype-specific signatures. Five immune-related signatures generated approximately ten specific lncRNAs, then functionally analyzed using GSEA enrichment survival analysis. We highlighted some each LINC01871 related response activation favorable overall basal-like samples; EBLN3P suppression progression MEG3, XXYLT1-AS2, LINC02613 HER2-enriched normal-like subtypes, respectively. In way, emphasize need know better role as regulators provide new perspectives diagnosis, therapeutical targets subtypes.

Язык: Английский

Various LncRNA Mechanisms in Gene Regulation Involving miRNAs or RNA-Binding Proteins in Non-Small-Cell Lung Cancer: Main Signaling Pathways and Networks DOI Open Access
Э. А. Брага, М. В. Фридман, A. M. Burdennyy

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(17), С. 13617 - 13617

Опубликована: Сен. 3, 2023

Long non-coding RNAs (lncRNAs) are crucial players in the pathogenesis of non-small-cell lung cancer (NSCLC). A competing binding lncRNAs and mRNAs with microRNAs (miRNAs) is one most common mechanisms gene regulation by NSCLC, which has been extensively researched last two decades. However, alternative that do not depend on miRNAs have also reported. Among them, intriguing mechanism mediated RNA-binding proteins (RBPs) such as IGF2BP1/2/3, YTHDF1, HuR, FBL, increase stability target mRNAs. IGF2BP2 YTHDF1 may be involved m6A modification or Some lncRNAs, DLGAP1-AS2, MALAT1, MNX1-AS1, SNHG12, several depending target: lncRNA/miRNA/mRNA interactome through RBP. The protein sets selected here were then analyzed using DAVID database to identify pathways overrepresented KEGG, Wikipathways, Reactome pathway. Using STRING website, we assessed interactions between built networks. Our analysis revealed JAK-STAT Hippo signaling pathways, cytokine VEGFA-VEGFR2 pathway, cell cycle regulation, neovascularization relevant effect lncRNA NSCLC.

Язык: Английский

Процитировано

24
Long Non-Coding RNA: Dual Effects on Breast Cancer Metastasis and Clinical Applications DOI Open Access
Q. Huang, Guofeng Liu, Xianling Qian

и другие.

Cancers, Год журнала: 2019, Номер 11(11), С. 1802 - 1802

Опубликована: Ноя. 16, 2019

As a highly heterogeneous malignancy, breast cancer (BC) has become the most significant threat to female health. Distant metastasis and therapy resistance of BC are responsible for cases mortality recurrence. relies on an array processes, such as cell proliferation, epithelial-to-mesenchymal transition (EMT), mesenchymal-to-epithelial (MET), angiogenesis. Long non-coding RNA (lncRNA) refers class with length over 200 nucleotides. Currently, rising number studies have managed investigate association between lncRNA. In this study, we summarized how lncRNA dual effects in by regulating invasion, migration, distant cells. We also emphasize that crucial regulatory stemness angiogenesis BC. Clinically, some lncRNAs can regulate chemotherapy sensitivity patients may function novel biomarkers diagnose or predict prognosis patients. The exact impact clinical relevance deserves further study. This review be approach understanding BC, thereby linking quasi-personalized treatment future.

Язык: Английский

Процитировано

59
Exosomes in triple negative breast cancer: Garbage disposals or Trojan horses? DOI Creative Commons
Chia Yin Goh, Cathy Wyse, Matthew Ho

и другие.

Cancer Letters, Год журнала: 2020, Номер 473, С. 90 - 97

Опубликована: Янв. 2, 2020

Triple negative breast cancer (TNBC) is a subtype which particularly aggressive and invasive. The treatment of TNBC has been limited due to the lack well-defined molecular targets. Exosomes are nano-sized extracellular vesicles that released from virtually all cell types into space. Due their endocytic origin, exosomes carry valuable information cells origin. were first thought serve as "garbage disposals" eliminate unwanted cellular components. Later, they found be involved in pathology many diseases including cancer. Despite established roles multiple diseases, only small number studies have focused on role TNBC. In this review, we outline progression, metastasis drug resistance subtype. We then further illustrate potential diagnostic tools, therapeutic targets delivery systems.

Язык: Английский

Процитировано

56
LncRNA MEG3 promotes melanoma growth, metastasis and formation through modulating miR-21/E-cadherin axis DOI Creative Commons
Liangcai Wu, Lifei Zhu, Yanchang Li

и другие.

Cancer Cell International, Год журнала: 2020, Номер 20(1)

Опубликована: Янв. 10, 2020

Melanoma is the most aggressive type of skin cancer with high mortality rate and poor prognosis. lncRNA MEG3, a tumor suppressor, closely related to development various cancers. However, role MEG3 in melanoma has seldom been studied.RT-PCR was used examine expressions E-cadherin patients cell lines. Then, biological functions were demonstrated by transfecting MEG3-siRNA, MEG3-overexpression, E-cadherin-siRNA E-cadherin-overexpression plasmids Moreover, CCK8 assay colony formation utilized assess proliferation; Transwell performed evaluate invasive ability; xenografts nude mice applied test generation. Additionally, target interactions among miR-21 determined dual luciferase reporter assay. Finally, RT-PCR WB further conducted verify regulatory roles E-cadherin.The clinical data showed that both declined carcinoma tissues as compared their para-carcinoma tissues. B16 cells also higher than those A375 A2058 cells. Subsequently, based on differently expressed these human lines, we chose B16, for following experiments. The results could suppress growth, metastasis formation; meanwhile had same effects formation. Furthermore, analysis Kaplan-Meier curves confirmed there positive correlation between E-cadherin. Ultimately, assays directly which turn. revealed knockdown inhibited expression promoted expression, but overexpression presented completely opposite results.Our findings indicated might inhibit modulating miR-21/E-cadherin axis.

Язык: Английский

Процитировано

55
Deregulated expression of the imprinted DLK1-DIO3 region in glioblastoma stemlike cells: tumor suppressor role of lncRNA MEG3 DOI Creative Commons
Mariachiara Buccarelli, Valentina Lulli, Alessandro Giuliani

и другие.

Neuro-Oncology, Год журнала: 2020, Номер 22(12), С. 1771 - 1784

Опубликована: Май 21, 2020

Glioblastoma (GBM) stemlike cells (GSCs) are thought to be responsible for the maintenance and aggressiveness of GBM, most common primary brain tumor in adults. This study aims at elucidating involvement deregulations within imprinted delta-like homolog 1 gene‒type III iodothyronine deiodinase gene (DLK-DIO3) region on chromosome 14q32 GBM pathogenesis.Real-time PCR analyses were performed GSCs tissues. Methylation analyses, expression, reverse-phase protein array profiles used investigate suppressor function maternally expressed 3 (MEG3).Loss expression genes noncoding RNAs DLK1-DIO3 was observed tissues compared with normal brain. downregulation is mainly mediated by epigenetic silencing. Kaplan-Meier analysis indicated that low MEG3 MEG8 long (lnc)RNAs significantly correlated short survival patients. restoration impairs tumorigenic abilities vitro inhibiting cell growth, migration, colony formation decreases vivo reducing infiltrative growth. These effects associated modulation involved adhesion epithelial-to-mesenchymal transition (EMT).In acts as a regulating adhesion, EMT, proliferation, thus providing potential candidate novel therapies.

Язык: Английский

Процитировано

52
Non-coding RNAs related to angiogenesis in gynecological cancer DOI
Neda Rahimian, Zahra Razavi,

Fatemeh Aslanbeigi

и другие.

Gynecologic Oncology, Год журнала: 2021, Номер 161(3), С. 896 - 912

Опубликована: Март 27, 2021

Язык: Английский

Процитировано

45
Long Non-Coding RNAs in Diagnosis, Treatment, Prognosis, and Progression of Glioma: A State-of-the-Art Review DOI Creative Commons
Sara Momtazmanesh, Nima Rezaei

Frontiers in Oncology, Год журнала: 2021, Номер 11

Опубликована: Июль 12, 2021

Glioma is the most common malignant central nervous system tumor with significant mortality and morbidity. Despite considerable advances, exact molecular pathways involved in progression are not fully elucidated, patients commonly face a poor prognosis. Long non-coding RNAs (lncRNAs) have recently drawn extra attention for their potential roles different types of cancer as well non-malignant diseases. More than 200 lncRNAs been reported to be associated glioma. We aimed assess investigated stages mediating addition clinical applications. formation, invasion, progression, including regulating cell cycle, apoptosis, autophagy, epithelial-to-mesenchymal transition, stemness, angiogenesis, integrity blood-tumor-brain barrier, metabolism, immunological responses. The well-known oncogenic lncRNAs, which upregulated glioma, H19 , HOTAIR PVT1 UCA1 XIST CRNDE FOXD2-AS1 ANRIL HOXA11-AS TP73-AS1 DANCR . On other hand, MEG3 GAS5 CCASC2 TUSC7 suppressor downregulated. While studies effects MALAT1 TUG1 NEAT1 there some controversies regarding these lncRNAs. Expression levels can grade, survival, treatment response (chemotherapy drugs or radiotherapy), overall Moreover, circulatory such MALAT1, H19, HOTAIR, NEAT1, TUG1, GAS5, LINK-A provide non-invasive diagnostic prognostic tools. Modulation expression using antisense oligonucleotides lead novel therapeutics. Notably, profound understanding underlying function required develop therapeutic targets. investigations large sample sizes increased focus on in-vivo models expand our application

Язык: Английский

Процитировано

45
Long noncoding RNA Meg3 sponges miR-708 to inhibit intestinal tumorigenesis via SOCS3-repressed cancer stem cells growth DOI Creative Commons
Shuo Zhang, Weiwei Ji, Wei Wei

и другие.

Cell Death and Disease, Год журнала: 2021, Номер 13(1)

Опубликована: Дек. 21, 2021

Abstract Background Colorectal cancer (CRC) remains the most common gastrointestinal and a leading cause of deaths worldwide, with showing pathologies indicating malignant transformation early stage intestinal stem cells. The long non-coding RNA Meg3 , which functions as tumor suppressor, has been reported to be abnormal in multiple tumorigenesis events; however, underlying mechanism by contributes proliferation colonic cells unclear. Methods We analyzed expression levels miR-708 SOCS3 samples from Apc loss-of-function ( min ) mice patients CRC, particularly crypt AMO/DSS-induced model (in vivo) organoid culture system vitro) were used explore effect / /SOCS3 axis on colon. In vitro, we performed RNApull-down, immunoprecipitation, luciferase reporter assays using DLD1 RKO cell lines. Findings signaling plays critical role CRC development. Our data showed negatively correlate both clinical mouse model, indicated that acts competitive endogenous (ceRNA) . Then, served an oncogene, inducing neoplasia cultured organoids. Put together, appears promote targeting SOCS3/STAT3 signaling. Interpretation These revealed sponges inhibit development via SOCS3-mediated repression provides potential targets for diagnosis treatment CRC.

Язык: Английский

Процитировано

42
Long non-coding RNAs in cancer: multifaceted roles and potential targets for immunotherapy DOI
Lokesh Kadian, Deepika Verma, Neelam Lohani

и другие.

Molecular and Cellular Biochemistry, Год журнала: 2024, Номер 479(12), С. 3229 - 3254

Опубликована: Фев. 28, 2024

Язык: Английский

Процитировано

9
Research Progress of Long Non-Coding RNA in Tumor Drug Resistance: A New Paradigm DOI Creative Commons
Jing Zhang,

Le Wu,

Chenchen Wang

и другие.

Drug Design Development and Therapy, Год журнала: 2024, Номер Volume 18, С. 1385 - 1398

Опубликована: Апрель 1, 2024

In the past few decades, chemotherapy has been one of most effective cancer treatment options. Drug resistance is currently greatest obstacles to treatment. Even though drug mechanisms have extensively investigated, they not fully elucidated. Recent genome-wide investigations revealed existence a substantial quantity long non-coding RNAs (lncRNAs) transcribed from human genome, which actively participate in numerous biological processes, such as transcription, splicing, epigenetics, cell cycle, differentiation, development, pluripotency, immune microenvironment. The abnormal expression lncRNA considered contributing factor resistance. Furthermore, may be influenced by genetic and epigenetic variations, well individual differences patient response, attributable polymorphisms metabolic enzyme genes. This review focuses on mechanism lncRNAs target drugs study tumors with high mortality, aiming establish theoretical foundation for targeted therapy.

Язык: Английский

Процитировано

8