Targeting corticotropin-releasing hormone receptor type 1 (Crhr1) neurons: validating the specificity of a novel transgenic Crhr1-FlpO mouse DOI Creative Commons
M P Hardy, Yuncai Chen, Tallie Z. Baram

и другие.

Brain Structure and Function, Год журнала: 2024, Номер 230(1)

Опубликована: Дек. 18, 2024

Corticotropin-releasing hormone (CRH) signaling through its cognate receptors, CRHR1 and CRHR2, contributes to diverse stress-related functions in the mammalian brain. Whereas CRHR2 is predominantly expressed choroid plexus blood vessels, abundantly neurons discrete brain regions, including neocortex, hippocampus nucleus accumbens. Activation of influences motivated behaviors, emotional states, learning memory. However, it unknown whether alterations contribute aberrant behaviors observed, for example, stressful contexts. These questions require tools manipulate selectively. Here we describe validate a novel Crhr1-FlpO mouse. Using bacterial artificial chromosome (BAC) transgenesis, engineered transgenic mouse that expresses FlpO recombinase CRHR1-expressing cells. We used two independent methods assess specificity First, injected mice with Flp-dependent viruses expressing fluorescent reporter molecules. Additionally, crossed molecules were identified using immunocytochemistry visualized via confocal microscopy several regions which expression function established. Expression viral constructs was highly specific cells all examined (over 90% co-localization). In accord, robust observed mouse, recapitulating endogenous expression. The enables selective genetic access within When combined Cre-lox or site-specific recombinases, facilitates intersectional manipulations neurons.

Язык: Английский

Glutamatergic synaptic plasticity in medial vestibular nuclei during vestibular compensation DOI

Yang-Xun Zhang,

Luyao Li, Yue Xing

и другие.

Neuroscience, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Stereoelectroencephalography (SEEG)-guided insula resections: is it “Reily” worth it? DOI
Joshua Pepper, Stefano Seri, A. Richard Walsh

и другие.

Child s Nervous System, Год журнала: 2024, Номер 41(1)

Опубликована: Дек. 9, 2024

Язык: Английский

Процитировано

0
The cognitive functions of the cerebellum and its role in neurodegenerative diseases DOI Open Access

Yun-Yong Xie,

Xiaoyang Zhang, Jing‐Ning Zhu

и другие.

Ageing and Neurodegenerative Diseases, Год журнала: 2024, Номер 4(4)

Опубликована: Дек. 13, 2024

The cerebellum has long been associated with motor control. However, its role in cognitive functions attracted increasing attention recently. uniformity of cerebellar internal structure seems at odds involvement such diverse functions. Nonetheless, diseases as ataxia, there is a comorbidity and impairments, raising essential questions about how to what extent the participates This review begins by tracing historical development research, suggesting that connections between cerebral cortex, basal ganglia, other subcortical nuclei form basis for cerebellum’s regulating We then delve into language, reward-based learning, working memory, spatial cognition. Additionally, we summarize changes observed Alzheimer’s disease (AD), Parkinson’s (PD), ataxias their impact on By discussing mechanism cognition physiology pathology from aspects function, aim shed light promising new therapeutic targets related impairment.

Язык: Английский

Процитировано

0
Targeting corticotropin-releasing hormone receptor type 1 (Crhr1) neurons: validating the specificity of a novel transgenic Crhr1-FlpO mouse DOI Creative Commons
M P Hardy, Yuncai Chen, Tallie Z. Baram

и другие.

Brain Structure and Function, Год журнала: 2024, Номер 230(1)

Опубликована: Дек. 18, 2024

Corticotropin-releasing hormone (CRH) signaling through its cognate receptors, CRHR1 and CRHR2, contributes to diverse stress-related functions in the mammalian brain. Whereas CRHR2 is predominantly expressed choroid plexus blood vessels, abundantly neurons discrete brain regions, including neocortex, hippocampus nucleus accumbens. Activation of influences motivated behaviors, emotional states, learning memory. However, it unknown whether alterations contribute aberrant behaviors observed, for example, stressful contexts. These questions require tools manipulate selectively. Here we describe validate a novel Crhr1-FlpO mouse. Using bacterial artificial chromosome (BAC) transgenesis, engineered transgenic mouse that expresses FlpO recombinase CRHR1-expressing cells. We used two independent methods assess specificity First, injected mice with Flp-dependent viruses expressing fluorescent reporter molecules. Additionally, crossed molecules were identified using immunocytochemistry visualized via confocal microscopy several regions which expression function established. Expression viral constructs was highly specific cells all examined (over 90% co-localization). In accord, robust observed mouse, recapitulating endogenous expression. The enables selective genetic access within When combined Cre-lox or site-specific recombinases, facilitates intersectional manipulations neurons.

Язык: Английский

Процитировано

0