Oleanolic Acid Slows Down Aging Through IGF-1 Affecting the PI3K/AKT/mTOR Signaling Pathway

Molecules, Год журнала: 2025, Номер 30(3), С. 740 - 740

Опубликована: Фев. 6, 2025

A pentacyclic triterpene, oleanolic acid (OA), has anti-inflammatory activity. The role of in aging is poorly understood, and the regulatory mechanism IGF-1 signaling still not fully understood. Thus, we hypothesized that OA could delay by regulating PI3K/AKT/mTOR pathway via insulin-like growth factor-1 (IGF-1). This study initially established a replicative model bleomycin-induced human dermal fibroblast (HDF) mouse embryonic (MEF) cell lines. On this basis, inhibitors or recombinant proteins were then combined with (at concentration 20 μM) treated for 72 h. project plans to detect expression aging-related such as CDKN2A (p16) using Western blot technology, factors Interleukin-1 beta (IL-1β), Interleukin-6 (IL-6), Interleukin-8 (IL-8) Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR), Enzyme-Linked Immunosorbent Assay (ELISA), other technologies, combine Senescence-Associated β-Galactosidase (SA-β-gal) staining changes aging. IGF-1, PI3K/AKT/mTOR, P16, secretory (SASP) IL-1β, IL-6, IL-8 was increased senescent cells. After treatment jujuboside, protein decreased. findings suggested slowed down inhibiting through IGF-1. These suggest potential new drug its mechanisms anti-aging.

Язык: Английский

Advances in Insulin Resistance—Molecular Mechanisms, Therapeutic Targets, and Future Directions

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2574 - 2574

Опубликована: Март 13, 2025

The development of insulin resistance (IR) is characterized by a series metabolic disturbances, including, but not limited to, impaired glucose uptake, increased blood sugar levels, and disrupted lipid metabolism [...]

Язык: Английский