The SLOMO F-Box Protein is Required for ABA-Induced Degradation of VAMP721/722 in Arabidopsis

Journal of Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 7, 2025

Язык: Английский

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HOS15 impacts DIL9 protein stability during drought stress in Arabidopsis

New Phytologist, Год журнала: 2025, Номер unknown

Опубликована: Янв. 31, 2025

Summary HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 15 (HOS15) acts as a substrate receptor of E3 ligase complex, which plays negative role in drought stress tolerance. However, whether and how HOS15 participates controlling important transcriptional regulators remains largely unknown. Here, we report that physically interacts with tightly regulates DROUGHT‐INDUCED LIKE 19 (DIL9) protein stability. Moreover, application exogenous abscisic acid (ABA) stabilizes the interaction between DIL9 HOS15, leading to ABA‐induced proteasomal degradation by HOS15. Genetic analysis revealed functions downstream tolerance hos15‐2 plants was impaired dil9/hos15 double mutants. Notably, is directly associated promoter regions ABF transcription factors facilitates their expression, pivotal enhancing ABA‐dependent Collectively, these findings demonstrate consistently degrades under normal condition, while (drought/ABA) promotes activity for binding ABFs positively expression response dehydration.

Язык: Английский

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Transcriptomic Analysis Reveals Adaptive Evolution and Conservation Implications for the Endangered Magnolia lotungensis

Genes, Год журнала: 2024, Номер 15(6), С. 787 - 787

Опубликована: Июнь 14, 2024

Magnolia lotungensis is an extremely endangered endemic tree in China. To elucidate the genetic basis of M. lotungensis, we performed a comprehensive transcriptome analysis using sample integrating plant’s bark, leaves, and flowers. De novo assembly yielded 177,046 transcripts 42,518 coding sequences. Notably, identified 796 species-specific genes enriched organelle gene regulation defense responses. A codon usage bias revealed that mutation appears to be primary driver selection shaping species’ architecture. An evolutionary based on dN/dS values paralogous orthologous pairs indicated predominance purifying selection, suggesting strong constraints most genes. comparative transcriptomic with sinica approximately 1000 ultra-conserved genes, essential cellular processes such as transcriptional regulation, protein synthesis, genome stability. Interestingly, only limited number 511 rapidly evolving under positive were detected compared kuangsiensis. These metabolic associated adaptation specific environments, potentially limiting ability expand its range. Our findings contribute understanding architecture suggest insufficient adaptive status.

Язык: Английский

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Genome-wide identification and expression profiling of the NCED gene family in salt and drought stress response of honeysuckle (Lonicera japonica thunb.)

Scientia Horticulturae, Год журнала: 2025, Номер 341, С. 113980 - 113980

Опубликована: Янв. 18, 2025

Язык: Английский

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Editorial: Genetic regulatory mechanisms of osmotic stress response in plants

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Янв. 28, 2025

Li et al. (2023) examined the role of NAC transcription factor MdNAC29 in apples, revealing its negative regulation drought tolerance. Overexpression resulted increased oxidative damage, reduced chlorophyll content, and downregulation droughtresponsive genes like MdDREB2A. The interaction between F-box protein MdPP2-B10 further highlighted regulatory transcriptional repression under conditions. This study underscores complexity factor-mediated gene expression provides a basis for improving tolerance through genetic engineering (Li al., 2023).Xu ( 2023) identified novel catalase gene, PtCAT2, from Pinellia ternata, which enhances Arabidopsis thaliana. PtCAT2 activity by five-fold, leading to improveed reactive oxygen species (ROS) scavenging reduction damage. highlights pivotal ROS balance osmotic stress responses positions as candidate interventions drought-sensitive crops (Xu 2023).Gao employed proteomic analysis elucidate leaves roots foxtail millet (Setaria italica). significant differences, with primarily altering photosynthesis-related proteins modifying greater number involved metabolites metabolism stress-defense during both recovery phases. These findings underscore importance tissue-specific adaptations development drought-stress tolerant (Gao 2023). Piríz-Pezzutto 2024) developed an innovative vitro gradient system root adaptation natural field conditions, where encounter increasing potential while exploring soil. revealed that grown gradients sustained higher growth rates exhibited distinct changes certain compared those subjected uniform shock. Findings this emphasize mimicking conditions uncover mechanisms adaptive (Piríz-Pezzutto 2024).Guo explored function MdKAI2, receptor karrikins (KARs), regulating resistance apples. demonstrated MdKAI2 positively regulates enhancing scavenging, promoting flavonoid biosynthesis, osmoregulatory substances. RNA-sequencing MdKAI2-overexpressing apple calli modulates various factors MAPK signaling pathway. open avenues leveraging KAR-related pathways improve tree crop resilience (Guo 2024).To investigate influence ploidy levels on salt citrus, Bonnin conducted comparative transcriptomic diploid tetraploid citrus genotypes stress. Tetraploid enhanced differential cell wall remodeling, sugar metabolism, antioxidant responses. research polyploidy abiotic perennial fruit (Bonnin 2024).These studies collectively advance our understanding response across diverse plant systems. They highlight factors, enzymatic regulators, changes, genomic mitigating Future should prioritize integrative multi-omics approaches, field-like experimental designs, translation into improvement programs (Seok

Язык: Английский

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The Mulberry WRKY Transcription Factor MaWRKYIIc7 Participates in Regulating Plant Drought Stress Tolerance

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1714 - 1714

Опубликована: Фев. 17, 2025

The sericulture industry is an important component of the agricultural industry. Drought stress can cause yellowing, premature ageing, and shrinkage mulberry (Morus alba L.) leaves, greatly damaging their quality restricting high-quality development WRKY transcription factors play roles in plant drought response. In this study, we found that MaWRKYIIc7 TFs, had significantly higher expression levels leaves than other tissues was induced to be expressed under stress. protein located nucleus plasma membrane, its transcriptional activity depends mainly on N-terminal sequence. overexpression Arabidopsis resulted better tolerance. An analysis transient seedlings revealed transgenic presented decreased stomatal opening, MDA content, increased ROS clearance ability, ABA biosynthesis-related genes. Y1H Dual-luc results indicate bind W-boxes positively regulate MaNCED1 MaRD29A, synergistically regulating tolerance mulberry. Overall, our research suggests increase by promoting clearance, adjusting activating signalling pathway.

Язык: Английский

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Genome-wide identification and expression analysis of TaFDL gene family responded to vernalization in wheat (Triticum aestivum L.)

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Март 16, 2025

FLOWERING LOCUS D (FD) is a basic leucine zipper (bZIP) transcription factor known to be crucial in vernalization, flowering, and stress response across variety of plants, including biennial winter annual species. The TaFD-like (TaFDL) gene wheat the functional homologue Arabidopsis FD, yet research on TaFDL family still lacking. In this study, total 62 members were identified classified into 4 main subfamilies, these genes located 21 chromosomes. A comprehensive analysis physicochemical properties, structure, conservation motif, conserved domain, advanced protein structure revealed among its individual subfamily. underwent purifying selection. segmental duplication events driving force behind expansion family. differentiation evolution FD genes. Additionally, subcellular localization transcriptional activation activities five key demonstrated. Gene Ontology (GO) annotations promoter cis-regulatory element indicated that may play potential roles regulating hormone response, low-temperature light which verified by transcriptome data analysis. Specifically, quantitative real-time PCR (qRT-PCR) exhibited differential responses different vernalization conditions seeding. Finally, homologous nine cultivars highlight significant genetic diversity. These findings enrich genes, providing valuable insights family's vernalization.

Язык: Английский

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Synergistic Effect of Serratia fonticola and Pseudomonas koreensis on Mitigating Salt Stress in Cucumis sativus L.

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(3), С. 194 - 194

Опубликована: Март 15, 2025

Beneficial microbes enhance plant growth and development, even under stressful conditions. Serratia fonticola (S1T1) Pseudomonas koreensis (S4T10) are two multi-trait growth-promoting rhizobacteria (PGPRs) that resistant to saline This study evaluated the synergistic effect of these PGPRs on mitigating salinity stress (200 mM) in Cucumis sativus. Presently, both strains enhances attributes cucumber, parameters were significantly higher than those uninoculated plants. The PGPR-treated plants revealed a biomass improved chlorophyll content. inoculation S1T1 S4T10 promoted 23, 24, 28% increases, respectively, fresh 16, 19.8, 24% dry biomass. Similarly, their effects led 16.5, 28.4, 38% water potential 18, 22, decreases, abscisic acid (ABA). A reduction electrolytic leakage (EL) was additional proof successful PGPR activities. decrease antioxidant levels, including malondialdehyde (21–30%), hydrogen peroxide (19–38%), superoxide anions (24–34%), observed, alongside an increase enzymes such as catalase (22–29%) dismutase (17–27%). Additionally, enhanced NaCl tolerance by upregulating expression ion transporter genes HKT1 (1–2-fold), NHX (1–3-fold), SOS1 (2–4-fold). Conclusively, C. sativus L. salt stress.

Язык: Английский

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Identification and expression profiles of tubby‑like proteins coding genes in Brassica rapa (B. rapa) in response to hormone and drought stress

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Май 5, 2025

Tubby-like proteins (TLPs) are a widespread multigene family found in single-celled to multicellular eukaryotes. Despite their significance, no reports of TLPs B. rapa have been made up this point. Herein, we identified 14 the genome and renamed them BrTUB1-BrTUB14 based on chromosomal location. The bulk BrTUB contain two characteristic domains: F-box Tubby domains. Subcellular localization prediction confirmed that BrTUBs localized nucleus. Expression profiling showed many reacts variety stressors, including drought stress hormonal treatments (ABA ethylene). In particular, BrTUB1 displayed elevated expression ABA treatment. This study is first thorough identification family, providing critical insights into its function regulation, laying groundwork for future functional analyses, particularly concerning tolerance rapa.

Язык: Английский

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Past trauma, better future: how stress memory shapes plant adaptation to drought

Functional Plant Biology, Год журнала: 2025, Номер 52(6)

Опубликована: Май 15, 2025

Can plants remember drought? Emerging evidence suggests that prior stress exposure leaves an epigenetic imprint, reprogramming for enhanced resilience. However, the stability and functional relevance of drought memory remain unresolved. This review synthesizes recent advances in modifications, transcriptional reprogramming, metabolic priming, critically assessing their roles plant adaptation. DNA methylation dynamically reshapes chromatin landscapes, yet its transient nature questions long-term inheritance. Histone particularly H3K9ac H2Bub1, may encode signatures, enabling rapid responses, whereas small RNAs fine-tune states to reinforce memory. Beyond epigenetics, physiological including osmotic adjustments, antioxidant defenses, hormonal crosstalk, introduces further complexity, evolutionary advantage remains unclear. Root system plasticity enhance resilience, but trade-offs underpinnings are largely unexplored. A critical challenge is disentangling stable adaptive mechanisms from acclimatory shifts. We propose a framework evaluating across temporal generational scales highlight potential precision genome editing establish causality. By integrating multi-omics, gene editing, field-based validation, this aims unlock molecular blueprint Understanding these key engineering climate-resilient crops, ensuring global food security era increasing environmental uncertainty.

Язык: Английский

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