The Safe Return of Face-to-Face Teaching in the Post-COVID-19 Era at a University in Southern Italy: Surface Monitoring as an Early Warning System DOI Creative Commons
Osvalda De Giglio, Francesco Triggiano, Francesca Apollonio

и другие.

Applied Sciences, Год журнала: 2023, Номер 13(24), С. 13214 - 13214

Опубликована: Дек. 13, 2023

Environmental monitoring for SARS-CoV-2 has become a useful adjunct to clinical testing because it is widely available and relatively inexpensive. During the period May–December 2022 (spring–summer: May–September–autumn: October–December), we assessed presence viability of virus on surfaces in university settings Apulia region (Southern Italy) after resumption face-to-face teaching activities evaluated surface as an early warning system. The sampling plan provided selection 75% types (e.g., student teacher desks, computer, handrail) different materials (plasticized wood, metal, plastic) present environments. Overall, 5.4% (all students’ desks) resulted positive with RT-PCR negative viral culture. Greater contamination was found spring–summer than autumn (χ2 test Yates correction = 7.6003; p-value 0.006). Poisson regression model showed direct association between average number COVID-19 cases among students seven days following percentage swabs day (Intercept 5.32498; β 0.01847; p < 0.001). Our results show that environmental SARS-CoV-2, especially crowded such universities, could be tool warning, even end emergency.

Язык: Английский

Assessment of environmental factors influencing SARS-CoV-2 in Vietnam's surface water across two years of clinical data DOI Creative Commons

Yadpiroon Siri,

Bikash Malla, Le Thanh Thao

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 957, С. 177449 - 177449

Опубликована: Ноя. 19, 2024

Wastewater-based epidemiology (WBE) is an effective, non-invasive method for monitoring the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by tracking viral prevalence in water. This study aimed to investigate presence SARS-CoV-2 surface water Vietnam over two years. One-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays were employed quantify and its variant-specific mutation sites (G339D/E484A) pepper mild mottle virus (PMMoV) from a total 315 samples (105 per type) compare with reported Coronavirus disease 2019 (COVID-19) cases environmental factors. was detected 38 % (40/105), 43 (45/105), 39 (41/105) Sites A, B, C, respectively, concentrations 3.0-5.6 log

Язык: Английский

Процитировано

2
Wastewater Surveillance of SARS-CoV-2 in Zambia: An Early Warning Tool DOI Open Access
Doreen Mainza Shempela, Walter Muleya, Steward Mudenda

и другие.

Опубликована: Июнь 26, 2024

Wastewater-based surveillance has emerged as an important method for monitoring SARS-CoV-2. This study investigated the presence of SARS-CoV-2 in wastewater Zambia. We conducted a longitudinal Copperbelt and Eastern provinces Zambia from October 2023 to December during which 155 samples were collected. The subjected three different concentration methods, namely bag-mediated filtration, skimmed milk flocculation, polythene glycol-based assays. Molecular detection nucleic acid was using real-time PCR. Whole genome sequencing Illumina COVIDSEQ assay. Of samples, 62 (40%) tested positive these, 13 sequences sufficient length determine lineages obtained two phylogenetically analyzed. Various Omicron subvariants detected including BA.5, XBB.1.45, BA.2.86, JN.1. Some these have been clinical cases Interestingly, phylogenetic analysis positioned sequence Province B.1.1.529 clade, suggesting that earlier variants late 2021 could still be circulating may not wholly replaced by newer subvariants. stresses need integrating into mainstream strategies circulation

Язык: Английский

Процитировано

1
The reemergence of measles and the urgent need for uninterrupted genetic surveillance and vaccination DOI
Francesco Branda, Marta Giovanetti, Chiara Romano

и другие.

Clinical Microbiology and Infection, Год журнала: 2024, Номер 30(10), С. 1215 - 1218

Опубликована: Июль 22, 2024

Язык: Английский

Процитировано

0
Wastewater Surveillance of SARS-CoV-2 in Zambia: An Early Warning Tool DOI Open Access
Doreen Mainza Shempela, Walter Muleya, Steward Mudenda

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(16), С. 8839 - 8839

Опубликована: Авг. 14, 2024

Wastewater-based surveillance has emerged as an important method for monitoring the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). This study investigated presence of SARS-CoV-2 in wastewater Zambia. We conducted a longitudinal Copperbelt and Eastern provinces Zambia from October 2023 to December during which 155 samples were collected. The subjected three different concentration methods, namely bag-mediated filtration, skimmed milk flocculation, polythene glycol-based assays. Molecular detection nucleic acid was using real-time Polymerase Chain Reaction (PCR). Whole genome sequencing Illumina COVIDSEQ assay. Of samples, 62 (40%) tested positive SARS-CoV-2. these, 13 sequences sufficient length determine lineages obtained phylogenetically analyzed. Various Omicron subvariants detected including BA.5, XBB.1.45, BA.2.86, JN.1. Some these have been clinical cases Interestingly, phylogenetic analysis positioned sequence Province B.1.1.529 clade, suggesting that earlier variants late 2021 could still be circulating may not wholly replaced by newer subvariants. stresses need integrating into mainstream strategies circulation

Язык: Английский

Процитировано

0
The Safe Return of Face-to-Face Teaching in the Post-COVID-19 Era at a University in Southern Italy: Surface Monitoring as an Early Warning System DOI Creative Commons
Osvalda De Giglio, Francesco Triggiano, Francesca Apollonio

и другие.

Applied Sciences, Год журнала: 2023, Номер 13(24), С. 13214 - 13214

Опубликована: Дек. 13, 2023

Environmental monitoring for SARS-CoV-2 has become a useful adjunct to clinical testing because it is widely available and relatively inexpensive. During the period May–December 2022 (spring–summer: May–September–autumn: October–December), we assessed presence viability of virus on surfaces in university settings Apulia region (Southern Italy) after resumption face-to-face teaching activities evaluated surface as an early warning system. The sampling plan provided selection 75% types (e.g., student teacher desks, computer, handrail) different materials (plasticized wood, metal, plastic) present environments. Overall, 5.4% (all students’ desks) resulted positive with RT-PCR negative viral culture. Greater contamination was found spring–summer than autumn (χ2 test Yates correction = 7.6003; p-value 0.006). Poisson regression model showed direct association between average number COVID-19 cases among students seven days following percentage swabs day (Intercept 5.32498; β 0.01847; p < 0.001). Our results show that environmental SARS-CoV-2, especially crowded such universities, could be tool warning, even end emergency.

Язык: Английский

Процитировано

0