Akkermansia muciniphila- and Pathogenic Bacteria-Derived Endotoxins Differently Regulate Human Dendritic Cell Generation and γδ T Lymphocyte Activation DOI Creative Commons

Veronica Fertitta,

Barbara Varano, Manuela Del Cornò

и другие.

Biomolecules, Год журнала: 2024, Номер 14(12), С. 1571 - 1571

Опубликована: Дек. 9, 2024

Lipopolysaccharide (LPS) is a potent endotoxin released at high concentrations in acute infections, causing massive host inflammatory response. Accumulating evidence indicates that dysbiosis-associated chronic low levels of circulating LPS can sustain prolonged sterile low-grade inflammation increases the risk several non-communicable diseases. Interventions aimed increasing abundance beneficial/probiotic bacteria, including Akkermansia muciniphila, result reduced inflammation, favoring metabolic and immune health. Immunosuppression common feature conditions dendritic cells (DCs) represent key targets given their ability to shift balance toward immunity or tolerance. In this study, effects from pathogenic (Escherichia coli Salmonella enterica) probiotic (Akkermansia muciniphila) bacterial species on human DC generation functions were compared. We report monocyte precursor priming with Escherichia enterica forces differentiation PD-L1-expressing DCs, releasing IL-6 IL-10, impairs capacity drive full TCR-Vδ2 T cell activation. Conversely, comparable muciniphila promoted DCs preserved activating potential immunostimulatory properties. These results shed light mechanisms underlying impact endotoxemia disease pathogenesis, increase our understanding immunomodulatory muciniphila.

Язык: Английский

Bacterial extracellular vesicles in the initiation, progression and treatment of atherosclerosis DOI Creative Commons
Yuling Lin, Jingyu Wang, Fan Bu

и другие.

Gut Microbes, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 22, 2025

Atherosclerosis is the primary cause of cardiovascular and cerebrovascular diseases. However, current anti-atherosclerosis drugs have shown conflicting therapeutic outcomes, thereby spurring search for novel effective treatments. Recent research indicates crucial involvement oral gastrointestinal microbiota in atherosclerosis. While gut metabolites, such as choline derivatives, been extensively studied reviewed, emerging evidence suggests that bacterial extracellular vesicles (BEVs), which are membrane-derived lipid bilayers secreted by bacteria, also play a significant role this process. BEVs host-microbiota interactions remains insufficiently explored. This review aims to elucidate complex communication mediated along gut-heart axis. In review, we summarize knowledge on BEVs, with specific focus how pathogen-derived contribute promotion atherosclerosis, well from symbionts probiotics may mitigate its progression. We explore potential challenges associated engineered prevention treatment Finally, discuss benefits using atherosclerosis diagnosis treatment, propose future directions address these issues.

Язык: Английский

Процитировано

1
Segatella copri Outer-Membrane Vesicles Are Internalized by Human Macrophages and Promote a Pro-Inflammatory Profile DOI Open Access

Alison Sepúlveda-Pontigo,

Karissa Chávez-Villacreses,

Cristóbal Madrid-Muñoz

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3630 - 3630

Опубликована: Апрель 11, 2025

Increased abundance of Segatella copri (S. copri) within the gut microbiota is associated with systemic inflammatory diseases, including rheumatoid arthritis. Although outer-membrane vesicles (OMVs) Gram-negative bacteria are important players in microbiota–host communication, effect S. copri-derived OMVs on immune cells unknown. Macrophages engulf and eliminate foreign material conditioned by environmental signals to promote either homeostasis or inflammation. Thus, we aimed explore impact copri-OMVs human macrophages vitro, employing THP-1 monocyte-derived macrophage models. The uptake DiO-labeled into was monitored confocal microscopy flow cytometry. Furthermore, phenotype cytokine secretion naïve (M0), pro-inflammatory (M1), anti-inflammatory (M2) analyzed cytometry ELISA, respectively. We show that enter through macropinocytosis clathrin-dependent mechanisms. copri-OMVs, but not parental bacterium, induced a dose-dependent increase expression M1-related surface markers M0 M2 activated large amounts cytokines M1 macrophages. These results highlight an role promoting responses, which might contribute diseases.

Язык: Английский

Процитировано

0
Akkermansia muciniphila extracellular vesicles: function and theranostic potential in disease DOI Creative Commons
Yudi Wang, Wenlong Lin, Shaohuan Wu

и другие.

LabMed discovery., Год журнала: 2025, Номер unknown, С. 100060 - 100060

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
A comprehensive update on the immunoregulatory mechanisms of Akkermansia muciniphila : insights into active ingredients, metabolites, and nutrient-driven modulation DOI

Lihua Mei,

Jiaxin Wang,

Yanling Hao

и другие.

Critical Reviews in Food Science and Nutrition, Год журнала: 2024, Номер unknown, С. 1 - 18

Опубликована: Окт. 16, 2024

Akkermansia muciniphila (A. muciniphila) has gained recognition as a pioneering probiotic, exhibiting considerable potential to enhance immune conditions across both humans and animals. The health benefits of A. are attributed its various components, including outer membrane proteins (PilQ Amuc_1100), secreted (P9 AmTARS), extracellular vesicles, metabolites such SCFAs, ornithine lipids, γ-aminobutyric acid, cobalamin, inosine. dynamic control the mucus layer by plays crucial role in regulating intestinal mucosal immunity. Furthermore, modulates function interacting with macrophages, dendritic cells, T lymphocytes, Paneth cells. Increasing abundance gut through nutritional strategies represents safe effective means augment function. Various polyphenols, oligosaccharides, polysaccharides have been shown elevate levels this bacterium, thereby contributing favorable immunoregulatory outcomes. This paper delves into latest research advancements related probiotic mechanisms provides an overview current understanding how responds nutrients. These insights offer theoretical foundation for utilization immunoregulation.

Язык: Английский

Процитировано

3
A protocol to isolate and characterize pure monocytes and generate monocyte-derived dendritic cells through FBS-Coated flasks DOI Creative Commons
Maryam Meskini, Amir Amanzadeh, Fahimeh Salehi

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Окт. 14, 2024

This study explores methods to isolate high-pure monocytes and optimize the best growth factor concentration generate monocytes-derived dendritic cells (mo-DCs), subset DC1, which is crucial in immune responses. Three protocols for monocyte isolation from peripheral blood mononuclear (PBMCs) were evaluated: three-hour incubation on FBS-coated flasks; an overnight Magnetic Activated Cell Sorting (MACS). Additionally, five different concentrations of human recombinant granulocyte-macrophage colony-stimulating (hrGM-CSF) interleukin-4 (hrIL-4) compared. We used Flow cytometry assess isolation, purification, generation pure characterized as CD14+, expression mo-DC classical markers (HLA-DR, CD80, CD83, CD86). The obtained results show that isolated with second method (overnight incubation) had highest purity (P < 0.0001) but lowest yield > 0.05), balancing cost-effectiveness. A combination hrGM-CSF hrIL-4 at 400 U/mL produced most favorable outcomes, leading rate 0.05). Notably, this resulted increasing HLA-DR, CD86 surface generated DCs 0.0001), no changes CD83 levels. In conclusion, offers valuable insights into selecting optimal approach various research contexts, providing a foundation more effective immunological studies.

Язык: Английский

Процитировано

1
Oral Pathobiont-Derived Outer Membrane Vesicles in the Oral–Gut Axis DOI Open Access

Eduardo A. Catalán,

Emilio Seguel-Fuentes,

Brandon Fuentes

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(20), С. 11141 - 11141

Опубликована: Окт. 17, 2024

Oral pathobionts are essential in instigating local inflammation within the oral cavity and contribute to pathogenesis of diseases gastrointestinal tract other distant organs. Among Gram-negative pathobionts,

Язык: Английский

Процитировано

0
Akkermansia muciniphila- and Pathogenic Bacteria-Derived Endotoxins Differently Regulate Human Dendritic Cell Generation and γδ T Lymphocyte Activation DOI Creative Commons

Veronica Fertitta,

Barbara Varano, Manuela Del Cornò

и другие.

Biomolecules, Год журнала: 2024, Номер 14(12), С. 1571 - 1571

Опубликована: Дек. 9, 2024

Lipopolysaccharide (LPS) is a potent endotoxin released at high concentrations in acute infections, causing massive host inflammatory response. Accumulating evidence indicates that dysbiosis-associated chronic low levels of circulating LPS can sustain prolonged sterile low-grade inflammation increases the risk several non-communicable diseases. Interventions aimed increasing abundance beneficial/probiotic bacteria, including Akkermansia muciniphila, result reduced inflammation, favoring metabolic and immune health. Immunosuppression common feature conditions dendritic cells (DCs) represent key targets given their ability to shift balance toward immunity or tolerance. In this study, effects from pathogenic (Escherichia coli Salmonella enterica) probiotic (Akkermansia muciniphila) bacterial species on human DC generation functions were compared. We report monocyte precursor priming with Escherichia enterica forces differentiation PD-L1-expressing DCs, releasing IL-6 IL-10, impairs capacity drive full TCR-Vδ2 T cell activation. Conversely, comparable muciniphila promoted DCs preserved activating potential immunostimulatory properties. These results shed light mechanisms underlying impact endotoxemia disease pathogenesis, increase our understanding immunomodulatory muciniphila.

Язык: Английский

Процитировано

0