Impact of Probiotics on Enzyme Activities, Intestinal Microbial Remodeling, and Metabolic Pathways in American Shad (Alosa sapidissima) at High Temperatures
Marine Biotechnology,
Год журнала:
2025,
Номер
27(2)
Опубликована: Март 3, 2025
Язык: Английский
Influence of dietary inulin on growth and intestinal ecology in Pacific white shrimp (Litopenaeus vannamei)
Jungyeol Park,
Huirong Lv,
C. Young Cho
и другие.
Journal of the World Aquaculture Society,
Год журнала:
2025,
Номер
56(2)
Опубликована: Апрель 1, 2025
Abstract
This
study
investigates
the
influence
of
dietary
inulin
on
growth
and
health
juvenile
shrimp
Litopenaeus
vannamei
over
an
8‐week
trial,
focusing
growth,
health,
metabolic
impacts
because
diet
variation.
A
total
240
with
initial
mean
weight
0.26
±
0.01
g
were
distributed
across
12
recirculating
tanks
assigned
diets
varying
concentrations:
0%
(Control),
0.5%
(T1),
1.0%
(T2)
1.5%
(T3).
Results
indicated
that
resulted
in
highest
specific
rate,
gain,
survival
rate.
An
increase
antioxidative
immune
enzyme
activities
suggested
improved
conditions
at
optimal
levels,
evidenced
by
reduction
malondialdehyde
content
potentiation
enzymes
like
superoxide
dismutase
lysozyme.
Microbiome
analysis
via
16S
rRNA
sequencing
showed
a
significant
shift
gut
bacterial
populations,
beneficial
bacteria
such
as
Xanthomarina
pathogenic
Vibrio
Flavobacteria
inulin‐supplemented
groups.
Higher
increased
microflora
diversity
significantly
T2
group.
Metabolomic
distinct
pathway
enrichment
differential
metabolite
regulation,
linking
intake
to
alterations
lipid
metabolism,
including
pathways
bile
secretion,
arachidonic
acid
steroid
hormone
biosynthesis,
ABC
transporters.
Furthermore,
correlation
KEGG
analyses
revealed
intricate
interactions
within
network,
pointing
compensatory
regulatory
mechanisms
response
supplementation.
In
conclusion,
substantially
improves
L.
,
likely
modulating
microbiota
pathways.
highlights
potential
supplement
for
enhancing
aquaculture
efficacy.
Язык: Английский
Antimicrobial Activity, Genetic Diversity and Safety Assessment of Lactic Acid Bacteria Isolated from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean
Antibiotics,
Год журнала:
2025,
Номер
14(5), С. 469 - 469
Опубликована: Май 6, 2025
Background/Objectives:
The
overuse
and
misuse
of
antibiotics
has
contributed
significatively
to
the
growing
problem
emergence
spread
antibiotic
resistance
genes
among
bacteria,
posing
a
serious
global
challenge
treatment
bacterial
infectious
diseases.
For
these
reasons,
there
is
current
interest
in
development
effective
alternative
or
complementary
strategies
therapy
for
prevention
fish
diseases,
which
are
mainly
based
on
use
probiotics—in
particular,
those
belonging
Lactic
Acid
Bacteria
(LAB)
group.
In
this
context,
aim
present
study
was
characterise,
evaluate
genetic
diversity
assess
safety
candidate
probiotic
LAB
strains
aquaculture
isolated
from
faeces
intestines
European
hakes
(Merluccius
merluccius,
L.)
caught
Northeast
Atlantic
Ocean
(Ireland).
Methods:
direct
antimicrobial
activity
isolates
tested
by
Stab-On-Agar
method
against
key
ichthyopathogens.
Subsequently,
their
taxonomic
classification
were
determined
16SrDNA
sequencing
Enterobacterial
Repetitive
Intergenic
Consensus-PCR
(ERIC-PCR),
respectively.
To
ensure
vitro
isolates,
biofilm-forming
ability
assessed
microtiter
plate
assay;
sensitivity
major
used
aquaculture,
human
veterinary
medicine
broth
microdilution
haemolytic
gelatinase
microbiological
assays.
Results:
All
biofilm
producers
susceptible
chloramphenicol,
oxytetracycline,
flumequine
amoxicillin.
A
total
30
(85.7%)
resistant
at
least
one
antibiotics.
None
35
showed
proteolytic
activity.
Conclusions:
Among
strains,
five
exhibiting
highest
aquaculture-relevant
ichthyopathogens,
taxonomically
identified
as
Streptococcus
salivarius,
Enterococcus
avium
Latilactobacillus
sakei,
selected
further
characterisation
potential
candidates
promote
sustainable
aquaculture.
our
knowledge,
first
report
that
hake
represent
viable
ecological
niches
isolation
with
Язык: Английский
Effects of Dietary Supplementation with Bacillus subtilis natto on Growth, Digestive Enzyme Activity, Immune Response, and Intestinal Microorganisms of Red Sea Bream, Pagrus major
Fishes,
Год журнала:
2024,
Номер
9(11), С. 446 - 446
Опубликована: Окт. 31, 2024
This
study
investigated
the
influence
of
dietary
supplementation
with
Bacillus
subtilis
natto
(BSN)
on
various
physiological
parameters
in
red
sea
bream
(Pagrus
major).
Key
areas
focus
included
growth
performance,
enzymatic
activity
related
to
digestion,
blood
biochemical
markers,
immune
function,
and
intestinal
microorganisms.
Fish
were
fed
diets
containing
following
five
levels
BSN:
0
(BN0),
1
×
107
(BN1),
108
(BN2),
109
(BN3),
1010
(BN4)
CFU
kg−1
for
56
days.
BN3
BN4
groups
exhibited
significantly
enhanced
performance
compared
BN0
group
(p
<
0.05).
Additionally,
activities
amylase
protease
markedly
higher
0.05),
while
lipase
was
elevated
fish
diet
Plasma
total
protein
also
showed
a
significant
increase
Hematocrit
values
improved
BN2,
BN3,
The
microbiota
revealed
populations
B.
Lactobacillus,
along
notable
decrease
Escherichia
coli,
all
other
observed
improvements
specific
rate
upregulation
growth-related
gene
expression
further
highlight
potential
BSN
enhance
bream.
In
conclusion,
at
shows
promise
improving
growth,
health,
response
Язык: Английский
Biotechnological Tools – Advances and Diagnostics to Control Parasitic Diseases in Aquaculture
Shukla Devnath,
Souvik Dhar,
Anjana Verma
и другие.
Опубликована: Янв. 1, 2024
Язык: Английский
Potential Probiotic Bacillus Strains with Antioxidant and Antimutagenic Activity Increased Weight Gain and Altered hsp70, cxc, tnfα, il1β, and lysC Gene Expression in Clarias gariepinus
Fishes,
Год журнала:
2024,
Номер
9(12), С. 476 - 476
Опубликована: Ноя. 25, 2024
The
potential
probiotic
properties
of
three
Bacillus
strains
were
studied.
A
supplement
for
the
African
catfish
Clarias
gariepinus
was
produced
via
solid-state
fermentation
protocol
and
incorporated
into
fish
feed
a
period
seven
weeks.
Since
36th
day
experiment,
all
experimental
groups
had
statistically
significant
increase
in
their
weight
gain
than
control
group.
maximum
observed
fed
probiotic-supplemented
29.16%
higher
that
group,
conversion
rate
improvement
24%.
Cell-free
extracts
from
these
showed
antioxidant
(11.55–27.40%)
DNA-protective
(45.33–61.83%)
activity
series
vitro
biosensor
tests.
Further
investigation
antimutagenic
revealed
two
them
reduced
level
induced
mutagenesis
an
Escherichia
coli
model
(by
33.58%
54.35%,
respectively).
We
also
assessed
impact
on
expression
several
key
genes
host
(C.
gariepinus),
including
hsp70,
cxc,
tnfα,
il1β,
lysC.
More
10-fold
rates
hsp70
gonads
liver;
cxc
muscles
gonads;
tnfα
brain,
gills,
il1β
gonads,
lysC
liver,
muscles.
This
study
provides
evidence
probiotics
exhibiting
can
provide
benefits
vivo
within
aquaculture
systems.
molecular
effects
appear
to
be
complex
tissue-specific,
with
both
upregulation
downregulation
immune
system
observed.
Nevertheless,
at
organismal
level,
unequivocally
positive
terms
objectives,
manifested
as
enhanced
body
fish.
Consequently,
warrant
serious
consideration
this
species.
Язык: Английский
Beneficial and Safety Properties of a Bacteriocinogenic and Putative Probiotic Latilactobacillus sakei subsp. sakei 2a Strain
Foods,
Год журнала:
2024,
Номер
13(23), С. 3770 - 3770
Опубликована: Ноя. 25, 2024
This
work
aimed
to
evaluate
some
of
the
probiotic
features
and
safety
bacteriocin-producing
Язык: Английский