Proteomic Analysis of Retinas in a Rat Model of High-Fat Diet-Induced Type 2 Diabetes: Implications of Interventional Targets for Nonproliferative Diabetic Retinopathy DOI Creative Commons
Xinxin Pang,

Siqiong Bai,

Zhinan Feng

и другие.

Drug Design Development and Therapy, Год журнала: 2025, Номер Volume 19, С. 2979 - 2999

Опубликована: Апрель 1, 2025

This study aimed to establish a high-fat diet (HFD)-induced rat model of type 2 diabetes mellitus (T2DM) and employed tandem mass tag (TMT) proteomics search for novel interventional targets nonproliferative diabetic retinopathy (NPDR). Six-week-old male Sprague-Dawley rats were randomly divided into T2DM group fed HFD normal (NOR group) chow. After 6 w, the was confirmed have impaired glucose tolerance intraperitoneally injected with single small dose streptozotocin (STZ, 30 mg/kg), blood levels monitored. The maintained another an Evans blue assay dark-adapted electroretinogram (ERG) conducted. Rat retinas collected morphology analysis, TMT Western blotting. expression patterns selected differentially expressed proteins (DEPs) validated in via blotting aqueous humor from NPDR patients slot 12-w STZ injection, presented typical symptoms T2DM. pathological features NPDR, including compromised scotopic ERGs, thinning retinal layers, increased apoptosis vascular leakage retina. Proteomic analysis identified DEPs revealed profound dyslipidemia retinas. significant upregulation FABP3, TINAGL1, COL4A3 by In HFD-induced that is consistent natural history bioinformatics analyses as 3 key upregulated first time. These findings are supported technical clinical validations provide intervention.

Язык: Английский

FABP3 promotes cell apoptosis and oxidative stress by regulating ferroptosis in lens epithelial cells DOI
Qi Wang, Chunxiao Zhang, Bin Yu

и другие.

Free Radical Research, Год журнала: 2025, Номер unknown, С. 1 - 14

Опубликована: Март 5, 2025

The purpose of this study is to investigate FABP3's biological function and potential mechanism in cataract. Treatment H2O2 raised FABP3 expression. decreased cell viability, enhanced apoptosis, promoted Bax cleaved caspase-3 expression, inhibited Bcl-2 the levels IL-6, IL-1β, TNF-α, MDA level, SOD GSH HLE-B3 cells. However, effects on inflammatory cytokines, oxidative stress were reversed by knockdown aggravated overexpression. increased lipid hydroperoxides Fe2+, but reduced expression GPX4, SLC7A11, Ferritin protein. Nevertheless, changes hydroperoxides, protein, overexpression caused opposite results. In addition, inhibition inflammation, was ferroptosis inducer (erastin), promotion inhibitor (Fer-1). Taken together, lens epithelial cells treated with restrained through regulating ferroptosis, suggesting that might be a target for cataract treatment.

Язык: Английский

Процитировано

0
Proteomic Analysis of Retinas in a Rat Model of High-Fat Diet-Induced Type 2 Diabetes: Implications of Interventional Targets for Nonproliferative Diabetic Retinopathy DOI Creative Commons
Xinxin Pang,

Siqiong Bai,

Zhinan Feng

и другие.

Drug Design Development and Therapy, Год журнала: 2025, Номер Volume 19, С. 2979 - 2999

Опубликована: Апрель 1, 2025

This study aimed to establish a high-fat diet (HFD)-induced rat model of type 2 diabetes mellitus (T2DM) and employed tandem mass tag (TMT) proteomics search for novel interventional targets nonproliferative diabetic retinopathy (NPDR). Six-week-old male Sprague-Dawley rats were randomly divided into T2DM group fed HFD normal (NOR group) chow. After 6 w, the was confirmed have impaired glucose tolerance intraperitoneally injected with single small dose streptozotocin (STZ, 30 mg/kg), blood levels monitored. The maintained another an Evans blue assay dark-adapted electroretinogram (ERG) conducted. Rat retinas collected morphology analysis, TMT Western blotting. expression patterns selected differentially expressed proteins (DEPs) validated in via blotting aqueous humor from NPDR patients slot 12-w STZ injection, presented typical symptoms T2DM. pathological features NPDR, including compromised scotopic ERGs, thinning retinal layers, increased apoptosis vascular leakage retina. Proteomic analysis identified DEPs revealed profound dyslipidemia retinas. significant upregulation FABP3, TINAGL1, COL4A3 by In HFD-induced that is consistent natural history bioinformatics analyses as 3 key upregulated first time. These findings are supported technical clinical validations provide intervention.

Язык: Английский

Процитировано

0