Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Окт. 23, 2024
Abstract
Using
plant
extracts
in
the
green
synthesis
of
nanoparticles
has
become
an
environmentally
acceptable
approach.
In
our
study,
copper
oxide
(CuO
NPs)
were
synthesized
using
ethanolic
Azadirachta
indica
and
Simmondsia
chinensis
.
CuO
NP
formation
was
confirmed
by
change
color
UV‒visible
spectroscopy
NPs
peaked
at
a
wavelength
344
nm).
TEM
images
semispherical
shape
NPs,
with
particle
sizes
ranging
from
30.9
to
10.7
nm.
The
antibacterial
activity
these
evaluated
agar
diffusion
method
against
clinical
isolates,
including
methicillin-resistant
Staphylococcus
aureus
(MRSA),
Escherichia
coli
,
Pseudomonas
aeruginosa
Acinetobacter
spp.,
Klebsiella
pneumoniae
Stenotrophomonas
maltophilia
minimum
inhibitory
concentration
(MIC)
ranged
62.5
125
µg/ml.
contrast,
antioxidant
antibiofilm
31.1
92.2%
125–500
µg/ml
62.2–95%,
respectively,
–62.5
Our
results
that
had
IC50s
383.41
±
3.4
402.73
1.86
250
µg/mL
HBF4
cell
line.
Molecular
docking
studies
suggested
penicillin-binding
protein
4
(PBP4)
beta-lactamase
proteins
(OXA-48)
strongly
bind
S.
K.
NPs.
study
confirms
promising
use
treating
pathogenic
bacteria
could
be
possible
alternative
antibiotics.
This
supports
pharmaceutical
healthcare
sectors
Egypt
worldwide.
Heliyon,
Год журнала:
2024,
Номер
10(11), С. e32333 - e32333
Опубликована: Июнь 1, 2024
In
recent
decades,
biosynthesis
of
metal
and
(or)
oxide
nanoparticles
using
microbes
is
accepted
as
one
the
most
sustainable,
cost-effective,
robust,
green
processes
it
does
not
encompass
usage
largely
hazardous
chemicals.
Accordingly,
numerous
simple,
inexpensive,
environmentally
friendly
approaches
for
silver
(AgNPs)
were
reported
avoiding
conventional
(chemical)
methods.
This
comprehensive
review
detailed
an
advance
made
in
years
microbes-mediated
AgNPs
evaluation
their
antimicrobial
activities
covering
literature
from
2015-till
date.
It
also
aimed
at
elaborating
possible
effect
different
phytochemicals,
concentrations,
extraction
temperature,
solvent,
pH,
reaction
time,
concentration
precursor
on
shape,
size,
stability
synthesized
AgNPs.
addition,
while
trying
to
understand
against
targeted
pathogenic
probable
mechanism
interaction
produced
with
cell
wall
that
led
cell's
reputed
death
have
been
detailed.
Lastly,
this
shape
size-dependent
enhanced
by
synergetic
known
commercially
available
antibiotic
drugs.
Green Processing and Synthesis,
Год журнала:
2024,
Номер
13(1)
Опубликована: Янв. 1, 2024
Abstract
Some
of
the
significant
globally
prevalent
vector-borne
illnesses
are
caused
by
Culex
pipiens
.
Synthetic
pesticides
have
been
widely
utilized
to
eradicate
C.
,
which
has
led
a
number
health
risks
for
people,
insect
resistance,
and
environmental
contamination.
Alternative
strategies
therefore
vitally
needed.
In
current
investigation,
Trichoderma
viride
fungal
culture
filtrate
was
used
create
selenium
silver
nanoparticles
(SeNPs
AgNPs,
respectively)
test
them
on
larvae
in
their
fourth
instar
stage.
The
death
rate
increased
significantly
when
SeNP
AgNP
concentrations
increased,
according
results.
SeNPs
AgNPs
affected
developmental
detoxification
enzymes
at
24
h
after
being
treated
with
sublethal
concentration
tested
NPs.
As
result
insecticidal
effect
larvae,
considered
effective
promising
larvicidal
agents.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Янв. 3, 2024
Abstract
Recently,
multi-drug
resistant
(MDR)
bacteria
are
responsible
for
a
large
number
of
infectious
diseases
that
can
be
life-threatening.
Globally,
new
approaches
targeted
to
solve
this
essential
issue.
This
study
aims
discover
novel
antibiotic
alternatives
by
using
the
whole
components
biofilm
layer
as
macromolecule
synthesize
silver
nanoparticles
(AgNPs)
promising
agent
against
MDR.
In
particular,
biosynthesized
biofilm-AgNPs
were
characterized
UV-Vis
spectroscopy,
electron
microscopes,
Energy
Dispersive
X-ray
(EDX),
zeta
sizer
and
potential
while
their
effect
on
bacterial
strains
normal
cell
lines
was
identified.
Accordingly,
have
lavender-colored
solution,
spherical
shape,
with
size
range
20–60
nm.
Notably,
they
inhibitory
effects
when
used
various
concentrations
ranging
between
12.5
25
µg/mL.
addition,
an
effective
synergistic
combined
phage
ZCSE9
inhibit
kill
Salmonella
enterica
concentration
3.1
conclusion,
work
presents
biosynthesis
preparation
AgNPs
antibacterial
purposes
reduce
possible
toxicity
reducing
MICs
ZCSE9.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Окт. 23, 2024
Abstract
Using
plant
extracts
in
the
green
synthesis
of
nanoparticles
has
become
an
environmentally
acceptable
approach.
In
our
study,
copper
oxide
(CuO
NPs)
were
synthesized
using
ethanolic
Azadirachta
indica
and
Simmondsia
chinensis
.
CuO
NP
formation
was
confirmed
by
change
color
UV‒visible
spectroscopy
NPs
peaked
at
a
wavelength
344
nm).
TEM
images
semispherical
shape
NPs,
with
particle
sizes
ranging
from
30.9
to
10.7
nm.
The
antibacterial
activity
these
evaluated
agar
diffusion
method
against
clinical
isolates,
including
methicillin-resistant
Staphylococcus
aureus
(MRSA),
Escherichia
coli
,
Pseudomonas
aeruginosa
Acinetobacter
spp.,
Klebsiella
pneumoniae
Stenotrophomonas
maltophilia
minimum
inhibitory
concentration
(MIC)
ranged
62.5
125
µg/ml.
contrast,
antioxidant
antibiofilm
31.1
92.2%
125–500
µg/ml
62.2–95%,
respectively,
–62.5
Our
results
that
had
IC50s
383.41
±
3.4
402.73
1.86
250
µg/mL
HBF4
cell
line.
Molecular
docking
studies
suggested
penicillin-binding
protein
4
(PBP4)
beta-lactamase
proteins
(OXA-48)
strongly
bind
S.
K.
NPs.
study
confirms
promising
use
treating
pathogenic
bacteria
could
be
possible
alternative
antibiotics.
This
supports
pharmaceutical
healthcare
sectors
Egypt
worldwide.
