Impact of ZnO nanoparticles on MagA gene expression in Klebsiella pneumoniae isolated from diabetic foot ulcers DOI Open Access

Mustafa Muhammed Jadooa,

Haider Turky Mousa Al-Mousawi,

Nadhim Mushtaq Hashim Al-bderee

и другие.

Asia-Pacific Journal of Molecular Biology and Biotechnology, Год журнала: 2024, Номер unknown, С. 248 - 256

Опубликована: Сен. 26, 2024

Diabetes mellitus (DM) is an accumulation of glucose in the blood. Diabetic divided into gestational diabetes, type 1 and 2. Complications diabetes include diabetic foot ulcers. The most important complication ulcers bacterial infection, which leads to gangrene when various types antibiotics fail prevent infections. To explore effect synthetic zinc oxide nanoparticles (ZnONPs) on expression MagA gene Klebsiella pneumoniae isolated from ulcer cases. ZnONPs were biosynthesized by Aspergillus niger characterized using scanning electron microscopy (SEM), Fourier transform infrared (FTIR), X-ray diffraction (XRD), UV-visible spectroscopy. inhibitory K. isolates was examined determining minimum concentration (MIC) ZnONPs. Besides, values before after exposure would be RT-qPCR, with 16SrRNA as reference gene. According SEM XRD results, are form nanocrystals have small spherical crystals average size 13.5 nm. highest optical density synthesized obtained at 390 exhibit a strong inhibition zone against corresponding 125 μg/mL. fold ranged 0.042 0.118. research reveals that antibacterial potential affect strains. results present study indicate can indeed used treatment infectious bacteria.

Язык: Английский

Fabrication of chitosan-coated ciprofloxacin drug nanocomposite for combating antibacterial and respiratory infection leading to sepsis DOI
Haoguang Li,

Zhiguo Su

Journal of materials research/Pratt's guide to venture capital sources, Год журнала: 2025, Номер unknown

Опубликована: Янв. 10, 2025

Язык: Английский

Процитировано

0

Synthesis and Characterization of Silver Nanoparticles-Chitosan Beads as Antibacterial Agents DOI Creative Commons

Ayu Safirah,

Atik Rismawati,

Mohammad Alauhdin

и другие.

Jurnal Kimia Sains dan Aplikasi, Год журнала: 2025, Номер 28(1), С. 47 - 52

Опубликована: Янв. 30, 2025

One of the key innovations in silver nanoparticle (AgNPs) material development is formation AgNPs/chitosan beads, which exhibit enhanced properties and broader functionality compared to chitosan or AgNPs alone. In this study, beads were synthesized using glutaraldehyde as a binding agent sodium citrate reducing agent—both are safe non-toxic, enabling applications. The characterized assess their chemical physical properties, including functional groups, morphology, crystallinity. Characterization results confirmed successful cross-linking by glutaraldehyde, enhancing material’s strength. appeared white, while brownish, with an average bead size 1.99 mm. X-ray diffraction analysis revealed that exhibited face-centered cubic crystal structure. Antibacterial testing demonstrated superior antibacterial activity attributed release Ag+ ions from matrix.

Язык: Английский

Процитировано

0

Construction and Characterization of Polyethylene Glycol/Sodium Alginate Hydrogel Loaded With Zirconia Nanoparticles: Potential Antibacterial and Antibiofilm Agent to Inhibit Dental Caries In Vitro and In Vivo DOI Creative Commons

Layth L. Hamid,

Roqaya S. Zakir,

Nur indah noviyanti Rahman

и другие.

Microscopy Research and Technique, Год журнала: 2025, Номер unknown

Опубликована: Март 21, 2025

Biofilm formation on tooth surfaces is a primary contributor to dental caries and periodontal diseases. Streptococcus mutans recognized for its role in biofilm production, significantly influencing the development of caries. Key virulence factors associated with S. biofilms include acid tolerance, synthesis exopolysaccharides (EPS). This study presents novel approach by focusing loading biosynthesized zirconia nanoparticles (ZrO2 NPs) onto polyethylene glycol/sodium alginate (PEG/SA) hydrogel nanocomposite, evaluating their effects biofilm-forming ability both vivo vitro. ZrO2 NPs were using Citrus aurantifolia (C. aurantifolia) extract incorporated into PEG/SA beads through sol-gel process. The PEG/SA/ZrO2 nanocomposite was confirmed diverse analyzes, including UV-visible spectroscopy, particle size measurement, morphology examination, spectral analysis, thermal gravimetric analysis (TGA) hemolysis studies. average approximately 26 nm, while exhibited highly porous, sheet-like surface structure. In vitro results demonstrated inhibition zones 30 28 mm against mutans, respectively, minimum inhibitory concentration (MIC) 12.5 mg/mL. growth curve indicated complete decline an 87% reduction when treated beads. SEM revealed that cells appeared lysed or crumpled, losing characteristic coccal shape after exposure Additionally, images effective prevention attachment teeth encapsulated hydrogel, altering mature developed treatment. Finally, incorporation hydrogels demonstrates significant potential as strategy inhibiting may serve promising topical agent reducing Further studies could explore long-term efficacy clinical applications this oral health care.

Язык: Английский

Процитировано

0

Truffle mediated preparation of bacterial culture medium and mycosynthesis of titanium oxide nanoparticles loaded with polyvinyl alcohol/sodium alginate aerogel beads for antibacterial activity DOI

Layth L. Hamid,

Hanan Hamed,

Abdulbaset Mohammed Al-Fahdawi

и другие.

Journal of Sol-Gel Science and Technology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 14, 2024

Язык: Английский

Процитировано

3

Impact of ZnO nanoparticles on MagA gene expression in Klebsiella pneumoniae isolated from diabetic foot ulcers DOI Open Access

Mustafa Muhammed Jadooa,

Haider Turky Mousa Al-Mousawi,

Nadhim Mushtaq Hashim Al-bderee

и другие.

Asia-Pacific Journal of Molecular Biology and Biotechnology, Год журнала: 2024, Номер unknown, С. 248 - 256

Опубликована: Сен. 26, 2024

Diabetes mellitus (DM) is an accumulation of glucose in the blood. Diabetic divided into gestational diabetes, type 1 and 2. Complications diabetes include diabetic foot ulcers. The most important complication ulcers bacterial infection, which leads to gangrene when various types antibiotics fail prevent infections. To explore effect synthetic zinc oxide nanoparticles (ZnONPs) on expression MagA gene Klebsiella pneumoniae isolated from ulcer cases. ZnONPs were biosynthesized by Aspergillus niger characterized using scanning electron microscopy (SEM), Fourier transform infrared (FTIR), X-ray diffraction (XRD), UV-visible spectroscopy. inhibitory K. isolates was examined determining minimum concentration (MIC) ZnONPs. Besides, values before after exposure would be RT-qPCR, with 16SrRNA as reference gene. According SEM XRD results, are form nanocrystals have small spherical crystals average size 13.5 nm. highest optical density synthesized obtained at 390 exhibit a strong inhibition zone against corresponding 125 μg/mL. fold ranged 0.042 0.118. research reveals that antibacterial potential affect strains. results present study indicate can indeed used treatment infectious bacteria.

Язык: Английский

Процитировано

0