The value of ITGA and ITGB superfamily genes as biomarkers for the early diagnosis and prognosis of gliomas: A retrospective observational study based on the GEPIA and CGGA databases
NeuroMarkers.,
Год журнала:
2025,
Номер
unknown, С. 100039 - 100039
Опубликована: Фев. 1, 2025
Язык: Английский
Tumor Microenvironment On‐A‐Chip and Single‐Cell Analysis Reveal Synergistic Stromal–Immune Crosstalk on Breast Cancer Progression
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 8, 2025
Solid
tumors
develop
within
a
complex
environment
called
the
tumor
microenvironment
(TME),
which
is
sculpted
by
presence
of
other
cells,
such
as
cancer-associated
fibroblasts
(CAFs)
and
immune
cells
like
macrophages
(Mφs).
Despite
orchestrate
tumor-supportive
through
intricate
interaction
with
components
TME.
However,
specific
mechanism
this
intercellular
dialogue
regulated
not
fully
understood.
To
that
end,
development
an
organotypic
3D
breast
TME-on-a-chip
(TMEC)
model,
integrated
single-cell
RNA
sequencing
analysis,
reported
to
mechanistically
evaluate
progression
triple-negative
cancer
(TNBC)
in
patient-derived
CAFs
Mφs.
Extensive
functional
assays,
including
invasion
morphometric
characterization,
reveal
synergistic
influence
Mφs
on
cells.
Furthermore,
gene
expression
pathway
enrichment
analyses
identify
involvement
KYNU
gene,
suggesting
potential
evasion
kynurenine
pathway.
Lastly,
pharmacological
targeting
identified
investigated.
Язык: Английский
Single-cell RNA sequencing explores the evolution of the ecosystem from leukoplakia to head and neck squamous cell carcinoma
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Апрель 6, 2024
Abstract
It
has
been
found
that
progression
from
leukoplakia
to
head
and
neck
squamous
cell
carcinoma
(HNSCC)
is
a
long-term
process
may
involve
changes
in
the
multicellular
ecosystem.
We
acquired
scRNA-seq
samples
information
gene
expression
omnibus
UCSC
Xena
database.
The
BEAM
function
was
used
construct
pseudotime
trajectory
analyze
differentially
expressed
genes
different
branches.
ssGSEA
method
explore
correlation
between
each
subgroup
survival
time,
obtained
related
prognosis.
During
HNSCC,
we
several
prognostic
subgroups,
such
as
AURKB
+
epithelial
cells,
SFRP1
fibroblasts,
SLC7A8
macrophages,
FCER1A
CD1C
dendritic
TRGC2
NK/T
cells.
All
subgroups
had
two
fates,
one
tending
proliferation,
migration,
enhancement
of
angiogenesis
capacity,
other
inflammatory
immune
response,
leukocyte
chemotaxis,
T
activation.
Tumor-promoting
CD163
CD209
were
highly
myeloid
depletion
marker
TIGIT,
LAG3
Our
study
provide
reference
for
molecular
mechanism
HNSCC
theoretical
basis
development
new
therapeutic
strategies.
Язык: Английский
Osteopontin Regulates Treg Cell Stability and Function with Implications for Anti-Tumor Immunity and Autoimmunity
Cancers,
Год журнала:
2024,
Номер
16(17), С. 2952 - 2952
Опубликована: Авг. 24, 2024
Foxp3-expressing
regulatory
T
(Treg)
cells
represent
the
most
highly
immunosuppressive
cell
in
tumor
microenvironment
(TME)
that
halts
effective
anti-tumor
immunity.
Osteopontin
(Opn),
an
extracellular
matrix
(ECM)
glycophosphoprotein,
plays
key
roles
many
types
of
immune-related
diseases
and
is
associated
with
cancer
aggressiveness
when
expressed
by
cells.
However,
its
role
Foxp3Treg
heterogeneity,
function,
stability
TME
poorly
defined.
We
generated
mice
a
Foxp3-specific
deletion
Opn
assessed
ability
Opn-deficient
Tregs
to
suppress
inflammation.
As
these
aged,
they
developed
scurfy-like
syndrome
characterized
aberrant
excessive
activation
effector
evaluated
further
confirmed
reduced
suppressive
capacity
vivo
suppression
assay
colitis.
also
found
Foxp3
Язык: Английский