DDX21 Controls Cell Cycle Progression and Autophagy in Pancreatic Cancer Cells DOI Open Access

Adriana Leccese,

Veronica Ruta, Valentina Panzeri

и другие.

Cancers, Год журнала: 2025, Номер 17(4), С. 570 - 570

Опубликована: Фев. 7, 2025

Background: Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal cancer. Late diagnosis and acquisition of chemoresistance contribute to its dismal prognosis. While surgical resection improved the clinical outcome patients, only ~20% them are eligible due advanced disease at diagnosis. Thus, development new therapeutic approaches master priority for an management this The helicase DDX21 was proposed as prognostic marker in several tumors, including PDAC. Methods:DDX21 expression evaluated PDAC samples cell lines; RNA sequencing bioinformatics analyses DDX21-depleted PANC-1 silenced cells; functional autophagy, cycle proliferation. Results: expressed higher levels liver metastasis patients. Transcriptomics cells revealed enrichment genes involved autophagy progression. inactivation by interference enhanced basal autophagic flux altered reducing rate G1-S transition. Coherently, proliferation clonogenic activity significantly reduced. Conclusions: Our results support oncogenic role uncover regulation autophagy.

Язык: Английский

Novel Insights into T-Cell Exhaustion and Cancer Biomarkers in PDAC Using Single Cell RNA Sequencing DOI Creative Commons
Muhammad Saleem,

Hammad Ali Sajid,

Muhammad Imran Shabbir

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Abstract One of the aggressive and lethal cancers, Pancreatic ductal adenocarcinoma (PDAC) is characterised by poor prognosis resistance to conventional treatments. Moreover, tumor immune microenvironment (TIME) plays a crucial role in progression therapeutic PDAC. It associated with T-cells exhaustion, leading progressive loss T-cell functions impaired ability kill cells. Therefore, this study employed single cell RNA sequencing (scRNA-seq) analysis identifying upregulated genes T-cells; cancer cells two groups (“cancer cells_vs_all-PDAC” “cancer-PDAC_vs_all-normal”). Common unique markers from both were identified. The Reactome pathways selected; while implicated those used perform PPI analysis, revealing hub-genes T-cells. gene expression validation was performed using GEPIA2 TISCH2, overall survival GEPIA2. Conclusively, unravelled 16 novel providing groundwork for future research into landscape PDAC, particularly exhaustion. However, further clinical studies are needed validate these as potential targets PDAC patients. Graphical abstract

Язык: Английский

Процитировано

0
DDX21 Controls Cell Cycle Progression and Autophagy in Pancreatic Cancer Cells DOI Open Access

Adriana Leccese,

Veronica Ruta, Valentina Panzeri

и другие.

Cancers, Год журнала: 2025, Номер 17(4), С. 570 - 570

Опубликована: Фев. 7, 2025

Background: Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal cancer. Late diagnosis and acquisition of chemoresistance contribute to its dismal prognosis. While surgical resection improved the clinical outcome patients, only ~20% them are eligible due advanced disease at diagnosis. Thus, development new therapeutic approaches master priority for an management this The helicase DDX21 was proposed as prognostic marker in several tumors, including PDAC. Methods:DDX21 expression evaluated PDAC samples cell lines; RNA sequencing bioinformatics analyses DDX21-depleted PANC-1 silenced cells; functional autophagy, cycle proliferation. Results: expressed higher levels liver metastasis patients. Transcriptomics cells revealed enrichment genes involved autophagy progression. inactivation by interference enhanced basal autophagic flux altered reducing rate G1-S transition. Coherently, proliferation clonogenic activity significantly reduced. Conclusions: Our results support oncogenic role uncover regulation autophagy.

Язык: Английский

Процитировано

0