Identification of novel cytoskeleton protein involved in spermatogenic cells and sertoli cells of non-obstructive azoospermia based on microarray and bioinformatics analysis

BMC Medical Genomics, Год журнала: 2025, Номер 18(1)

Опубликована: Янв. 25, 2025

Язык: Английский

Reduction of Prostate Cancer Risk: Role of Frequent Ejaculation-Associated Mechanisms

Cancers, Год журнала: 2025, Номер 17(5), С. 843 - 843

Опубликована: Фев. 28, 2025

Prostate cancer (PCa) accounts for roughly 15% of diagnosed cancers among men, with disease incidence increasing worldwide. Age, family history and ethnicity, diet, physical activity, chemoprevention all play a role in reducing PCa risk. The prostate is an exocrine gland that characterized by its multi-functionality, being involved reproductive aspects such as male ejaculation orgasmic ecstasy, well playing key roles the regulation local systemic concentrations 5α-dihydrotestosterone. increase androgen receptors at ventral first elevated response induced copulation. growth function mediated androgen-dependent mechanism. Binding 5-DHT to (AR) results formation 5α-DHT:AR complex. interaction complex specific DNA enhancer element androgen-regulated genes leads androgen-specific target maintain homeostasis. Consequently, may significant reduction Thus, frequent absence risky sexual behavior possible approach prevention PCa. In this review, we provide insight into mechanisms regulating impact on

Язык: Английский

Integrating Microarray Data and Single-Cell RNA-Seq Reveals Key Gene Involved in Spermatogonia Stem Cell Aging

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(21), С. 11653 - 11653

Опубликована: Окт. 30, 2024

The in vitro generation of spermatogonial stem cells (SSCs) from embryonic (ESCs) offers a viable approach for addressing male infertility. A multitude molecules participate this intricate process, which requires additional elucidation. Despite the decline SSCs aged testes, are deemed immortal since they can multiply three years with repeated transplantation. Nonetheless, examination aging is challenging due to limited quantity and absence precise indicators. Using microarray, we assessed genome-wide transcripts (about 55,000 transcripts) fibroblasts SSCs. WGCNA was then used look SSC-specific transcription factors (TFs) hub genes based on ATAC-seq, DNase-seq, RNA-seq, microarray data GEO databases as well gene expression (RNA-seq data). analysis human cases different revealed that 6 were upregulated, 23 downregulated compared normal case relation genes. To reach these results, online assessments Enrich Shiny GO, STRING, Cytoscape forecast molecular functional connections proteins before identifying master routes. biological process function keywords cell-matrix adhesion, telomerase activity, telomere cap complex shown be significantly altered upregulated differentially expressed (DEGs) by enrichment analysis. According our preliminary research, cell-specific TFs TF-mediated GRNs involved creation In order maximize induction efficiency ESC differentiation into vitro, important identified, sophisticated network regulation proposed. interact may able shine light pathophysiologies infertility aberrant germ cells.

Язык: Английский