Materials Today Bio, Год журнала: 2025, Номер unknown, С. 101667 - 101667
Опубликована: Март 1, 2025
Язык: Английский
The EPMA Journal, Год журнала: 2023, Номер 14(1), С. 21 - 42
Опубликована: Фев. 13, 2023
Abstract Proliferative diabetic retinopathy (PDR) the sequel of (DR), a frequent complication diabetes mellitus (DM), is leading cause blindness in working-age population. The current screening process for DR risk not sufficiently effective such that often disease undetected until irreversible damage occurs. Diabetes-associated small vessel and neuroretinal changes create vicious cycle resulting conversion into PDR with characteristic ocular attributes including excessive mitochondrial retinal cell damage, chronic inflammation, neovascularisation, reduced visual field. considered an independent predictor other severe complications as ischemic stroke. A “domino effect” highly cascading DM which early indicator impaired molecular signaling. Mitochondrial health control clinically relevant management, multi-omic tear fluid analysis can be instrumental prognosis prediction. Altered metabolic pathways bioenergetics, microvascular deficits disease, tissue remodelling are focus this article evidence-based targets predictive approach to develop diagnosis treatment algorithms tailored individual cost-effective prevention by implementing paradigm shift from reactive medicine predictive, preventive, personalized (PPPM) primary secondary care management.
Язык: Английский
Процитировано
182
Molecules, Год журнала: 2022, Номер 27(8), С. 2411 - 2411
Опубликована: Апрель 8, 2022
Mass Spectrometry (MS) allows the analysis of proteins and peptides through a variety methods, such as Electrospray Ionization-Mass (ESI-MS) or Matrix-Assisted Laser Desorption (MALDI-MS). These methods allow identification mass protein peptide intact molecules peptide-mass fingerprinting generated upon enzymatic digestion. Tandem spectrometry (MS/MS) fragmentation to determine amino acid sequence (top-down middle-down proteomics) (bottom-up proteomics). Furthermore, tandem also post-translational modifications (PTMs) peptides. Here, we discuss application MS/MS in biomedical research, indicating specific examples for their PTMs relevant biomarkers diagnostic therapy.
Язык: Английский
Процитировано
71
Chemical Reviews, Год журнала: 2023, Номер 123(19), С. 11488 - 11558
Опубликована: Сен. 25, 2023
The eye contains a complex network of physiological information and biomarkers for monitoring disease managing health, ocular devices can be used to effectively perform point-of-care diagnosis management. This comprehensive review describes the target various diseases, including ophthalmic metabolic neurological based on anatomical background eye. also includes recent technologies utilized in eye-wearable medical latest trends wearable devices, specifically smart contact lenses purpose After introducing other such as retinal prosthesis, we further discuss current challenges potential possibilities lenses.
Язык: Английский
Процитировано
59
Journal of Advanced Research, Год журнала: 2023, Номер 54, С. 271 - 292
Опубликована: Фев. 14, 2023
Triple-negative breast cancer (TNBC) is a heterogeneous, aggressive phenotype of with associated chemoresistance. The development chemo- or radioresistance could be attributed to diverse tumor microenvironments, overexpression membrane proteins (transporters), epigenetic changes, and alteration the cell signaling pathways/genes stem cells (CSCs). Due heterogeneous nature TNBC, therapeutic response existing modalities offers limited scope thus results in reccurance after therapy. To establish landmark efficacy, number novel have been proposed. In addition, reversal resistance that developed during treatment may altered by employing appropriate modalities. This review aims discuss plethora investigations carried out, which will help readers understand make an choice therapy directed toward complete elimination TNBC. manuscript addresses major contributory factors from microenvironment are responsible for chemoresistance poor prognosis. cellular events molecular mechanism-based interventions explained detail. Inhibition ABC transporters, pathways CSCs, modification promising this regard. TNBC progression, invasion, metastasis recurrence can also inhibited blocking multiple pathways, targeting specific receptors/epigenetic targets, disrupting bioenergetics generating reactive oxygen species (ROS).
Язык: Английский
Процитировано
57
The EPMA Journal, Год журнала: 2024, Номер 15(1), С. 1 - 23
Опубликована: Фев. 27, 2024
Worldwide stroke is the second leading cause of death and third disability combined. The estimated global economic burden by over US$891 billion per year. Within three decades (1990-2019), incidence increased 70%, deaths 43%, prevalence 102%, DALYs 143%. Of 100 million people affected stroke, about 76% are ischemic (IS) patients recorded worldwide. Contextually, moves into particular focus multi-professional groups including researchers, healthcare industry, economists, policy-makers. Risk factors demonstrate sufficient space for cost-effective prevention interventions in primary (suboptimal health) secondary (clinically manifested collateral disorders contributing to risks) care. These risks interrelated. For example, sedentary lifestyle toxic environment both mitochondrial stress, systemic low-grade inflammation accelerated ageing; inflammageing a associated with ageing poor outcomes. Stress overload, decreased bioenergetics hypomagnesaemia vasospasm lesions heart brain all age teenagers. Imbalanced dietary patterns folate but rich red processed meat, refined grains, sugary beverages hyperhomocysteinaemia, inflammation, small vessel disease, IS risks. Ongoing 3PM research towards vulnerable population promoted European Association Predictive, Preventive Personalised Medicine (EPMA) demonstrates promising results holistic patient-friendly non-invasive approach utilising tear fluid-based health risk assessment, mitochondria as vital biosensor AI-based data interpretation reported here EPMA expert group. Collected that IS-relevant corresponding molecular pathways examples, there an evident overlap between involved diabetic retinopathy early indicator patients. Just exemplify some them such 5-aminolevulinic acid/pathway, which also characteristic altered mitophagy patterns, insomnia, stress regulation modulation microbiota-gut-brain crosstalk. Further, ceramides considered mediators oxidative cardiometabolic negatively affecting respiratory chain function fission/fusion activity, sleep-wake behaviour, vascular stiffness remodelling. Xanthine/pathway homeostasis stress-driven anxiety-like behaviour well mechanisms arterial stiffness. In order assess individual risks, application machine learning (AI tool) essential accurate performed multiparametric analysis. Aspects presented paper include needs young populations elderly, personalised assessment care, cost-efficacy, innovative technologies screening programmes, advanced education measures professionals general population-all pillars paradigm change from reactive medical services overall management EPMA.
Язык: Английский
Процитировано
22
The EPMA Journal, Год журнала: 2022, Номер 13(2), С. 315 - 334
Опубликована: Апрель 14, 2022
Breast cancer incidence is actually the highest one among all cancers. Overall breast management associated with challenges considering risk assessment and predictive diagnostics, targeted prevention of metastatic disease, appropriate treatment options, cost-effectiveness approaches applied. Accumulated research evidence indicates promising anti-cancer effects phytochemicals protecting cells against malignant transformation, inhibiting carcinogenesis spread, supporting immune system increasing effectiveness conventional therapies, others. Molecular sub-/cellular mechanisms are highly complex affecting several pathways considered potent targets for advanced diagnostics cost-effective treatments. Demonstrated affects, therefore, clinically relevant improving individual outcomes might be applicable to primary (protection initial development), secondary potential disease tertiary (towards cascading complications) care. However, a detailed data analysis essential adapt algorithms individuals' patients' needs. Consequently, concepts patient stratification, prevention, treatments tailored individualized profile instrumental application natural substances improve overall benefiting affected individuals society at large.
Язык: Английский
Процитировано
54
The EPMA Journal, Год журнала: 2022, Номер 13(1), С. 1 - 7
Опубликована: Март 1, 2022
According to GLOBOCAN, about 1.41 million new prostate cancer (PCa) cases were registered in the year 2020 globally. The corresponding socio-economic burden is enormous. Anti-cancer mRNA-based therapy a promising approach, principle of which currently applied for anti-COVID-19 vaccination, undergoing detailed investigation populations considering its short- and long-term effectiveness potential side effects. Pragmatically considered, it will take years or even decades make mRNA working any type cancers, if possible, individual malignancy sub-types are many specifically PCa. Actually, costs treating PCa increasing more rapidly than those other cancer. trend has be reversed now, not couple years. In general, two main components making reactive (management clinically manifested disease) treatment particularly expensive. On one hand, incidence disease metastatic as subtype. To this end, rates young middle-aged male sub-populations should taken into account contributor potentially developed later on life. patient stratification differentiate between non-metastatic (no need an extensive costly treatment) aggressive subtypes requiring personalised algorithms challenging. Considering current statistics, becomes obvious that medicine got at limit management. Multi-professional expertise unavoidable create implement anti-PCa programmes population. our strategic paper, we exemplify challenging management by providing expert recommendations primary (health risk assessment), secondary (prediction prevention PCa) tertiary (making palliative care chronic framework predictive, preventive medicine.
Язык: Английский
Процитировано
40
Journal of Advanced Research, Год журнала: 2023, Номер 55, С. 103 - 118
Опубликована: Март 4, 2023
Cancer management faces multiple obstacles, including resistance to current therapeutic approaches. In the face of challenging microenvironments, cancer cells adapt metabolically maintain their supply energy and precursor molecules for biosynthesis thus sustain rapid proliferation tumor growth. Among various metabolic adaptations observed in cells, altered glucose metabolism is most widely studied. The aberrant glycolytic modification has been associated with cell division, growth, progression, drug resistance. higher rates glycolysis as a hallmark modulated by transcription factor hypoxia inducible 1 alpha (HIF-1α), downstream target PI3K/Akt signaling, deregulated pathway cancer. We provide detailed overview current, primarily experimental, evidence on potential effectiveness flavonoids combat glycolysis-induced conventional targeted therapies. manuscript focuses reducing via affecting PI3K/Akt, HIF-1α (as critical that regulated pathway), key mediators PI3K/Akt/HIF-1α signaling (glucose transporters enzymes). working hypothesis proposes – an attractive application mitigate Phytochemicals represent source promising substances applicable primary, secondary, tertiary care. However, accurate patient stratification individualized profiling crucial steps paradigm shift from reactive predictive, preventive, personalized medicine (PPPM / 3PM). article focused targeting molecular patterns natural provides evidence-based recommendations 3PM relevant implementation.
Язык: Английский
Процитировано
29
Advances in clinical chemistry, Год журнала: 2024, Номер unknown, С. 69 - 115
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
9
Military Medical Research, Год журнала: 2022, Номер 9(1)
Опубликована: Июнь 17, 2022
Abstract Background Due to the outbreak and rapid spread of coronavirus disease 2019 (COVID-19), more than 160 million patients have become convalescents worldwide date. Significant alterations occurred in gut oral microbiome metabonomics with COVID-19. However, it is unknown whether their characteristics return normal after 1-year recovery. Methods We recruited 35 confirmed provide specimens at discharge one year later, as well healthy controls. A total 497 samples were prospectively collected, including 219 tongue-coating, 129 stool 149 plasma samples. Tongue-coating subjected 16S rRNA sequencing, untargeted metabolomics testing. Results The restored a large extent but did not completely normal. In recovery process, microbial diversity gradually increased. Butyric acid-producing microbes Bifidobacterium increased, whereas lipopolysaccharide-producing decreased. addition, sphingosine-1-phosphate, which closely related inflammatory factor storm COVID-19, increased significantly during process. Moreover, predictive models established based on metabolites time reached high efficacy predicting neutralizing antibody levels later. Conclusions This study first characterize key process identified, provided new treatment ideas for accelerating And afford insights situation benefited affected individuals healthcare.
Язык: Английский
Процитировано
36