PLoS ONE,
Год журнала:
2024,
Номер
19(4), С. e0300115 - e0300115
Опубликована: Апрель 25, 2024
The
biogenic
approach
for
the
synthesis
of
metal
nanoparticles
provides
an
efficient
eco-friendly
alternative
to
chemical
synthesis.
This
study
presents
a
novel
route
biosynthesis
silver
using
aqueous
sandalwood
(SW)
leaf
extract
as
source
reducing
and
capping
agents
under
mild,
room
temperature
conditions.
bioreduction
Ag
+
o
(SW-AgNPs)
was
accompanied
by
appearance
brown
color,
with
surface
plasmon
resonance
peak
at
340-360
nm.
SEM,
TEM
AFM
imaging
confirm
SW-AgNP’s
spherical
shape
size
range
10-32
DLS
indicates
hydrodynamic
49.53
nm
predominant
negative
Zeta
potential,
which
can
contribute
stability
nanoparticles.
FTIR
analysis
involvement
derived
polyphenols,
proteins
lipids
in
reduction
SW-AgNPs.
XRD
determines
face-centered-cubic
crystalline
structure
SW-AgNPs,
is
key
factor
affecting
biological
functions
cell
culture
methodologies
evaluate
effects
SW-AgNPs
on
proliferating
cells
originating
from
plants
human
cancer.
Exposure
groundnut
calli
resulted
enhanced
proliferation
leading
over
70%
higher
biomass
control,
defense
enzyme
activities,
secretion
metabolites
implicated
biotic
stress
resistance
(Crotonyl
isothiocyanate,
Butyrolactone,
2-Hydroxy-gamma-butyrolactone,
Maltol)
plant
(dl-Threitol).
MTT
NRU
were
performed
determine
cytotoxicity
cervical
cancer
cells.
specifically
inhibited
lines
SiHa
(IC
50
–2.65
ppm)
CaSki
–9.49
ppm),
indicating
potential
use
treatment.
opposing
effect
(enhanced
proliferation)
(inhibition)
are
both
beneficial
point
safe
application
culture,
agriculture
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(5), С. 1934 - 1934
Опубликована: Фев. 24, 2025
Bacteria
in
natural
environments
often
encounter
high
concentrations
of
metal
ions,
leading
to
the
development
defense
mechanisms
such
as
chemical
reduction.
This
process
can
result
formation
nanostructures
(NS)
ranging
from
1-100
nm,
which
have
valuable
properties
for
various
applications,
including
virucidal
agents.
Currently,
metallic
NS
with
activity
are
used
disinfectants
and
surface
protection
products.
However,
their
production
mainly
relies
on
physical
methods,
complex,
toxic,
energy-intensive.
A
sustainable
alternative
is
biosynthesis
nanostructures.
Our
research
focuses
gold
(AuNS)
using
environmental
bacteria
proteins,
aim
exploring
potential
agents
destroy
influenza
virus.
We
screened
under
conditions
HAuCl4,
identifying
eight
microorganisms
capable
growing
concentrations.
Staphylococcus
haemolyticus
BNF01
showed
highest
resistance
Au(III)
reduction,
up
0.25
mM
HAuCl4.
Bioinformatic
analysis
revealed
five
proteins
Au(III)-reductase
activity,
were
cloned
expressed
Escherichia
coli.
These
reduced
form
AuNPs,
purified,
characterized
size,
shape,
charge,
tested
against
A,
showing
significant
effects,
likely
due
interactions
viral
proteins.
International Journal of Nanomedicine,
Год журнала:
2025,
Номер
Volume 20, С. 2645 - 2658
Опубликована: Март 1, 2025
The
potential
use
of
gold
nanoparticles
(AuNPs)
in
healthcare
research
has
increased
dramatically
recent
years,
owing
to
advancements
their
synthesis
techniques,
including
green
synthesis.
Although
Clerodendrum
trichotomum
is
a
popular
medicinal
plant
that
harbors
many
bioactive
phytochemicals
various
parts
the
world,
AuNPs
using
this
precious
still
under
investigation.
Therefore,
study
aimed
explore
from
C.
Thunberg
leaves
(CTT-AuNPs)
and
antibacterial
cytotoxic
properties.
were
synthesized
characterized
microscopic
spectroscopic
techniques.
A
serial
dilution
technique
was
used
determine
activity
CTT-AuNPs
against
two
bacterial
species,
Klebsiella
pneumoniae
Staphylococcus
aureus.
cytotoxicity
assay
carried
out
breast
cancer
cell
line
(MCF-7),
whereas
biocompatibility
test
performed
kidney
(293T).
Multiple
characterization
techniques
confirmed
successful
CTT-AuNPs.
average
size
19.1±2.2
nm.
results
revealed
showed
strong
S.
aureus
K.
pneumoniae,
which
further
live/dead
dual
staining
assay.
also
significantly
reduced
proliferation
MCF-7
cells
32.67%
at
120
µg/mL
after
24
hours
incubation.
These
green-synthesized
exhibited
excellent
cytobiocompatibility
with
293T
cells.
method
compared
chemically
AuNPs.
This
work
will
pave
way
for
production
biocompatible
can
be
different
disease
treatments.
