CO-ADMINISTRATION OF RESVERATROL RESCUED LEAD-INDUCED TOXICITY IN DROSOPHILA MELANOGASTER

Environmental Toxicology and Pharmacology, Год журнала: 2024, Номер 109, С. 104470 - 104470

Опубликована: Май 18, 2024

Язык: Английский

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Growth hormone releasing peptide-6 (GHRP-6) prevents doxorubicin-induced myocardial and extra-myocardial damages by activating prosurvival mechanisms

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Май 30, 2024

Introduction: Dilated cardiomyopathy (DCM) is a fatal myocardial condition with ventricular structural changes and functional deficits, leading to systolic dysfunction heart failure (HF). DCM frequent complication in oncologic patients receiving Doxorubicin (Dox). Dox highly cardiotoxic drug, whereas its damaging spectrum affects most of the organs by multiple pathogenic cascades. Experimentally reproduced DCM/HF through administrations has shed light on drivers cardiotoxicity. Growth hormone (GH) releasing peptide 6 (GHRP-6) GH secretagogue expanding promising cardioprotective pharmacological properties. Here we examined whether GHRP-6 administration concomitant prevented onset damages otherwise healthy rats. Methods: Myocardial were sequentially evaluated transthoracic echocardiography. Autopsy was conducted at end period when dilation established. Semiquantitative histopathologic study included other internal samples. tissue fragments also addressed for electron microscopy study, characterization transcriptional expression ratio between Bcl-2 Bax. Serum samples destined REDOX system balance assessment. Results discussion: parallel fibers consumption dilation, accounting an effective preservation LV function. attenuated extracardiac toxicity preserving epithelial integrity, inhibiting interstitial fibrosis, ultimately reducing morbidity mortality. Mechanistically, proved sustain cellular antioxidant defense, upregulate prosurvival gene Bcl-2, preserve cardiomyocyte mitochondrial integrity. These evidences contribute pave potential avenues clinical use Dox-treated subjects.

Язык: Английский

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Unveiling the Therapeutic Potential of Kelulut (Stingless Bee) Honey in Alzheimer’s Disease: Findings from a Rat Model Study

Antioxidants, Год журнала: 2024, Номер 13(8), С. 926 - 926

Опубликована: Июль 30, 2024

Alzheimer's disease (AD) poses a major worldwide health challenge because of its profound impact on cognitive abilities and overall well-being. Despite extensive research numerous clinical trials, therapeutic options remain limited. Our study aimed to investigate the potential Kelulut honey (KH) as novel agent for addressing multifactorial pathology AD. We tried evaluate disease-attenuating neuroprotective KH in intrahippocampally induced AD rat model by utilizing histochemistry enzyme-linked immunosorbent assay (ELISA) studies. A total 26 male Sprague Dawley rats weighing ~280-380 g were randomly divided into three groups: Control, AD-induced (Aβ), treated with (Aβ+KH). The latter two groups underwent stereotaxic surgery, where 6.25 µg amyloid β

Язык: Английский

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Immobilization of Superoxide Dismutase in Mesoporous Silica and its Applications in Strengthening the Lifespan and Healthspan of Caenorhabditis elegans

Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10

Опубликована: Июль 19, 2022

Senescence is a major inductive factor of aging-related diseases in connection with an accumulation reactive oxygen species (ROS). Therefore, it important to maintain ROS at appropriate level keep homeostasis organisms. Superoxide dismutase (SOD) vital enzyme defending against oxidative damage vivo . Because the defects direct application SOD and mimics, mounting delivery systems have been developed for efficient applications realize antioxidant treatment. Among these systems, mesoporous silica nanoparticles (MSNs) widely studied because various advantages such as desirable stability, low toxicity, adjustable particle sizes. Herein, was immobilized on MSNs using physical absorption strategy construct nanosystem SOD@MSN. The nematode Caenorhabditis elegans ( C. ) selected model organism subsequent anti-aging studies. research results suggested could not only be effectively internalized by but also protect external stress, thus extending lifespan healthspan SOD@MSN applied promising medicine therapeutics.

Язык: Английский

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Study on the Effects of Chinese Materia Medica Processing on the Hypoglycemic Activity and Chemical Composition of Anemarrhenae Rhizoma

Evidence-based Complementary and Alternative Medicine, Год журнала: 2021, Номер 2021, С. 1 - 11

Опубликована: Окт. 19, 2021

To compare the hypoglycemic effects of different extracts Anemarrhenae Rhizoma (AR) before and after being stir-baked with salt water on diabetic mice to detect contents 8 components in corresponding active parts simultaneously using UPLC-MS method, order screen better for diabetes clear material basis enhancing activity (SAR).Taking spontaneous type II db/db as models fasting blood glucose (FBG), oral tolerance test (OGTT), glycated hemoglobin or glycosylated (HbAlc), serum resistin (RESISTEIN), insulin (FINS), superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO) indicators, were evaluated. The chromatographic separation was performed a Waters BEH C18 (2.1 mm × 50 mm, 1.7 μm) column acetonitrile (B) 0.1% formic acid (A) mobile phases, flow rate 0.3 ml/min. temperature set 28°C, injection volume 10 μL. A mass spectrometer connected UPLC system via an electrospray ionization (ESI) interface. Full-scan data acquisition negative ion mode.In study pharmacodynamics, effect is than that ethanol extract more remarkable decoction. measured all have good linear relationship within their respective ranges (r ≥ 0.9990); average recovery rates are 98.86%-100.69%, RSD <2.90%. Compared raw Rhizoma, Timosaponin AIII, BII, BIII, Anemarrhenasaponin I, Ia, Mangiferin higher, changes AI AII not obvious, chemical composition obviously higher compared decoction.The processing stir-baking water, can increase compositions strengthen effect. site.

Язык: Английский

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