Study on the effects of Massa Medicata Fermentata with different formulations on the intestinal microbiota and enzyme activities in mice with spleen deficiency constipation

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 14

Опубликована: Янв. 7, 2025

This study aims to explore the therapeutic mechanism of Massa Medicata Fermentata (MMF) with different formulations on spleen deficiency constipation in mice by analyzing gastrointestinal hormones, D-xylose, intestinal microbiota, and enzyme activities. A model was established using an oral administration Sennae Folium decoction combined controlled diet water intake. After successful establishment, were treated MMF S1, S2, S3. Following intervention, serum samples from each group collected measure VIP, 5-HT, D-xylose. Additionally, small intestine contents analyzed for activity subjected 16S rRNA high-throughput sequencing. Mice showed significant decreases body weight fecal content. In contrast, CS2 CS3 groups returned normal levels, content also normal. The S2 S3 significantly increased protease sucrase enzymes levels compared CM group. Serum D-xylose reduced VIP but decreased groups. 5-HT CS1 significantly, returning showing increases. sequencing analysis revealed that all three effectively restored microbiota composition mice. LEfSe identified characteristic linked intervention upregulated chloroalkane chloroalkene degradation vibrio cholerae pathogenic cycle pathways Candidatus_Arthromitus Psychrobacter positive negative correlations respectively. alleviated symptoms modulating activities, restoring balance. as potential biomarkers treatment constipation. Different have mechanisms regulating through microbiota.

Язык: Английский

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Investigating the biological significance of the TCM principle “promoting urination to regulate bowel movements” through the influence of the intestinal microbiota and their metabolites on the renal-intestinal axis

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 14

Опубликована: Янв. 10, 2025

Treatment methods in traditional Chinese medicine (TCM) are foundational to their theoretical, methodological, formulaic, and pharmacological systems, significantly contributing syndrome differentiation therapy. The principle of "promoting urination regulate bowel movements" is a common therapeutic approach TCM. core concept the dispersion drainage water dampness, regulating relieve diarrhea," yet its scientific underpinning remains unclear. Modern medical treatment for watery diarrhea primarily focuses on electrolyte replenishment, as diuretics may lead dehydration other side effects. Some reports suggest that this TCM lacks validity. Microecology, an area associated with origins TCM, closely related development, diagnosis, diarrhea. renal-intestinal axis offers molecular biological basis examining pathological mechanisms, advancing targets such "treating intestine address kidney issues" highlighting interactions within "renal-intestinal microbiota-liquid metabolism" framework, thus providing endogenous mechanism support through kidney." An increasing number studies have shown intestinal microbiota metabolites, unique mediators, involved physiological changes body. Therefore, study explores relationship between fluid metabolism from perspective aiming elucidate mechanisms underlying clarify treating via axis. This research provides new insights microbiology.

Язык: Английский

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Effect of soluble dietary fiber extracted from Lentinula edodes (Berk.) Pegler on lipid metabolism and liver protection in mice on high-fat diet

Frontiers in Nutrition, Год журнала: 2025, Номер 12

Опубликована: Янв. 30, 2025

With the increasing annual production of Lentinula edodes , residues Lentinus are mass produced and wasted every year. In order to further explore added value effective utilization we studied lipid-lowering efficacy liver protective effect soluble dietary fiber in mice on high-fat diet. Project team from extracted fiber, its physicochemical properties, selected 30 male mice, randomly divided into normal group (N), high fat diet (F), add low dose (FL), medium (FM), (FH) five groups. After 4 weeks, assessed general state, organ conditions, status, blood parameters, expression hepatic lipid metabolism genes, mRNA levels key genes. The results showed that molecular weight is about 17.029 kDa, monosaccharides such as galactose, glucose mannitol connected by β-glycosidic bond. can effectively slow growth due diet, delay tissue lesions, reduce ALT, AST, ACP, LDL-C, TG, TV, FFA, SOD, GSH MDA, increase γ-GT, HDL-C CAT blood. decreased AMPKα SREBP-2 liver, increased PPARα, ACS, CPT1a, CYP7A1. It proved alleviate accumulation caused some extent, combat injury, oxidative stress pressure disorder provide an experimental basis for subsequent use reduction.

Язык: Английский

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Gut-brain axis mediated by intestinal content microbiota was associated with Zhishi Daozhi decoction on constipation

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Фев. 3, 2025

Background Constipation is a common digestive system disorder, which closely related to the intestinal flora. Zhishi Daozhi decoction (ZDD) traditional Chinese medicine prescription used treat constipation caused by indigestion. This study evaluate efficacy of ZDD in treating and elucidate underlying mechanism. Methods In this study, Kunming mice were administered high-protein diet (HFHPD) loperamide hydrochloride injections induce constipation. The then received varying doses (2.4, 4.7, 9.4 mg/kg) for seven days. Following sampling process, we measured fecal microbial activity. levels 5-hydroxytryptamine (5-HT), vasoactive peptide (VIP), aquaporin-3 (AQP3) quantified using enzyme-linked immunosorbent assay. Changes gut microbiota evaluated through 16S rRNA gene sequencing. Additionally, investigated correlation between specific features 5-HT, VIP, AQP3. Results surface model group (CMM) was rough dry. stool low-dose (CLD), medium-dose (CMD), high-dose (CHD) exhibited smoother texture, resembling that normal (CNM). 5-HT CMM significantly lower than CNM, CLD, CHD. VIP CMD other four groups, AQP3 showed decreasing trend. activity higher groups. Diversity analysis indicated CHD treatments more effective restoring structure. Potential pathogenic bacteria, including Clostridium , Aerococcus Jeotgalicoccus Staphylococcus enriched CMM. contrast, beneficial bacteria such as Faecalibacterium Bacillaceae Bacillus prevalent CMD, Correlation revealed Streptococcus Enterococcus positively correlated with while Succinivibrio negative 5-HT. Conclusions induced HFHPD disrupts structure microbiota. appears alleviate constipation, potentially mechanisms linked brain-gut axis its interaction Among treatment medium dose demonstrated most results.

Язык: Английский

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The intestinal microbiota modulates the visceral sensitivity involved in IBS induced by restraint combined with tail clustering

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Фев. 20, 2025

To compare three common stimuli that induce emotional stress to identify the optimal method for establishing an animal model aligns with clinical pathogenesis of irritable bowel syndrome (IBS) and explore gut microbiota mechanisms underlying IBS development. Thirty-six SPF-grade female Kunming mice were randomly divided into four groups: normal control (NC) group, restraint (BM) tail clamp (CTM) combined (BCTM) 9 in each group. The NC group was fed normally without any stimulation. BM subjected stress. CTM received intermittent BCTM underwent both stimulation time 1 hour, modeling duration 7 days. General behavioral changes observed. fecal water content measured calculated. pain threshold, gastric residue rate, small intestine propulsion serum levels short-chain fatty acids (SCFAs), serotonin (5-HT), interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-α) assessed. Histopathological analysis colon tissues performed. 16S rRNA high-throughput sequencing subsequently conducted. effects different on mouse symptoms, gastrointestinal motility, visceral hypersensitivity, inflammation levels, analyzed, correlation Compared BM, CTM, groups presented varying degrees hyperreactivity, accompanied by significantly reduced food intake markedly elevated inflammation, all which are indicative symptoms. Among them, most pronounced hyperreactivity irritability. had higher rates 5-HT a marked reduction tolerance. exhibited dysbiosis, diversity, structural composition, function microbial community. Specific bacterial taxa enriched their corresponding KEGG pathways also altered. Correlation revealed SCFAs positively correlated whereas rate negatively threshold. IL-10 TNF-α, TNF-α. In characteristic bacteria Acinetobacter Akkermansia 5-HT. 1. is superior among protocols successfully induces model. 2. may contribute development induced through regulation

Язык: Английский

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Targeting CD4+ T cells through gut microbiota: therapeutic potential of traditional Chinese medicine in inflammatory bowel disease

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Март 3, 2025

Inflammatory Bowel Disease (IBD) is an autoimmune disease characterized by chronic relapsing inflammation of the intestinal tract. Gut microbiota (GM) and CD4 + T cells are important in development IBD. A lot studies have shown that GM their metabolites like short-chain fatty acids, bile acids tryptophan can be involved differentiation through various mechanisms, which turn regulate immune homeostasis IBD patients. Therefore, regulating may a potential therapeutic direction for treatment Many Traditional Chinese Medicine (TCM) formulas some herbal extracts affect cell its metabolites. In this review, we mainly focus on role correlation with We also summarize current research progress regulation process TCM.

Язык: Английский

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Massa Medicata Fermentata treated spleen deficiency constipation by mediating intestinal microbiota and serum peptide

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Март 6, 2025

To investigate the correlation between treatment of spleen deficiency constipation and typical brain intestinal peptides. A total 18 male Kunming mice were randomly divided into three groups (n = 6): normal group (CC), model (CM), Massa Medicata Fermentata intervention (CG). CM CG used to establish a mouse model. After was finished, infused with 0.15 g/mL water infusion at dose 4 g/(kg·day), twice day, 0.4 mL. An equal amount distilled in CC for 7 days. The body weight fecal content monitored during modeling. Following intervention, 16S rRNA amplicon sequencing analyze changes microflora contents, serum substance P (SP), vasoactive peptide (VIP), calcitonin gene-related (CGRP) levels determined via ELISA. modeling had no significant effect on mice, mice's feces greatly reduced, dry hard. Constipation caused by can lead decrease SP an increase VIP CGRP. Fermentata, SP, VIP, CGRP all changed. Intestinal microbiota diversity constipation, dominant characteristic changed, indicating that unbalanced. increased; became Candidatus Arthromitus, Lactobacillus, unclassified Bacilli, Bacillus, Ligilactobacillus, Muribaculaceae, Bacteroides, Enterorhabdus; Arthromitus. Through analysis levels, Ligilactobacillus found be positively correlated negatively Akkermansia Streptococcus Arthromitus CGRP, affect secretion short-chain fatty acids intestine altering microecological environment intestine, then peptides alleviate constipation.

Язык: Английский

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Association of intestinal mucosal barrier function with intestinal microbiota in Spleen-Kidney Yang Deficiency IBS-D mice

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Апрель 29, 2025

To establish and evaluate an IBS-D mouse model with Spleen-Kidney Yang Deficiency, explore the microecological mechanisms of IBS-D, provide experimental evidence for clinical diagnosis treatment Deficiency. SPF-grade female Kunming mice were used to Deficiency through Folium senna-adenine administration combined restraint-clamping tail. (1) Clinical symptoms signs assessed using diagnostic criteria. (2) The small intestine structure was examined via Alcian blue staining, intestinal barrier markers like D-LA (D-lactate) DAO (diamine oxidase) measured by ELISA assess pathophysiological changes. (3) 16S rRNA gene sequencing performed analyze microbiota. (I) exhibited (II) alcian staining revealed elevated levels activity in group, indicating damage mucosal structure. (III) Analysis microbiota group differences dominant characteristic bacteria at various taxonomic compared those normal reflecting imbalance (IV) Lactobacillus Lentilactobacillus are associated modeled combination tail can be successfully This leads Streptococcus, Serratia, Helicobacter, Phocaeicola, Desulfomicrobium may serve as potential biological

Язык: Английский

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Huoxiang Zhengqi decoction ameliorates gastrointestinal disorders induced by cold and humid environmental stress via modulation of intestinal mucosal microbiota and amino acid metabolism

3 Biotech, Год журнала: 2025, Номер 15(6)

Опубликована: Май 3, 2025

Язык: Английский

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Arecoline Triggers Psychostimulant Responses by Modulating the Intestinal Microbiota to Influence Neurotransmitter Levels and Digestive Enzyme Activity

Pharmaceuticals, Год журнала: 2025, Номер 18(6), С. 794 - 794

Опубликована: Май 25, 2025

Background: Areca catechu L. is an evergreen tree belonging to the Arecaceae family. As important traditional Chinese medicine, it has wide applications in field of herbal medicine. Arecoline main active component responsible for its medicinal effects and plays a key role central nervous system (CNS) stimulant properties. Methods: This study investigated excitatory arecoline by analyzing behavioral changes mice, neurotransmitter levels, intestinal microbiota composition, enzymatic activities. We further explored bidirectional interactions between microbial ecosystem following exposure. Results: administration significantly increased activity time ratio mice (p < 0.05). It also elevated fecal lactase amylase activities 0.05), suggesting enhanced carbohydrate metabolism that may be one reasons mice. Serum analysis showed decreased 5-hydroxytryptamine (5-HT, p dopamine (DA) brain-derived neurotrophic factor (BDNF) levels 0.001), no significant change γ-aminobutyric acid (GABA). These findings suggest play modulating balance. At genus level, Escherichia was enriched positively correlated with DA, BDNF, GABA, while Clostridium abundance 5-HT. Conclusions: altered multiple composition mouse intestine, eliciting psychostimulant maintaining homeostasis. provides experimental foundation pharmacological exploitation arecoline.

Язык: Английский

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