Highly sensitive multiplexed colorimetric lateral flow immunoassay by plasmon-controlled metal–silica isoform nanocomposites: PINs

Nano Convergence, Год журнала: 2024, Номер 11(1)

Опубликована: Окт. 24, 2024

Abstract Lateral flow assay (LFA) systems use metal nanoparticles for rapid and convenient target detection are extensively studied the diagnostics of various diseases. Gold (AuNPs) often used as probes in LFAs, displaying a single red color. However, there is high demand colorimetric LFAs to detect multiple biomarkers, requiring multicolored NPs. Here, we present highly sensitive multiplexed lateral immunoassay by Plasmon-controlled metal–silica Isoform Nanocomposites (PINs). We utilized localized surface plasmon resonance effect create multi-colored PINs precisely adjusting distance between NPs on through controlled addition reduced gold silver precursors. Through simulations, also confirmed that significantly affects color signal intensity PINs. achieved exhibit stronger signals, offering new solution LFA with sensitivity 33 times limit (LOD) while maintaining consistent size deviations within 5%. expect our PINs-based will facilitate simultaneous biomarkers point-of-care testing. Graphical

Язык: Английский

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Bidirectionally Favorable Platform: A Dual-Targeting Probe-Encoded Maple Leaf-Type Fluorescent Lateral Flow Immunoassay for Multiple Biomarker Detection

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 17, 2025

In the traditional multiplexed lateral flow immunoassay (LFIA), different detection probes against targets are necessary. However, relative complexity and high cost of probe preparation, as well insufficient user-friendliness, limit application LFIA in disease diagnosis. Here, we reported a bidirectionally favorable (BDF-LFIA) platform to maximize convenience for both manufacturers users. Red-emitting time-resolved fluorescent nanoparticles were coated with antibodies recognize multiple simultaneously, which greatly simplified preparation by manufacturers. Ultrabright green-emitting gold nanoclusters pre-embedded on test line reference signal achieve target concentration-dependent maple leaf-type hue readout from green yellow red, was quite user-friendly. Taking cancer biomarkers alpha-fetoprotein carcinoembryonic antigen examples, this assay achieved visual 2 ng/mL. Compared conventional LFIA, BDF-LFIA could generate more discernible around threshold concentration targets. Moreover, successfully diagnosed 54 clinical samples. Overall, showed bidirectional benefits users provided new concept detection.

Язык: Английский

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A Robust, Solvent-Resistant Paper-Based Platform for Discriminative Detection of Organic Solvents

BioChip Journal, Год журнала: 2025, Номер unknown

Опубликована: Май 23, 2025

Язык: Английский

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Nanoparticle-integrated hydrogels as versatile colorimetric sensors

Coordination Chemistry Reviews, Год журнала: 2025, Номер 541, С. 216835 - 216835

Опубликована: Май 29, 2025

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Dynamically interactive nanoparticles in three-dimensional microbeads for enhanced sensitivity, stability, and filtration in colorimetric sensing

Advanced Composites and Hybrid Materials, Год журнала: 2024, Номер 7(6)

Опубликована: Ноя. 19, 2024

Язык: Английский

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Designing Optimal Probe Sequence for Breast Cancer Subtype Classification via Multiple MicroRNAs Recognition

BioChip Journal, Год журнала: 2024, Номер unknown

Опубликована: Сен. 26, 2024

Язык: Английский

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