Genomic insights into CKX genes: key players in cotton fibre development and abiotic stress responses

PeerJ, Год журнала: 2024, Номер 12, С. e17462 - e17462

Опубликована: Май 30, 2024

Cytokinin oxidase/dehydrogenase (CKX), responsible for irreversible cytokinin degradation, also controls plant growth and development response to abiotic stress. While the CKX gene has been studied in other plants extensively, its function cotton is still unknown. Therefore, a genome-wide study identify family four species was conducted using transcriptomics, quantitative real-time PCR (qRT-PCR) bioinformatics. As result, G. hirsutum barbadense (the tetraploid species), 87 96 genes respectively 62 each arboreum raimondii, were identified. Based on evolutionary studies, divided into five distinct subfamilies. It observed that have conserved sequence logos expansion due segmental duplication or whole genome (WGD). Collinearity multiple synteny studies showed an of families during evolution purifying selection pressure exerted. displayed exons/introns, uneven chromosomal distribution, protein motifs, cis-elements related stress their promoter regions. Cis-elements resistance, physiological metabolism hormonal regulation identified within regions genes. Expression analysis under different conditions (cold, heat, drought salt) revealed expression patterns tissues. Through virus-induced silencing (VIGS), GhCKX34A found improve cold resistance by modulating antioxidant-related activity. Since GhCKX29A highly expressed fibre development, we hypothesize increased fibres significant effects elongation. Consequently, these results contribute our understanding involvement GhCKXs both

Язык: Английский

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Molecular Mechanisms of Cold Stress Response in Cotton: Transcriptional Reprogramming and Genetic Strategies for Tolerance

Plant Science, Год журнала: 2025, Номер unknown, С. 112390 - 112390

Опубликована: Янв. 1, 2025

Язык: Английский

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Overexpression of SiICE2 gene reduces plant height and increases yield in cotton (Gossypium hirsutum)

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Июнь 2, 2025

Cotton (Gossypium hirsutum) is the predominant global fiber crop and has considerable significance because of its agronomic characteristics. Among these characteristics, plant height plays a vital role in determining harvest index yield potential. Previous studies have mainly focused on elucidating mechanisms governing ICE-CBF-COR cascade to enhance resilienceto low temperatures. Although ICE2 lines did not show cold tolerance, they exhibited dwarfing plants increased yield. This led observations reduced hypocotyl internode length, biomass, higher yield, shorter length through phenotypic assessment. From leaf transcriptome analysis, differential expression BR synthesis signaling pathway CBF1 gene was identified. qRT-PCR analysis revealed upregulation GhCBF1 downregulation GhTCH4 stems lines. Significantly, application BL spray an increase cotton height, with demonstrating highest elongation rate. In conclusion, reduction observed can be ascribed integration SiICE2 influencing cotton, which inhibits cell results plants. To provide reference for optimization agricultural production.

Язык: Английский

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Genome-wide identification of isopentenyl transferase genes in cotton and their roles in regulating vegetative branching after topping

Industrial Crops and Products, Год журнала: 2024, Номер 223, С. 119853 - 119853

Опубликована: Окт. 21, 2024

Язык: Английский

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Overexpression of Sikice2 Gene Reduces Plant Height and Increases Yield in Cotton (Gossypium Hirsutum L.)

Опубликована: Янв. 1, 2024

Язык: Английский

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Development of High-Yielding Upland Cotton Genotypes with Reduced Regrowth after Defoliation Using a Combination of Molecular and Conventional Approaches

Genes, Год журнала: 2023, Номер 14(11), С. 2081 - 2081

Опубликована: Ноя. 15, 2023

Cotton is an economically important crop. However, the yield gain in cotton has stagnated over years, probably due to its narrow genetic base. The introgression of beneficial variations through conventional and molecular approaches helped broaden base some extent. growth habit one crucial factors that determine crop maturation time, yield, management. This study used 44 diverse upland genotypes develop high-yielding germplasm with reduced regrowth after defoliation early maturity by altering from perennial somewhat annual. We selected eight top-scoring based on gene expression analysis five floral induction meristem identity genes (FT, SOC1, LFY, FUL, AP1) them make a total 587 crosses 30 different combinations these genotypes. High-performance progeny lines were phenotypic data plant height, flower boll numbers per plant, opening date, clustering, as surrogates annual habit, collected four years (2019 2022). Of lines, 8×5-B3, 8×5-B4, 9×5-C1, 8×9-E2, 8×9-E3, 39×5-H1 showed maturity, 20×37-K1, 20×37-K2, 20×37-D1 clustered flowering, regrowth, high quality fiber, lint yield. In 2022, 15 advanced (F8/F7) seven cross sent for increase Costa Rica winter nursery be testing release lines. addition breeding we developed resources breed improved converting expression-trait-associated SNP markers identified earlier into user-friendly allele-specific PCR-based assay tested parental F2 population.

Язык: Английский

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Plant cytogenetics blurring disciplinary boundaries to sustain global food security

The Nucleus, Год журнала: 2023, Номер 66(3), С. 239 - 243

Опубликована: Ноя. 18, 2023

Язык: Английский

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