Disruption of the endoplasmic reticulum-localized fatty acyl-ACP thioesterase IPF1 caused partial male sterility in rice

Plant Molecular Biology, Год журнала: 2025, Номер 115(2)

Опубликована: Март 8, 2025

Язык: Английский

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Onion pollenkitt: Function, DNase activity, fatty acid composition, and SEM imaging

PLoS ONE, Год журнала: 2025, Номер 20(4), С. e0321197 - e0321197

Опубликована: Апрель 7, 2025

Pollenkitt, a sticky substance produced by the tapetal cells of pollen grains, plays crucial role in functionality, yet it remains an understudied component plant biology. In this study, we investigated pollenkitt onion ( Allium cepa ) pollen, focusing on its effects germination, DNase activity, fatty acid composition, and ultrastructure. Our findings reveal that is essential for successful germination tube growth stigma, as removal significantly inhibited these processes. Additionally, demonstrated exhibits degrading plasmid DNA concentration-dependent manner, with EDTA effectively inhibiting degradation. Gas chromatography identified 20 acids pollenkitt, predominance unsaturated acids. Using scanning electron microscopy (SEM), showed structural differences between grains without observed process from surface water washing. These results offer valuable insights future research aimed at optimizing pollen-mediated gene-editing systems highlight importance considering such applications.

Язык: Английский

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Relationship Between Open Reading Frame 320, a Gene Causing Male Sterility in Tomatoes, and Cytoplasmic Male Sterility in Potatoes

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 18, 2025

Abstract Cytoplasmic male sterility (CMS) is a phenotype wherein plants cannot develop normal organs because of mitochondrial genes. Several cytoplasmic factors are suggested to be involved in CMS phenotypes potatoes ( Solanum tuberosum ); however, reports on the relevant genes remain scarce. Many potato cultivars carry that cause CMS, resulting pollen sterility, thereby limiting their use as parents breeding. Therefore, identifying causal crucial for In this study, we focused T/β-type cytoplasm, most prevalent genome type worldwide, explore potatoes. We identified novel gene, open reading frame 320 orf320 ), genotypes with cytoplasm by comparing genomes Functional analysis tomatoes showed overexpression transit peptide induced phenotypes, including abnormal anther development and abortion. Furthermore, an investigation 126 revealed gene specific absent investigated other types. These findings provide strong evidence candidate CMS-causing offering valuable insights future

Язык: Английский

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Characterization and transcriptome analysis reveal abnormal pollen germination in cytoplasmic male sterile tomato

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 22, 2025

Abstract Cytoplasmic male sterility (CMS) is a phenomenon resulting from mitochondrial-nuclear incompatibility that widely used in hybrid breeding. Although the mitochondrial gene orf137 has been identified as causal CMS tomatoes, its function remains unclear. In this study, we characterized male-sterile phenotypes and analyzed pollen transcriptome. Microscopic calcium imaging analyses revealed exhibited abnormal germination multiple apertures, accompanied by elevated concentrations vesicle accumulation, which are typically observed tube tips. RNA-Seq analysis 440 differentially expressed genes, including four pectin methylesterase inhibitor (PMEI ) genes were highly pollen. PME activity was significantly reduced pollen, suggesting association with germination. ATP reactive oxygen species (ROS) levels, key mediators of retrograde signaling (MRS), remained unchanged expression stress marker AOX1 not elevated. These findings suggest triggers an alternative MRS pathway independent or ROS, potentially leading to PMEI upregulation Our results reveal previously unrecognized mechanism CMS-induced tomatoes involving nuclear regulation through unconventional signaling.

Язык: Английский

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Ultrastructural study of pollen and tapetum development in Hydrocleys nymphoides, Alisma plantago-aquatica, and Sagittaria montevidensis (Alismataceae)

PROTOPLASMA, Год журнала: 2024, Номер unknown

Опубликована: Сен. 13, 2024

Язык: Английский

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