Peptidomics

Nature Reviews Methods Primers, Год журнала: 2023, Номер 3(1)

Опубликована: Март 30, 2023

Язык: Английский

---

Neuropeptidergic Systems in Pluteus Larvae of the Sea Urchin Strongylocentrotus purpuratus: Neurochemical Complexity in a “Simple” Nervous System

Frontiers in Endocrinology, Год журнала: 2018, Номер 9

Опубликована: Окт. 25, 2018

The nervous system of the free-living planktonic larvae sea urchins is relatively "simple", but sufficiently complex to enable sensing environment and control swimming feeding behaviours. At pluteus stage development, comprises a central ganglion serotonergic neurons located in apical organ sensory motor associated with ciliary band gut. Neuropeptides are key mediators neuronal signaling systems currently little known about neuropeptidergic urchin larvae. Analysis genome sequence Strongylocentrotus purpuratus has enabled identification thirty-eight genes encoding neuropeptide precursors (NP) this species. Here we characterise for first time expression nine these NP S. larvae, providing basis functional understanding neurochemical organization larval system. In order accomplish used single double situ hybridization, coupled immunohistochemistry, investigate gene comparison markers (e.g. neurotransmitter serotonin). Several sub-populations cells that express one or more were identified, which plate, at base arms, around mouth, mid- fore-gut. Furthermore, high levels cell proliferation observed neurogenic territories, consistent an increase number late stages. This study revealed far level than was previously known. Our map provides future work, aimed role diverse neuropeptides various aspects embryonic behaviour.

Язык: Английский

---

The neuropeptidomes of the sea cucumbers Stichopus cf. horrens and Holothuria scabra

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 27, 2025

The Philippines is a renowned marine biodiversity hotspot, home to several sea cucumber species with unusual biological traits. Among these, Stichopus cf. horrens notable for its ability undergo rapid body wall liquefaction when stressed, coupled remarkable regenerative abilities. In contrast, Holothuria scabra has one of the most robust walls in cucumbers and thrives many regimes tropics. Despite their intriguing traits, neurobiology chemical diversity these remain underexplored. Neuropeptides are important components an animal's neurobiological toolkit that underlie various physiological behavioral processes. Thus, discovery neuropeptides crucial step understanding molecular underpinnings unique traits cucumbers. Leveraging throughput sensitivity tandem mass spectrometry, we obtained unbiased view endogenous peptidomes radial nerve cord tissues non-model species, H. S. horrens. this work, sequenced 60 mature peptides from were derived 22 precursor proteins, 43 originating 25 proteins nervous tissues. A total seven previously unannotated uncharacterized neuropeptide precursors identified, thereby expanding known animal repertoire. Furthermore, discovered consistent structural features based on type post-translational modifications while pushing forward potentially novel proteolytic processing sites during peptide maturation enriched flanking amino acid residues. Collectively, our results provide preliminary data expand echinoderm through discovery, paving way innovative solutions address global demand echinoderms.

Язык: Английский

---

Identification and characterization of neuropeptides in sea urchin Strongylocentrotus intermedius

General and Comparative Endocrinology, Год журнала: 2025, Номер unknown, С. 114716 - 114716

Опубликована: Март 1, 2025

Язык: Английский

---

Discovery and functional characterization of neuropeptides in crinoid echinoderms

Frontiers in Neuroscience, Год журнала: 2022, Номер 16

Опубликована: Дек. 13, 2022

Neuropeptides are one of the largest and most diverse families signaling molecules in animals and, accordingly, they regulate many physiological processes behaviors. Genome transcriptome sequencing has enabled identification genes encoding neuropeptide precursor proteins species from a growing variety taxa, including bilaterian non-bilaterian animals. Of particular interest deuterostome invertebrates such as phylum Echinodermata, which occupies phylogenetic position that facilitated reconstruction evolution systems Bilateria. However, our knowledge echinoderms is largely based on bioinformatic experimental analysis eleutherozoans-Asterozoa (starfish brittle stars) Echinozoa (sea urchins sea cucumbers). Little known about crinoids (feather stars lilies), sister clade to Eleutherozoa. Therefore, we have analyzed transcriptome/genome sequence data three feather star species, Anneissia japonica, Antedon mediterranea, Florometra serratissima, produce first comprehensive precursors crinoids. These include representatives several predicted crinoid precursors. Using A. mediterranea an model, investigated expression selected neuropeptides larvae (doliolaria), post-metamorphic pentacrinoids adults, providing new insights into cellular architecture nervous systems. Thus, using mRNA situ hybridization F-type SALMFamide transcripts were revealed previously undescribed population peptidergic cells located dorso-laterally doliolaria. Furthermore, immunohistochemistry calcitonin-type was aboral nerve center, circumoral ring oral tube feet ectoneural entoneural compartments system adults. Moreover, functional vasopressin/oxytocin-type (crinotocin), expressed brachial arms this peptide causes dose-dependent change mechanical behavior arm preparations vitro-the reported biological action crinoid. In conclusion, findings provide perspectives foundations for further exploration expression/function eleutherozoan echinoderms.

Язык: Английский

---

Recent advances in mass spectrometry analysis of neuropeptides

Mass Spectrometry Reviews, Год журнала: 2021, Номер 42(2), С. 706 - 750

Опубликована: Сен. 24, 2021

Abstract Due to their involvement in numerous biochemical pathways, neuropeptides have been the focus of many recent research studies. Unfortunately, classic analytical methods, such as western blots and enzyme‐linked immunosorbent assays, are extremely limited terms global investigations, leading researchers search for more advanced techniques capable probing entire neuropeptidome an organism. With technological advances, mass spectrometry (MS) has provided methodology gain knowledge a on spatial, temporal, quantitative level. This review will cover key considerations analysis by MS, including sample preparation strategies, instrumental advances identification, structural characterization, imaging; insightful functional studies; newly developed absolute relative quantitation strategies. While discoveries made with is still its infancy. Many current challenges areas that need development also be highlighted this review.

Язык: Английский

---

Nervous System Development and Neuropeptides Characterization in Embryo and Larva: Insights from a Non-Chordate Deuterostome, the Sea Cucumber Apostichopus japonicus

Biology, Год журнала: 2022, Номер 11(10), С. 1538 - 1538

Опубликована: Окт. 20, 2022

Here, we described the complex nervous system at five early developmental stages (blastula, gastrula, auricularia, doliolaria and pentactula) of a holothurian species with highly economic value, Apostichopus japonicus. The results revealed that embryos larvae is mainly distributed in anterior apical region, ciliary bands or rings, feeding attachment organs, serotonergic immunoreactivity was not observed until embryo developed into late gastrula; these are evolutionarily conserved features echinoderm, hemichordate protostome larvae. Furthermore, based on available transcriptome data, reported neuropeptide precursors profile different embryonic larval stages. This analysis showed 40 present adult sea cucumbers were also identified larvae, only four (SWYG precursor 2, GYWKDLDNYVKAHKT precursor, Neuropeptide 14-like GLRFAmprecursor-like precursor) predicted adults absent Combining quantitative expression ten specific genes (NPs) by qRT-PCR, potential important roles neuropeptides development, A. japonicus In conclusion, this work provides novel perspectives diverse physiological functions contributes to understanding evolution neuropeptidergic systems echinoderm

Язык: Английский

---

Exploring neuropeptide signalling through proteomics and peptidomics

Expert Review of Proteomics, Год журнала: 2018, Номер 16(2), С. 131 - 137

Опубликована: Дек. 21, 2018

Introduction: Neuropeptides are neuro-endocrine signaling molecules capable of as neurotransmitters, neuromodulators or neurohormones. Studying how neuropeptide is integrated in endocrine pathways and neuropeptides regulate endogenous processes crucial to understanding multicellular organisms respond environmental internal cues.Areas covered: This review will cover proteomics peptidomics approaches used researching peptide systems breakthroughs that were achieved this field. Both differential mass spectrometry reverse genetic commonly study neuropeptidergic signaling. The field quickly developed the past decades expanded from gel-based include advanced liquid chromatography spectrometry. We explore reveal maturation identify downstream targets pathways. show differs standard it both processing signal pathway identification.Expert commentary: Thanks recent advancements isolation techniques increased sensitivity equipment, studies contributing increasingly elucidating functional implications Further technical progress should allow for full peptidomic profiling single neurons, eventually providing us with a complete comprehension

Язык: Английский

---

Effects of neurotransmitter receptor antagonists on sea urchin righting behavior and tube foot motility

Journal of Experimental Biology, Год журнала: 2022, Номер 225(6)

Опубликована: Фев. 28, 2022

Echinoderms, such as sea urchins, occupy an interesting position in animal phylogeny that they are genetically closer to vertebrates than the vast majority of all other invertebrates but have a nervous system lacks brain or brain-like structure. Despite this, very little is known about neurobiology adult urchin, and how utilized produce behavior. Here, we investigated effects on righting response antagonists ionotropic receptors for neurotransmitters acetylcholine, GABA glycine, metabotropic amines dopamine noradrenaline (norepinephrine). Antagonists slowed dose-dependent manner, with rank order potency strychnine>haloperidol>propranolol>bicuculline>hexamethonium, RT50 values (concentrations time by 50%) ranging from 4.3 µmol l-1 strychnine 7.8 mmol hexamethonium. The results also showed both glycine adrenergic needed actual tube foot movement, this may explain seen when these were inhibited. Conversely, inhibition had no effect motility, indicating play roles neural processing involved behavior, rather physical righting. Our identify first inhibiting glycinergic, dopaminergic neurotransmitter systems urchins distinguish between ability right themselves their move feet.

Язык: Английский

---

Protein- und Peptide-Hormone von Invertebraten

Springer eBooks, Год журнала: 2020, Номер unknown, С. 205 - 282

Опубликована: Ноя. 17, 2020

---