The
increasing
number
of
deaths
caused
by
mosquito-borne
diseases
highlights
the
need
to
produce
efficient
and
environmentally
sustainable
alternatives.
aim
present
investigation
we
have
utilized
leaf
from
Laureliopsis
philippiana
(LP)
synthesize
silver
(Ag)
palladium
(Pd)
nanoparticles
(NPs)
studied
toxicity
on
deadly
disease
vectors
larvae
Aedes
aegypti
Anopheles
stephensi.
Biosynthesized
Ag
Pd
NPs
were
investigated
UV-Vis
spectroscopy,
Fourier
transform
infrared
(FT-IR),
X-Ray
Diffraction
(XRD),
Transmission
electron
microscopy
(TEM),
Zeta
potential
analysis.
GC-MS
analysis
L.
assisted
in
identifying
secondary
metabolites
extract's
total
flavonoid
phenolic
compounds,
which
are
reactants
bio-reduction
process
during
production
nanoparticles.
in-silico
was
conducted
mosquito
sterol
carrier
protein-2
(1PZ4)
odorant
binding
protein
(2L2C).
XRD
results
revealed
crystalline
structure
NPs.
At
same
time,
TEM
representation,
biosynthesized
spherical,
clustered,
exhibited
a
noticeable
UV
peak
at
417
370
nm
(average
size
AgNPs:
13.25±
2.53
PdNPs:
4.07±
1.64
nm).
biogenic
concentration-dependent
larvicidal
effect
with
LC50
values
29.596,
17.804,
23.915,
14.878
µg/mL
Ae.
23.946,
13.938,
19.457,
12.876
against
An.
stephensi,
correspondingly.
In-silico
studies
proved
that
Laureliopsine
A
(-7.6
-8.4
kcal/mol)
isoterpinolene
(-7.0
-6.7
showed
significant
docking
scores
comparison
permethrin
(-5.3
-8.0
kcal/mol),
proteins
(1PZ4
2L2C).
Besides,
LP-Ag
non-toxicity
carried
out
Zebrafish
embryos
72h
post-fertilization.
Overall,
findings
clearly
demonstrated
green
synthesized
develop
secure
safe
larvicides.
Uncontrolled
applications
of
weedicide
and
fertilizer
can
harm
the
soil
ecology,
most
significantly,
earthworms
are
hazardous
engineers.
Thus,
we
aimed
at
toxicity
histopathological
alterations
in
earthworm
Eudrilus
euginae
following
exposure
to
glyphosate
(weedicide),
urea
(fertilizer),
environmentally
friendly
dual
herb-mixed
silver
nanoparticles
(DHM-AgNPs).
The
DHM-AgNPs
were
synthesized
using
a
blend
Alfinia
officinarum
Curcuma
longa
aqueous
leaf
extracts
with
1
mM
nitrate.
color
change
from
yellow
brown
after
an
hour
incubation
was
significant
indicator
successful
DHM-AgNP
synthesis.
Characterization
UV-vis
spectra
indicated
surface
plasmon
resonance
(SPR)
peak
430
nm.
identified
FT-IR
spectroscopy,
XRD,
SEM,
TEM
investigation.
Furthermore,
different
concentrations
administered
Artemia
nauplii
E.
assess
their
toxicity.
mortality
rate
for
exposed
higher
concentration
(10
g/kg
soil)
100%.
In
contrast,
83%
noted
0.5
soil.
maximum
(90
±
0.64%)
observed
10
mL/kg/L
glyphosate.
low
A.
gradient
compared
urea.
Additionally,
evaluated
anti-Vibrio
activity
against
pathogenic
Vibrio-related
bacteria,
displaying
inhibition
(80%)
100
µg/L.
This
study
suggests
potential
use
field
crops
eco-friendly
pest
control
without
causing
environmental
pollution.
Further
research
is
warranted
determine
efficacy,
safety,
cost-effectiveness
agricultural
practices.
The
increasing
number
of
deaths
caused
by
mosquito-borne
diseases
highlights
the
need
to
produce
efficient
and
environmentally
sustainable
alternatives.
aim
present
investigation
we
have
utilized
leaf
from
Laureliopsis
philippiana
(LP)
synthesize
silver
(Ag)
palladium
(Pd)
nanoparticles
(NPs)
studied
toxicity
on
deadly
disease
vectors
larvae
Aedes
aegypti
Anopheles
stephensi.
Biosynthesized
Ag
Pd
NPs
were
investigated
UV-Vis
spectroscopy,
Fourier
transform
infrared
(FT-IR),
X-Ray
Diffraction
(XRD),
Transmission
electron
microscopy
(TEM),
Zeta
potential
analysis.
GC-MS
analysis
L.
assisted
in
identifying
secondary
metabolites
extract's
total
flavonoid
phenolic
compounds,
which
are
reactants
bio-reduction
process
during
production
nanoparticles.
in-silico
was
conducted
mosquito
sterol
carrier
protein-2
(1PZ4)
odorant
binding
protein
(2L2C).
XRD
results
revealed
crystalline
structure
NPs.
At
same
time,
TEM
representation,
biosynthesized
spherical,
clustered,
exhibited
a
noticeable
UV
peak
at
417
370
nm
(average
size
AgNPs:
13.25±
2.53
PdNPs:
4.07±
1.64
nm).
biogenic
concentration-dependent
larvicidal
effect
with
LC50
values
29.596,
17.804,
23.915,
14.878
µg/mL
Ae.
23.946,
13.938,
19.457,
12.876
against
An.
stephensi,
correspondingly.
In-silico
studies
proved
that
Laureliopsine
A
(-7.6
-8.4
kcal/mol)
isoterpinolene
(-7.0
-6.7
showed
significant
docking
scores
comparison
permethrin
(-5.3
-8.0
kcal/mol),
proteins
(1PZ4
2L2C).
Besides,
LP-Ag
non-toxicity
carried
out
Zebrafish
embryos
72h
post-fertilization.
Overall,
findings
clearly
demonstrated
green
synthesized
develop
secure
safe
larvicides.
