Evaluation of the Phytochemical Screening of Methanolic Seed Extracts of Tribulus terrestris: An In Vitro Application of Anti-cancer, Anti-oxidant, and Anti-microbial Activities DOI Open Access

Mohammed Yaseen Malik,

Arockia Alex,

Azhagu Madhavan Sivalingam

и другие.

Cureus, Год журнала: 2024, Номер unknown

Опубликована: Авг. 12, 2024

Background: Tribulus terrestris, a plant known for its pharmacological properties, was investigated in this study potential anticancer effects against oral cancer cells. The aimed to explore the phytochemical composition of T. terrestris seed extract and evaluate cytotoxic, pro-apoptotic, antioxidant, anti-inflammatory, antimicrobial activities. Materials methods: Methanolic extracts were obtained subjected analysis identify bioactive compounds. cytotoxic effect on cells evaluated using MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay, while pro-apoptotic assessed through dual fluorescent staining. Antioxidant activity measured hydrogen peroxide erythrocyte aggregation assays, anti-inflammatory inhibition albumin denaturation. Results: Phytochemical revealed presence alkaloids, tannins, saponins, flavonoids, phenols extract. demonstrated concentration-dependent cytotoxicity cells, with 100 μg/mL showing significant growth inhibition. Pro-apoptotic observed, characteristic morphological changes treated by extract, methanol fraction flower (MFF) exhibiting highest capacity, followed total trichome (TTF), positive correlation between phenolic content free radical scavenging effectiveness noted. Antimicrobial various pathogens, including bacteria fungi, also higher concentrations increased efficacy. Conclusion: concludes that methanolic possess anticancer, These findings highlight as candidate further research clinical applications, either alone or combination other agents, treatment associated conditions.

Язык: Английский

Recent advances in nanoformulation-based delivery for cancer immunotherapy DOI

Seyedeh Saimeh Bokhari,

T. Ali, Muhammad Naeem

и другие.

Nanomedicine, Год журнала: 2024, Номер 19(14), С. 1253 - 1269

Опубликована: Май 8, 2024

Cancer is one of the leading causes mortality worldwide, and its treatment faces several challenges. Phytoconstituents derived from recently discovered medicinal plants through nanotechnology potentially target cancer cells via PI3K/Akt/mTOR pathways exert their effects selectively generation reactive oxygen species β-catenin inhibition, DNA damage, increasing caspase 3/9 p53 expression. These nanocarriers act specifically against different cell lines such as HT-29, MOLT-4 human leukemia MCF-7 SKOV-3, Caov-3, SW-626, HepG2, A-549, HeLa, MCF-7. This review comprehensively elaborates on cellular molecular mechanisms, therapeutic prospects various plant-mediated nanoformulations to attain a revolutionary shift in immunotherapy.

Язык: Английский

Процитировано

1
Biosynthesis of ZnO nanocomposites from Mentha spicata applications of antioxidant, antimicrobial and genotoxicity advances in MCF-7 cell line DOI Creative Commons
Azhagu Madhavan Sivalingam

Sensing and Bio-Sensing Research, Год журнала: 2024, Номер 46, С. 100707 - 100707

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

1
Evaluation of the Phytochemical Screening of Methanolic Seed Extracts of Tribulus terrestris: An In Vitro Application of Anti-cancer, Anti-oxidant, and Anti-microbial Activities DOI Open Access

Mohammed Yaseen Malik,

Arockia Alex,

Azhagu Madhavan Sivalingam

и другие.

Cureus, Год журнала: 2024, Номер unknown

Опубликована: Авг. 12, 2024

Background: Tribulus terrestris, a plant known for its pharmacological properties, was investigated in this study potential anticancer effects against oral cancer cells. The aimed to explore the phytochemical composition of T. terrestris seed extract and evaluate cytotoxic, pro-apoptotic, antioxidant, anti-inflammatory, antimicrobial activities. Materials methods: Methanolic extracts were obtained subjected analysis identify bioactive compounds. cytotoxic effect on cells evaluated using MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay, while pro-apoptotic assessed through dual fluorescent staining. Antioxidant activity measured hydrogen peroxide erythrocyte aggregation assays, anti-inflammatory inhibition albumin denaturation. Results: Phytochemical revealed presence alkaloids, tannins, saponins, flavonoids, phenols extract. demonstrated concentration-dependent cytotoxicity cells, with 100 μg/mL showing significant growth inhibition. Pro-apoptotic observed, characteristic morphological changes treated by extract, methanol fraction flower (MFF) exhibiting highest capacity, followed total trichome (TTF), positive correlation between phenolic content free radical scavenging effectiveness noted. Antimicrobial various pathogens, including bacteria fungi, also higher concentrations increased efficacy. Conclusion: concludes that methanolic possess anticancer, These findings highlight as candidate further research clinical applications, either alone or combination other agents, treatment associated conditions.

Язык: Английский

Процитировано

0