Trichoderma afroharzianum TRI07 metabolites inhibit Alternaria alternata growth and induce tomato defense-related enzymes

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Янв. 22, 2024

Abstract Identifying a viable substitute for the limited array of current antifungal agents stands as crucial objective in modern agriculture. Consequently, extensive worldwide research has been undertaken to unveil eco-friendly and effective capable controlling pathogens resistant presently employed fungicides. This study explores efficacy Trichoderma isolates combating tomato leaf spot disease, primarily caused by Alternaria alternata . The identified pathogen, A. Alt3, was isolated confirmed through ITS region (OQ888806). Six were assessed their ability inhibit Alt3 hyphal growth using dual culture, ethyl acetate extract, volatile organic compounds (VOCs) techniques. most promising biocontrol isolate T. afroharzianum TRI07 based on three markers: (OQ820171), translation elongation factor alpha 1 gene (OR125580), RNA polymerase II subunit (OR125581). extract subjected GC–MS analysis, revealing spathulenol, triacetin, aspartame main compounds, with percentages 28.90, 14.03, 12.97%, respectively. Analysis TRI07-VOCs solid-phase microextraction technique indicated that abundant included ethanol, hydroperoxide, 1-methylhexyl, 1-octen-3-one. When interacted 34 identified, major components including 1-octen-3-one, hexanedioic acid, bis(2-ethylhexyl) ester. In greenhouse experiment, treatment 48 h before inoculation (A3 treatment) resulted reduction disease severity (16.66%) incidence (44.44%). Furthermore, A3 led improved performance parameters increased chlorophyll content. After 21 days post-inoculation, associated production antioxidant enzymes (CAT, POD, SOD, PPO), while infected plants exhibited elevated levels oxidative stress markers MDA H 2 O HPLC analysis extracts from revealed higher phenolic acids such gallic, chlorogenic, caffeic, syringic, coumaric acids, well flavonoid catechin, rutin, vanillin. novelty lies bridging gap between strain-specific attributes practical application, enhancing understanding TRI07’s potential integrated pest management. concludes presents natural biological activity, effectively promoting plant growth. findings have implications agriculture, suggesting sustainable strategy can enhance crop resilience contribute management practices.

Язык: Английский

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Essential oils from fennel plants as valuable chemical products: gas chromatography–mass spectrometry, FTIR, quantum mechanical investigation, and antifungal activity

Biomass Conversion and Biorefinery, Год журнала: 2024, Номер unknown

Опубликована: Май 13, 2024

Abstract In the present study, biomass produced by fennel plants ( Foeniculum vulgare Mill.) was converted to yield bioactive chemicals, and hydrodistillation method used extract essential oils (EOs) from both leaves umbels. The antifungal activity of EOs tested using bioassay against development Fusarium oxysporum MW854649 Alternaria solani MT279570. Molecular spectroscopic detection techniques were evaluate EO products gas chromatography–mass spectrometer (GC–MS) Fourier transform infrared spectroscopy (FTIR). GC–MS equipped with single quadruple analyzers have been measure electron ionization (EI) mass spectra primary constituents at 70 eV. main chemical compounds in anethole, estragole, D-limonene, trans-β-ocimene, fenchone, percentages 37.94, 35.56, 17.46, 1.53, 1.49%, respectively. abundant umbels γ-terpinene, 51.18, 25.08, 12.22, 6.57, 2.86%, EI spectral fragmentation major fenchone has investigated. Umbels leaf 5000 mg/L displayed strongest suppression fungal growth A. , values 87.78% 79.63%, respectively, compared positive control (94.44%). showed highest inhibition F. oxysprium as (94.44%), 77.77% 72.96%, All important ions—including a few distinctive fragment ions—have comprehensive pathways defined. Based on EI, routes for identified. existence alkenes, aliphatic alcohols, ethers, carboxylic acids, ester compounds, alkanes, hydrogen-bonded phenols demonstrated FTIR analysis EOs. On other hand, reactive behavior studied molecules investigated two quantum mechanics method: modified neglect diatomic overlap (MNDO), semi-empirical method, density functional theory (DFT)/B3LYP hybrid 6-311G (d, p) basis set ground state phase. optimum geometries obtained through execution computations electrostatic potential. analytical calculated results then understand further medical applications.

Язык: Английский

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Fabrication of biodegradable and antibacterial films of chitosan/polyvinylpyrrolidone containing Eucalyptus citriodora extracts

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 266, С. 131001 - 131001

Опубликована: Март 26, 2024

Язык: Английский

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HPLC and GC-MS analyses of phytochemicals from Ficus carica leaf extract and essential oil along with their antimicrobial properties

Journal of Agriculture and Food Research, Год журнала: 2025, Номер unknown, С. 101687 - 101687

Опубликована: Фев. 1, 2025

Язык: Английский

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Green Synthesis of Silver and Ferric Oxide Nanoparticles Using Syzygium cumini leaf Extract and Their Antifungal Activity when Applied to Oak Wood and Paper Pulp from Imperata cylindrica Grass Biomass

Waste and Biomass Valorization, Год журнала: 2024, Номер unknown

Опубликована: Июнь 5, 2024

Язык: Английский

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Ethnobotany, total phenolic and flavonoid content in the species Corymbia citriodora (Hook.) K.D. Hill & L.A.S. Johnson

Journal of Applied Pharmaceutical Science, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

After the emergence of COVID-19, respiratory sequelae persisted, and one alternative worth reconsideration is Corymbia citriodora (Hook.) K.D. Hill & L.A.S. Johnson. The objective to determine ethnobotany total phenolic flavonoid content C. citriodora. ethnobotanical information was gathered using a semi-structured questionnaire from herbalists in markets city Trujillo, La Libertad, Peru. collected Agallpampa, Otuzco, identified Herbarium Truxillense. Three extracts were prepared evaluated (infuse, decoct, hydroethanolic). phenols quantified Folin Ciocalteau method, flavonoids aluminum chloride method. reported higher medicinal use bronchitis (39.1%), preparation frequent infusion, made leaves, often combination with Piper aduncum (35.7%). Total as 118.65 mg GAE 33.95 QCE per gram dried drug, hydroethanolic extract standing out. high presence bioactives directly related anti-inflammatory activity, suggesting that remains viable option demonstrates efficacy traditional context for improving conditions.

Язык: Английский

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Chemical composition and antibacterial activity of chaulmoogra oil from Hydnocarpus wightiana: A detailed analysis of volatile and fatty acid fractions

Phytochemistry Letters, Год журнала: 2024, Номер 65, С. 102 - 112

Опубликована: Дек. 31, 2024

Язык: Английский

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