ACS Applied Bio Materials, Год журнала: 2025, Номер unknown

Опубликована: Май 20, 2025

Lignocellulose recalcitrance remains a significant economic challenge in modern biomass conversion processes. Microbial strategies offer considerable promise for ecofriendly bioenergy generation. This study presents an advanced integrated approach that combines bacterial treatment with bioelectrochemical system (BES) to enhance the efficiency of lignocellulosic biomass. Unlike or sequential approaches, comparative evaluation two distinct pretreatment strategies, alkaline delignification and biological treatment, was conducted independently assess their individual effectiveness sugar cane bagasse (SCB) degradation performance microbial fuel cell (MFC). Biological B. subtilis alone yielded superior outcomes terms saccharification efficiency, growth, bioelectricity generation, as evidenced by higher open-circuit potentials MFC half-cell studies comparison alkali delignified SCB. Notably, increased cellulose content 72% reduced hemicellulose lignin approximately 0.84-fold, indicating effective enzymatic action. Metabolomic profiling identified 2846 metabolites significantly diverged between experimental groups. lignin-derived compounds such ferulic acid, syringic p-coumaric acid were detected at elevated levels, confirming enhanced ligninase activity pretreated Additionally, presence organic acids (e.g., acetic acid), amino acids, derivatives, resulting from breakdown cellulose, hemicellulose, lignin, provided essential substrates exoelectrogenic organisms BESs. integration led maximum power density 353 ± 5 mW/m2 current 200 3 mA/m2, demonstrating enhancement MFC. Furthermore, biotransformation SCB facilitated channeling into value-added products, increasing overall valorization. Thus, rational utilization underscores its potential scalable biorefinery applications broader implications sustainable production.

Язык: Английский