AggressiveKRASmutations direct TGF-β response towards partial EMT in patient-derived colorectal cancer tumoroids DOI Open Access
Theresia Mair,

Philip König,

Milena Mijović

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 29, 2024

Abstract Transforming growth factor beta (TGF-β) exhibits complex and context-dependent cellular responses. While it mostly induces tumor-suppressive effects in early stages of tumorigenesis, its tumor promoting properties are evident advanced disease. This TGF-β duality is still not fully understood, whether supports invasion metastasis by influencing cancer cells directly, or rather through the stromal compartment remains a matter debate. Here, we utilized library colorectal (CRC) patient-derived tumoroids (PDTs), representing spectrum stages, to study cell-specific responses TGF-β. Using medium conditions allowing for differentiation PDTs, observed induced early-stage tumoroids. PDTs with pathway mutations derived from metastatic tumors were insensitive treatment. Notably, one tumoroid line harboring an atypical KRAS Q22K mutation underwent partial epithelial-to-mesenchymal transition (EMT), associated morphological changes increased invasiveness. On molecular level, this was accompanied elevated expression mesenchymal genes, as well deregulation pathways matrix remodeling cell adhesion. Our results suggest that intrinsic critical determining -promoting effects.

Язык: Английский

Application of bioelectrical impedance detection techniques: cells and tissues DOI
Jianming Wen,

Pengjie Wu,

Jianping Li

и другие.

Biosensors and Bioelectronics, Год журнала: 2025, Номер 273, С. 117159 - 117159

Опубликована: Янв. 11, 2025

Язык: Английский

Процитировано

0
The atypical KRASQ22K mutation directs TGF‐β response towards partial epithelial‐to‐mesenchymal transition in patient‐derived colorectal cancer tumoroids DOI Creative Commons
Theresia Mair,

Philip König,

Milena Mijović

и другие.

Molecular Oncology, Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

Transforming growth factor beta (TGF‐β) exhibits complex and context‐dependent cellular responses. While it mostly induces tumor‐suppressive effects in early stages of tumorigenesis, tumor‐promoting properties are evident advanced disease. This TGF‐β duality is still not fully understood, whether supports invasion metastasis by influencing cancer cells directly, or rather through the stromal tumor compartment, remains a matter debate. Here, we utilized library colorectal (CRC) patient‐derived tumoroids (PDTs), representing spectrum stages, to study cell‐specific responses TGF‐β. Using conditions allowing for differentiation PDTs, observed TGF‐β‐induced early‐stage tumoroids, whereas more were less sensitive treatment. Notably, one tumoroid line harboring an atypical KRAS Q22K mutation underwent partial epithelial‐to‐mesenchymal transition (EMT), which was associated with morphological changes increased invasiveness. On molecular level, this accompanied elevated expression mesenchymal genes, as well deregulation pathways matrix remodeling cell adhesion. Our results suggest that cell‐intrinsic critical determining its effects.

Язык: Английский

Процитировано

0
Cell culture techniques for cancer research DOI
Preeti Jain, N. V. Joshi, Sadhna Aggarwal

и другие.

Methods in cell biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Процитировано

0
AggressiveKRASmutations direct TGF-β response towards partial EMT in patient-derived colorectal cancer tumoroids DOI Open Access
Theresia Mair,

Philip König,

Milena Mijović

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 29, 2024

Abstract Transforming growth factor beta (TGF-β) exhibits complex and context-dependent cellular responses. While it mostly induces tumor-suppressive effects in early stages of tumorigenesis, its tumor promoting properties are evident advanced disease. This TGF-β duality is still not fully understood, whether supports invasion metastasis by influencing cancer cells directly, or rather through the stromal compartment remains a matter debate. Here, we utilized library colorectal (CRC) patient-derived tumoroids (PDTs), representing spectrum stages, to study cell-specific responses TGF-β. Using medium conditions allowing for differentiation PDTs, observed induced early-stage tumoroids. PDTs with pathway mutations derived from metastatic tumors were insensitive treatment. Notably, one tumoroid line harboring an atypical KRAS Q22K mutation underwent partial epithelial-to-mesenchymal transition (EMT), associated morphological changes increased invasiveness. On molecular level, this was accompanied elevated expression mesenchymal genes, as well deregulation pathways matrix remodeling cell adhesion. Our results suggest that intrinsic critical determining -promoting effects.

Язык: Английский

Процитировано

0