Transcriptional induction by ecdysone in Drosophila salivary glands involves an increase in chromatin accessibility and acetylation DOI Creative Commons

А. А. Евдокимова,

Т. Д. Колесникова, M. Yu. Mazina

и другие.

Nucleic Acids Research, Год журнала: 2025, Номер 53(7)

Опубликована: Апрель 10, 2025

Abstract Transcriptional activation by 20-hydroxyecdysone (20E) in Drosophila provides an excellent model for studying tissue-specific responses to steroids. An increase the 20E concentration regulates degradation of larval and proliferation adult tissues during metamorphosis. To study 20E-dependent transcription, we used natural system controlling concentration—the E23 membrane transporter—which exports from cell. We artificially expressed suppress first wave 20E-inducible transcription at expression revealed a plethora genes salivary glands, while mildly affecting brain. described mechanisms transcriptional glands. depletion decreased binding Pol II TFIID subunit, TBP, promoters primary targets, demonstrating role initiation. At target loci, resulted malfunctioning sites co-bound with EcR CBP/Nejire enriched H3K27Ac mark inherent active enhancers. these sites, was found control chromatin accessibility acetylation. suggest that activity ‘active’ ecdysone-sensitive elements responsible status targets glands wandering larvae.

Язык: Английский

Transcriptional induction by ecdysone in Drosophila salivary glands involves an increase in chromatin accessibility and acetylation DOI Creative Commons

А. А. Евдокимова,

Т. Д. Колесникова, M. Yu. Mazina

и другие.

Nucleic Acids Research, Год журнала: 2025, Номер 53(7)

Опубликована: Апрель 10, 2025

Abstract Transcriptional activation by 20-hydroxyecdysone (20E) in Drosophila provides an excellent model for studying tissue-specific responses to steroids. An increase the 20E concentration regulates degradation of larval and proliferation adult tissues during metamorphosis. To study 20E-dependent transcription, we used natural system controlling concentration—the E23 membrane transporter—which exports from cell. We artificially expressed suppress first wave 20E-inducible transcription at expression revealed a plethora genes salivary glands, while mildly affecting brain. described mechanisms transcriptional glands. depletion decreased binding Pol II TFIID subunit, TBP, promoters primary targets, demonstrating role initiation. At target loci, resulted malfunctioning sites co-bound with EcR CBP/Nejire enriched H3K27Ac mark inherent active enhancers. these sites, was found control chromatin accessibility acetylation. suggest that activity ‘active’ ecdysone-sensitive elements responsible status targets glands wandering larvae.

Язык: Английский

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