Molecular Mechanisms of Neutrophil Extracellular Traps in Promoting Gastric Cancer Epithelial–Mesenchymal Transition Through SERPINE‐1 Expression

Journal of Biochemical and Molecular Toxicology, Год журнала: 2025, Номер 39(3)

Опубликована: Март 1, 2025

Gastric cancer remains a significant global health concern, with its progression and metastasis often associated epithelial-mesenchymal transition (EMT). This study investigated the role of neutrophil extracellular traps (NETs) in promoting gastric EMT by regulating SERPINE-1 expression, which encodes plasminogen activator inhibitor-1 (PAI-1). Western blot immunohistochemistry were used to detect protein expression. Cell Counting Kit-8 was tested for cell proliferation ability using clones. The gene knocked down lentivirus. Immunofluorescence co-expression proteins, Transwell assay wound-healing investigate migration cells. Experimental conclusions verified vivo nude mouse model. We first demonstrated overexpression PAI-1 tissues lines. Subsequently, we found that NETs significantly enhanced expression EMT-related markers. These changes accompanied increases invasion, migration, tumour sphere formation. To further elucidate mechanism, employed lentivirus-mediated knockdown reverse NET-induced phenotype effectively. Mechanistically, activated transforming growth factor (TGF)-β signalling pathway via as evidenced increased TGF-β1, TGF-βR1, TGF-βR2, phosphorylated Smad2/3 Smad4. Finally, experiments model liver confirmed NET-treated HGC-27 cells exhibited metastatic potential abrogated potential. Our findings reveal novel mechanism promote PAI-1-TGF-β axis. can be target treatment cancer, is closely related prognosis patients cancer. Therapeutic strategies targeting or may help prevent improve clinical outcomes patients.

Язык: Английский

Proteogenomic verifies targets underlying erythromycin alleviate neutrophil extracellular traps-induced inflammation

Respiratory Research, Год журнала: 2025, Номер 26(1)

Опубликована: Апрель 19, 2025

Язык: Английский

Identification and validation of a lenvatinib resistance-related prognostic signature in HCC, in which PFKFB4 contributes to tumor progression and lenvatinib resistance

BMC Gastroenterology, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 23, 2025

Язык: Английский