Microbiology Spectrum, Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

ABSTRACT Tylosin is a commonly used macrolide antibiotic, which utilized in livestock; its release through animal excrement can have detrimental environmental effects. Biodegradation of tylosin (TYL) an effective bioremediation method. In this study, we identified novel and efficient TYL-degrading bacterial strain, Kurthia gibsonii TYL-A1, capable degrading 75 mg/L TYL within 5 days at 30°C, pH 7, with 3% inoculum yeast extract as the nitrogen source. The bacterium degraded 99% days. Both intracellular extracellular enzymes collaborated to degrade TYL. Metabolites were analyzed by liquid chromatography-mass spectrometry (LC-MS), revealing that strain TYL-A1 could remove mycophenolic sugar, cleave ester bond, further into smaller molecules. toxicity degradation products was lower than parent compound natural products. Whole-genome sequencing results indicated genes encoding glycoside hydrolases glycosyltransferases, along metabolism-related genes, involved degradation. This study elucidated mechanism highlighted potential from environment. IMPORTANCE contamination has become hot issue, microbial removal systems been widely considered economical environmentally friendly alternative. Our proposed new pathway biological metabolic LC-MS metabolite analysis. provided genetic process explained effect on application actual wastewater highlights practical relevance pollution importance germplasm resources TYL-contaminated ecosystems. All all, our provides theoretical basis for reducing antibiotics environment promoting sustainable development ecological

Язык: Английский