Tissue Engineering and Regenerative Medicine, Год журнала: 2023, Номер 20(3), С. 325 - 327
Опубликована: Апрель 20, 2023
Язык: Английский
Molecular Nutrition & Food Research, Год журнала: 2024, Номер unknown
Опубликована: Окт. 2, 2024
Scope This study evaluates the potential of bioconverted garlic ferments (BGFs) to stimulate intestinal immune system and modulate cecal microbiota composition. Methods results In vitro, BGF significantly enhances Peyer's patch (PP)‐mediated bone marrow cell proliferation increases production interferon‐gamma (IFN‐γ), granulocyte macrophage‐colony stimulating factor (GM‐CSF), interleukin (IL)‐6, immunoglobulin A (IgA) but not IL‐4, IL‐5, E (IgE). Oral administration C3H/HeN mice for 4 weeks GM‐CSF (42.1–45.8 pg mL −1 ) IFN‐γ (6.5–12.1 levels in PP cells. also elevates tumor necrosis factor‐alpha (TNF‐α, 165.0–236.3 mg ), (2.4–3.0 ng (1.5–3.2 small fluid, TNF‐α (2.2–3.1 (10.3–0.21.5 mouse serum. Cecal microbial analysis reveals that Bacteroidota Verrucomicrobiota decreases Actinobacteria Bacillota at phylum level mice. At genus level, abundance Fusimonas (250 kg BW day Bacteroides (125 250 Akkermansia Bifidobacterium (62.5 Limosilactobacillus ). Conclusion provides first evidence BGF's ability gut microbiota, supporting its as a novel functional material enhance immunity.
Язык: Английский
Процитировано
1
Microbiological Research, Год журнала: 2024, Номер 292, С. 128013 - 128013
Опубликована: Дек. 12, 2024
Язык: Английский
Процитировано
1
ImmunoHorizons, Год журнала: 2023, Номер 7(11), С. 788 - 805
Опубликована: Ноя. 1, 2023
The oral route is effective and convenient for vaccine administration to stimulate a protective immune response. GALT plays crucial role in mucosal responses, with Peyer's patches (PPs) serving as the primary site of induction. A comprehensive understanding structures functions these enhancing vaccination strategies comprehending disease mechanisms; nonetheless, our current knowledge dogs remains incomplete. We performed immunofluorescence flow cytometry studies on canine PPs identify cell populations structures. also single-cell RNA sequencing (scRNA-seq) investigate subpopulations present at steady state dogs. generated validated an Ab specifically targeting M cells, which will be valuable tool elucidating Ag trafficking into Our findings pave way future responses enteropathies. Moreover, they add growing body immunology, further expanding complex system
Язык: Английский
Процитировано
2
Scientific Messenger of LNU of Veterinary Medicine and Biotechnology, Год журнала: 2023, Номер 25(112), С. 90 - 97
Опубликована: Дек. 3, 2023
The morphogenesis of immune formations duck intestines was studied. Material for histological studies taken from Blagovarsky cross broiler ducks aged one to 420 days. obtained sections were stained with Karatsi's hematoxylin and eosin – establish the features microscopic structure intestinal formations, Weigert's picrofuchsin according Van Gieson aniline blue in combination acid fuchsin Orange G Mallory identify collagen fibers, resorcin-fuchsin Weigert differentiation elastic fibers. To determine reticular impregnated 1–2 % argentum nitrate solution modified Kelemen method. Electron microscopy carried out study ultrastructure 180-day-old ducks. have established that wall places where are localized is formed by tunica mucosa, muscularis serosa, area which all changes unevenly asynchronously birds’ age. mucosa occupies largest area, asynchronously, reaching maximum values (from 71.66 ± 0.25 81.28 0.19 %) during first 25 days ducks' life. a smaller than mucosa. It decreases bird’s life, older birds it increases reaches (35.68 0.49–46.67 1.39 330- 420-day-old birds. serosa smallest area. In day-old birds, this indicator highest 2.47 0.07 6.20 0.02 age 1.24–6.52 times. Lymphoid tissue, determines functions located their muscularis. detected caecal diverticula one-day age, other structures 10–20 total lymphoid tissue day (27.03 0.88–31.72 0.04 150 Peyer's patches duodenum jejunum cecal (59.86 %, 59.58 1.28, 65.12 0.50 %), up 210 Peyer’s ileum (71.64 1.03 330 cecum (37.84 5.12 Meckel’s diverticulum (55.24 0.37 %). decreases, indicates beginning its involution and, accordingly, formations.
Язык: Английский
Процитировано
2
Tissue Engineering and Regenerative Medicine, Год журнала: 2023, Номер 20(3), С. 325 - 327
Опубликована: Апрель 20, 2023
Язык: Английский
Процитировано
1