Update on transmission of zoonotic Giardia in cattle
Trends in Parasitology,
Год журнала:
2025,
Номер
41(3), С. 210 - 221
Опубликована: Янв. 31, 2025
Язык: Английский
Metabarcoding captures genetic diversity and links cases in outbreaks of cryptosporidiosis in New Zealand
Journal of Infection,
Год журнала:
2025,
Номер
90(2), С. 106427 - 106427
Опубликована: Янв. 29, 2025
Cryptosporidiosis
is
a
disease
caused
by
the
parasite
Cryptosporidium.
Globally,
it
leading
cause
of
diarrhoea
and
notifiable
in
New
Zealand.
Molecular
analyses
Cryptosporidium
isolated
from
notified
cases
do
not
always
provide
support
for
epidemiological
links
between
individuals.
We
hypothesised
this
could
be
due
to
undetected
diversity
use
consensus
Sanger
sequence
analyses.
Here,
we
analysed
105
samples
outbreaks
sporadic
occurring
2010
2018
Zealand
using
both
Next-Generation
Sequencing
(NGS)
sequencing
glycoprotein
60
(gp60)
locus.
NGS
metabarcoding
at
gp60
locus
uncovered
significant
intra-
inter-sample
genotypic
identified
subtypes
shared
epidemiologically
linked
cases,
along
with
rare
subtypes,
suggesting
may
useful
tool
investigations.
Язык: Английский
Multicopy subtelomeric genes underlie animal infectivity of divergent Cryptosporidium hominis subtypes
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Дек. 30, 2024
The
anthroponotic
Cryptosporidium
hominis
differs
from
the
zoonotic
C.
parvum
in
its
lack
of
infectivity
to
animals,
but
several
divergent
subtypes
have
recently
been
found
nonhuman
primates
and
equines.
Here,
we
sequence
17
animal
isolates
generate
a
new
IbA12G3
genome
at
chromosome
level.
Comparative
analysis
with
222
human
shows
significant
genetic
divergence
isolates,
recombination
among
them.
They
additional
subtelomeric
insulinase
MEDLE
genes.
In
interferon-γ
knockout
mice,
three
monkey
show
differences
induce
higher
longer
oocyst
shedding
than
reference
isolate.
Deletion
genes
significantly
reduces
growth
pathogenicity
virulent
strain
mice.
Co-infection
two
fluorescence-tagged
produces
bicolored
oocysts,
supporting
conclusion
that
mixed
subtype
infections
can
lead
recombination.
These
data
provide
insight
into
potential
determinants
host
Cryptosporidium,
convenient
model
for
biological
studies
hominis.
Язык: Английский
A new chromosome-level genome assembly and annotation of Cryptosporidium meleagridis
Scientific Data,
Год журнала:
2024,
Номер
11(1)
Опубликована: Дек. 18, 2024
Cryptosporidium
spp.
are
medically
and
scientifically
relevant
protozoan
parasites
that
cause
severe
diarrheal
illness
in
infants,
immunosuppressed
populations
many
animals.
Although
most
human
infections
caused
by
C.
parvum
hominis,
there
several
other
human-infecting
species
including
meleagridis,
which
commonly
observed
developing
countries.
Here,
we
annotated
a
hybrid
long-read
Oxford
Nanopore
Technologies
short-read
Illumina
genome
assembly
for
meleagridis
(CmTU1867)
with
DNA
generated
using
multiple
displacement
amplification.
The
was
then
compared
to
the
previous
(CmUKMEL1)
annotation
recent
telomere-to-telomere
assembly.
chromosome-level
is
9.2
Mb
contig
N50
of
1.1
Mb.
Annotation
revealed
3,919
protein-encoding
genes.
A
BUSCO
analysis
indicates
completeness
96.6%.
new
contains
166
additional
genes
reveals
high
synteny
IOWA
II
(CpBGF).
nearly
gap-free
provides
valuable
resource
community
future
studies
on
evolution
host-specificity.
Язык: Английский
Bacterial and Parasitic Characterization of the Rivers in Cuenca, Ecuador
Water,
Год журнала:
2024,
Номер
16(14), С. 2016 - 2016
Опубликована: Июль 16, 2024
Cryptosporidium
and
Giardia
are
infectious
parasitic
forms
widely
distributed
in
aquatic
ecosystems
resistant
to
disinfection
of
drinking
water.
Their
presence
was
investigated
the
lower
areas
city’s
four
rivers
through
a
four-stage
methodology.
Between
December
2017
April
2018,
three
monitoring
campaigns
were
conducted,
with
results
ranging
between
not
detected
500
oocysts/L
for
Cryptosporidium,
300
Giardia.
more
abundant,
especially
Machángara
River.
In
same
period,
bacteriological
quality
also
reviewed
using
Total
Streptococci
Fecal
Enterococci
expressed
colony-forming
units
(CFU)/100
mL
as
indicators.
The
showed
progressive
increase
pollution
course
progressed.
sensitivity
bacterial
indicators
changes
is
observed,
which
why
their
use
specific
studies
recommended.
It
concluded
that
untreated
domestic
wastewater
discharges
may
be
main
source
contamination
by
bacteria
parasites
there
relationship
concentration
seasonal
period.
dry
weather,
higher
both
microorganisms.
This
study
fills
gap
knowledge
region,
due
absence
data
on
great
impacts
public
health.
Язык: Английский