Facile Access to Branched Multispecific Proteins DOI Creative Commons
Aniekan Okon,

Jin-Yi Yang,

JoLynn B. Giancola

и другие.

Bioconjugate Chemistry, Год журнала: 2024, Номер 35(7), С. 954 - 962

Опубликована: Июнь 16, 2024

Approaches that leverage orthogonal chemical reactions to generate protein–protein conjugates have expanded access bespoke chimeras. Although the literature is replete with examples of semisynthesis bispecific proteins, few methods exist for protein higher complexity (i.e., greater than two-protein fusions). The recent emergence trispecific cell engagers immune redirection therapies necessitates development construction proteins would otherwise be inaccessible via natural synthesis. Here, we demonstrate 3-bromo-5-methylene pyrrolone (3Br-5MP) can used effect facile synthesis peptides and exquisite control over addition each monomer. multimeric complexes maintain epitope functionality both in human cells upon immobilization. We anticipate using this 3Br-5MP will broad utility basic science research quicken pace establish novel, therapies.

Язык: Английский

Ultrafast Au(III)-Mediated Arylation of Cysteine DOI
Evan A. Doud, James A. R. Tilden, Joseph W. Treacy

и другие.

Journal of the American Chemical Society, Год журнала: 2024, Номер 146(18), С. 12365 - 12374

Опубликована: Апрель 24, 2024

Through mechanistic work and rational design, we have developed the fastest organometallic abiotic Cys bioconjugation. As a result, Au(III) bioconjugation reagents enable selective labeling of moieties down to picomolar concentrations allow for rapid construction complex heterostructures from peptides, proteins, oligonucleotides. This showcases how chemistry can be interfaced with biomolecules lead range reactivities that are largely unmatched by classical organic tools.

Язык: Английский

Процитировано

17
Organometallic Oxidative Addition Complexes for S-Arylation of Free Cysteines DOI
Hayden R. Montgomery, Alexander M. Spokoyny, Heather D. Maynard

и другие.

Bioconjugate Chemistry, Год журнала: 2024, Номер 35(7), С. 883 - 889

Опубликована: Июнь 24, 2024

Development of bioconjugation strategies to efficiently modify biomolecules is key importance for fundamental and translational scientific studies. Cysteine

Язык: Английский

Процитировано

5
Harnessing the hydrolytic stability of poly(2-oxazine) living chain-ends for direct aqueous bioconjugations DOI
Niccolò Lusiani, Ondřej Sedláček

European Polymer Journal, Год журнала: 2025, Номер unknown, С. 113842 - 113842

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Post-synthetic Chemical Functionalization of Peptides DOI

Stephanie A. Barros,

Rosaura Padilla‐Salinas, Irini Abdiaj

и другие.

Elsevier eBooks, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Study of uricase-polynorbornene conjugates derived from grafting-from ring-opening metathesis polymerization DOI

Elizabathe Davis,

Adam A. Caparco, Elizabeth R. Jones

и другие.

Journal of Materials Chemistry B, Год журнала: 2024, Номер 12(8), С. 2197 - 2206

Опубликована: Янв. 1, 2024

Grafting-from ROMP-derived polynorbornene-based UO conjugates retain bioactivity, improves stability, and evades anti-PEG recognition could be a potential PEG alternative.

Язык: Английский

Процитировано

2
Facile Access to Branched Multispecific Proteins DOI Creative Commons
Aniekan Okon,

Jin-Yi Yang,

JoLynn B. Giancola

и другие.

Bioconjugate Chemistry, Год журнала: 2024, Номер 35(7), С. 954 - 962

Опубликована: Июнь 16, 2024

Approaches that leverage orthogonal chemical reactions to generate protein–protein conjugates have expanded access bespoke chimeras. Although the literature is replete with examples of semisynthesis bispecific proteins, few methods exist for protein higher complexity (i.e., greater than two-protein fusions). The recent emergence trispecific cell engagers immune redirection therapies necessitates development construction proteins would otherwise be inaccessible via natural synthesis. Here, we demonstrate 3-bromo-5-methylene pyrrolone (3Br-5MP) can used effect facile synthesis peptides and exquisite control over addition each monomer. multimeric complexes maintain epitope functionality both in human cells upon immobilization. We anticipate using this 3Br-5MP will broad utility basic science research quicken pace establish novel, therapies.

Язык: Английский

Процитировано

1