Bacillus proteolyticus UPMC1508: A novel bacterial strain capable of biologically synthesize iron oxide nanoparticles DOI

Yusur Ramzi Hasan,

Fadzlie Wong Faizal Wong, Murni Halim

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 5, 2024

Abstract Antibiotic resistance is a growing concern due to the overuse of antibiotics. Alternative treatments, such as nanoparticles, are being explored. Biological synthesis iron oxide nanoparticles (Fe3O4-NPs) via probiotics offers sustainable and cost-effective method over toxic chemical approaches, but there challenges regarding its heavy metal toxicity obtained nanoparticles. Thus, this research aims biologically synthesize Fe₃O₄-NPs new bacterial isolate evaluate toxicity. The objectives characterize novel with probiotic potential. Then, Fe3O4-NPs Transmission Electron Microscopy (TEM), Field Emission Scanning (FE-SEM), Powder Diffraction Techniques (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), nanosizer. Finally, cytotoxicity potential MTT– (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. A total 12 samples were collected from six different sites in Selangor, Malaysia. 16s rRNA sequencing came closest Bacillus proteolyticus UPMC1508 (99.87%). This strain has lower antibiotic high Fe-tolerance (MTC = 0.8 mg.mL− 1). It successfully synthesized Fe3O4-NPs, which exhibited absorption curves between 290–300 nm. TEM FE-SEM indicated spherical formed Fe3O4-NPs; average diameter was 5.12 ± 0.95 Meanwhile, XRD peaks revealed that grain size around 32.61 nanosizer hydrodynamic 104 nm good Polydispersity index (PDI) value (0.217). FT-IR satisfactory stability after 2 months. showed low at 0.031 1.The findings isolated B. Fe-heavy tolerance less resistance. Furthermore, it safety, making them suitable for therapeutic platforms, antibacterial anticancer. significance study lies offering an eco-friendly, low-cost approach while expanding applicability safe biomedical applications.

Язык: Английский

Eco-friendly Synthesis of Iron Oxide Nanoparticles Using Parietaria alsinifolia Extracts and Evaluation of Biological Applications DOI Creative Commons

Zakir Ullah,

Javed Iqbal, Bilal Haider Abbasi

и другие.

Applied Biochemistry and Biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 3, 2025

Язык: Английский

Процитировано

1
Bacillus proteolyticus UPMC1508: A novel bacterial strain capable of biologically synthesize iron oxide nanoparticles DOI
Rosfarizan Mohamad, Fadzlie Wong Faizal Wong, Siti Efliza Ashari

и другие.

Biologia, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Язык: Английский

Процитировано

0
Exploration of Novel Antimicrobial Agents against Foodborne Pathogens via a Deep Learning Approach DOI

Huixi Zhang,

Siyu Jiang,

Haishu Sun

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 13, 2025

The emergence of antibiotic-resistant bacteria poses a severe threat to food safety and human health, necessitating an urgent search for novel antimicrobial agents that can be applied in the industry. This study utilizes deep learning approach establish optimal models antibacterial activity against foodborne pathogens, particularly Escherichia coli Staphylococcus aureus, as well predicting carcinogenicity. These are screen natural products from COCONUT database, resulting identification 130 compounds with both noncarcinogenic properties. Two products, bis(hexamethylene)triamine N-phenethylbiguanide, selected experimental validation their activity. confirmation properties validates reliability developed this study. By providing innovative identifying research offers new insights discovering effective antimicrobials efficient manner.

Язык: Английский

Процитировано

0
Bacillus proteolyticus UPMC1508: A novel bacterial strain capable of biologically synthesize iron oxide nanoparticles DOI

Yusur Ramzi Hasan,

Fadzlie Wong Faizal Wong, Murni Halim

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 5, 2024

Abstract Antibiotic resistance is a growing concern due to the overuse of antibiotics. Alternative treatments, such as nanoparticles, are being explored. Biological synthesis iron oxide nanoparticles (Fe3O4-NPs) via probiotics offers sustainable and cost-effective method over toxic chemical approaches, but there challenges regarding its heavy metal toxicity obtained nanoparticles. Thus, this research aims biologically synthesize Fe₃O₄-NPs new bacterial isolate evaluate toxicity. The objectives characterize novel with probiotic potential. Then, Fe3O4-NPs Transmission Electron Microscopy (TEM), Field Emission Scanning (FE-SEM), Powder Diffraction Techniques (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), nanosizer. Finally, cytotoxicity potential MTT– (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. A total 12 samples were collected from six different sites in Selangor, Malaysia. 16s rRNA sequencing came closest Bacillus proteolyticus UPMC1508 (99.87%). This strain has lower antibiotic high Fe-tolerance (MTC = 0.8 mg.mL− 1). It successfully synthesized Fe3O4-NPs, which exhibited absorption curves between 290–300 nm. TEM FE-SEM indicated spherical formed Fe3O4-NPs; average diameter was 5.12 ± 0.95 Meanwhile, XRD peaks revealed that grain size around 32.61 nanosizer hydrodynamic 104 nm good Polydispersity index (PDI) value (0.217). FT-IR satisfactory stability after 2 months. showed low at 0.031 1.The findings isolated B. Fe-heavy tolerance less resistance. Furthermore, it safety, making them suitable for therapeutic platforms, antibacterial anticancer. significance study lies offering an eco-friendly, low-cost approach while expanding applicability safe biomedical applications.

Язык: Английский

Процитировано

0