Biodegradation of insecticides: oligonucleotide insecticides and double-stranded RNA biocontrols paving the way for eco-innovation
Frontiers in Environmental Science,
Год журнала:
2024,
Номер
12
Опубликована: Окт. 23, 2024
Each
new
class
of
insecticides
that
emerged
during
the
development
plant
protection
gradually
found
most
suitable
group
insect
pests
for
application.
At
same
time,
each
individual
insecticide,
a
balance
was
sought
between
its
effectiveness,
on
one
hand,
and
safety
non-target
organisms
ecosystem
as
whole,
other
hand.
Neonicotinoids,
diamides
pyrethroids,
effective
control
agents,
dominate
insecticide
market,
but
do
not
have
outstanding
performance
in
selectivity
biodegradation.
The
biodegradation
is
important
indicators,
representing
what
will
be
said
about
hidden
costs
resulting
harvest
paid
by
environment
human
health.
Oligonucleotide
(contact
unmodified
antisense
DNA
(CUAD)
biotechnology,
or
‘genetic
zipper’
method)
RNA
biocontrols
(double-stranded
technology)
natural
polymers
next-generation
classes
possess
unique
characteristics
fast
high
action.
While
current
chemical
require
days,
months
even
years
bacteria
fungi,
oligonucleotide
are
substantially
biodegraded
within
hours
presence
nucleases.
Nucleic
acid-based
potential
to
complement
existing
market
set
an
eco-precedent
crop
products
where
effectiveness
determined
organisms,
factors
being
equal,
choice
particular
agent
biodegradability.
It
should
noted
single
once
appeared
has
completely
disappeared;
rather,
it
occupied
niche,
declining
under
pressure
insecticides.
common
trend
towards
use
with
higher
biodegradability,
which
gives
hope
safer
future
planet.
Язык: Английский
rRNA-specific antisense DNA and dsDNA trigger rRNA biogenesis and cause potent insecticidal effect on insect pestCoccus hesperidumL.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 17, 2024
Abstract
As
a
completely
new
principle
and
active
substance
for
plant
protection,
unmodified
DNA
was
shown
to
function
as
contact
insecticide
in
2008.
CUAD
(contact
antisense
DNA)
biotechnology
is
built
on
the
use
of
short
oligonucleotides
insect
pest
control.
Being
novel
class
insecticides,
oligonucleotide
insecticides
target
rRNAs
and/or
pre-rRNAs
recently
showed
high
insecticidal
potential
against
sap-feeding
pests,
main
vectors
viruses
one
most
economically-damaging
groups
herbivorous
insects.
In
order
all
possible
opportunities
biotechnology,
this
article
we
investigated
11-mer
oligos
comparison
with
long
56-mer
single-stranded
double-stranded
sequences
Coccus
hesperidum
control
found
lower
efficiency
latter.
Also
show
that
containment
(DNAc)
mechanism,
sternorrhynchans,
represents
interesting
important
cell
life
interplay
between
different
types
oligos.
course
DNAc,
hypercompensation
rRNA
triggered
by
highly
somewhat
complementary
but
more
pronounced
later
degradation
significant
mortality
seen
only
case
perfect
complementarity
rRNA.
Oligonucleotide
are
effective
safe
agents
sternorrhynchans
other
easy
‘tune’
particular
crops
also
flexible
instantly
re-create
target-site
resistance.
Minimalist
approach,
dissolved
water,
so
potent
selective
eco-friendly
innovation
reveals
entirely
dimension
protection
–
DNA-programmable
Fundamentally
important,
surprising
results
reveal
regulation
expression
via
interaction
viral
DNA.
Significance
Statement
Using
platform
any
farmer,
botanical
garden
or
company
capable
creating
its
own
unique
arsenal
well-tailored
population
pest.
Most
technological
innovations
start
out
very
expensive
don’t
work
well,
eventually
working
well
become
cheap.
close
point
be
implemented
at
affordable
price
certain
pests
large
scale.
Results
provide
knowledge
about
host
sheds
light
Язык: Английский
The 'genetic zipper' method offers a cost-effective solution for aphid control
Frontiers in Insect Science,
Год журнала:
2024,
Номер
4
Опубликована: Дек. 11, 2024
Twenty
years
ago,
it
was
difficult
to
imagine
the
use
of
nucleic
acids
in
plant
protection
as
insecticides,
but
today
is
a
reality.
New
technologies
often
work
inefficiently
and
are
very
expensive;
however,
qualitative
changes
occur
during
their
development,
making
them
more
accessible
effectively.
Invented
2008,
contact
oligonucleotide
insecticides
(olinscides,
or
DNA
insecticides)
based
on
CUAD
(contact
unmodified
antisense
DNA)
platform
have
been
substantially
improved
rethought.
The
main
paradigm
shift
demonstrating
that
can
act
insecticide.
Key
breakthroughs
included
identifying
convenient
target
genes
(rRNA
genes),
mechanism
action
(DNA
containment),
discovering
insect
pests
(sternorrhynchans)
with
high
susceptibility
olinscides.
Today,
possesses
impressive
characteristics:
low
carbon
footprint,
safety
for
non-target
organisms,
rapid
biodegradability,
avoidance
target-site
resistance.
This
next-generation
class
creates
opportunities
developing
products
tailored
specific
pest
populations.
‘genetic
zipper’
method,
biotechnology,
integrates
molecular
genetics,
bioinformatics,
vitro
acid
synthesis.
It
serves
simple
flexible
tool
DNA-programmable
using
oligonucleotides
targeting
rRNAs.
Aphids,
key
important
agricultural
crops,
be
effectively
controlled
by
at
an
affordable
price,
ensuring
efficient
control
minimal
environmental
risks.
In
this
article,
low-dose
concentration
(0.1
ng/µL;
20
mg
per
hectare
200
L
water)
11
nt
long
insecticide
Schip-11
shows
effectiveness
against
aphid
Schizolachnus
pineti
,
causing
mortality
rate
76.06
±
7.68
12
th
day
(p<0.05).
At
consumption
hectare,
cost
required
about
0.5
USD/ha
liquid-phase
synthesis
competitive
market
lab
investigations.
We
also
show
non-canonical
base
pairing
G
olinscide
:
U
rRNA
well
tolerated
aphids.
Thus,
base-pairing
should
considered
not
harm
organisms
easily
solved
design
insecticides.
helps
quickly
create
plethora
pesticides
aphids
other
pests.
Already
today,
according
our
estimations,
potentially
capable
controlling
10-15%
all
algorithm.
Язык: Английский