Isobolographic interactions of cannabidiol and AM 1172 with cisplatin in human neuroblastoma and glioblastoma cell lines: an in vitro study
Chemico-Biological Interactions,
Год журнала:
2025,
Номер
unknown, С. 111392 - 111392
Опубликована: Янв. 1, 2025
Язык: Английский
Cannabinoids Activate Endoplasmic Reticulum Stress Response and Promote the Death of Avian Retinal Müller Cells in Culture
Brain Sciences,
Год журнала:
2025,
Номер
15(3), С. 291 - 291
Опубликована: Март 10, 2025
Background/Objectives:
Activation
of
cannabinoid
CB1
or
CB2
receptors
induces
the
death
glial
progenitors
from
chick
retina
in
culture.
Here,
by
using
an
enriched
retinal
cell
culture,
we
characterized
some
mechanisms
underlying
promoted
cannabinoids.
Methods
and
Results:
Retinal
cultures
obtained
8-day-old
(E8)
embryos
maintained
for
12–15
days
(C12–15)
were
used.
MTT
assays
revealed
that
CB1/CB2
agonist
WIN
55,212-2
(WIN)
decreased
viability
a
time-dependent
manner,
with
concomitant
increase
extracellular
LDH
activity,
suggesting
membrane
integrity
loss.
Cell
was
also
dose-dependently
induced
cannabidiol
(CBD),
Δ9-tetrahydrocannabinol
(THC),
CP55940,
another
agonist.
In
contrast
to
WIN-induced
not
blocked
either
antagonist,
deleterious
effect
CBD
receptor
antagonist
SR144528,
but
PF514273,
antagonist.
WIN-treated
showed
cells
large
vacuoles
cytoplasm
absent
incubated
plus
4-phenyl-butyrate
(PBA),
chemical
chaperone.
Since
cannabinoids
phosphorylation
eukaryotic
initiation
factor
2-alfa
(eIF2α),
these
results
suggest
process
endoplasmic
reticulum
(ER)
swelling
stress.
Incubation
4
h
~five-fold
number
labeled
ROS
indicator
CM-H2DCFDA.
JNK
p38
cultures,
expressing
cleaved-caspase
3
(c-CASP3).
The
decrease
expression
c-CASP3
salubrinal,
inhibitor
eIF2α
dephosphorylation.
Conclusions:
These
data
induce
apoptosis
culture
promoting
production,
ER
stress,
phosphorylation,
caspase-3
processing.
graphical
abstract
created
at
Biorender.com.
Язык: Английский
Antiproliferative effects of arvanil, olvanil and LY2183240 on human neuroblastoma and glioblastoma cell lines and their in vitro interactions with cisplatin and temozolomide: an isobolographic analysis
European Journal of Pharmacology,
Год журнала:
2025,
Номер
1001, С. 177775 - 177775
Опубликована: Май 27, 2025
Язык: Английский
How Biodegradable Polymers Can be Effective Drug Delivery Systems for Cannabinoids? Prospectives and Challenges
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 4607 - 4649
Опубликована: Май 1, 2024
Abstract:
Cannabinoids
are
compounds
found
in
and
derived
from
the
Cannabis
plants
that
have
become
increasingly
recognised
as
significant
modulating
factors
of
physiological
mechanisms
inflammatory
reactions
organism,
thus
inevitably
affecting
maintenance
homeostasis.
Medical
popularity
has
surged
since
its
legal
regulation
growing
around
world.
Numerous
promising
discoveries
bring
more
data
on
cannabinoids'
pharmacological
characteristics
therapeutic
applications.
Given
current
surge
interest
medical
use
cannabinoids,
there
is
an
urgent
need
for
effective
method
their
administration.
Surpassing
low
bioavailability,
water
solubility,
instability
became
important
milestone
advancement
cannabinoids
pharmaceutical
The
numerous
uses
clinical
practice
remain
restricted
by
limited
administration
alternatives,
but
hope
when
biodegradable
polymers
taken
into
account.
primary
objective
this
review
to
highlight
wide
range
indications
which
may
be
used,
well
polymeric
carriers
enhance
effectiveness.
described
a
applications
including
pain
management,
neurological
sleep
disorders,
anxiety,
cancer
treatment.
these
was
further
examined
area
dermatology
cosmetology.
Finally,
with
polymer-based
drug
delivery
systems
(DDSs),
it
demonstrated
can
delivered
specifically
intended
site
while
also
improving
drug's
physicochemical
properties,
emphasizing
utility.
Nevertheless,
additional
trials
novel
formulations
required,
full
spectrum
therapeutical
potential
yet
unravelled.
Keywords:
systems,
cannabidiol,
tetrahydrocannabinol,
polymers,
nanomedicine
Язык: Английский
Cannabinoid-Induced Immunogenic Cell Death of Colorectal Cancer Cells Through De Novo Synthesis of Ceramide Is Partially Mediated by CB2 Receptor
Cancers,
Год журнала:
2024,
Номер
16(23), С. 3973 - 3973
Опубликована: Ноя. 27, 2024
Background:
Our
recent
studies
have
identified
a
link
between
sphingolipid
metabolites
and
the
induction
of
specialized
form
regulated
cell
death
termed
immunogenic
(ICD).
We
recently
demonstrated
that
synthetic
cannabinoid
(±)
5-epi
CP
55,940
(5-epi)
stimulates
accumulation
ceramide
(Cer),
inhibition
sphingosine
kinase
1
(SphK1)
enhances
Cer
ICD-induction
in
human
colorectal
cancer
(CRC)
lines.
Methods:
employed
flow-cytometric,
western
blot
analyses,
pharmacological
inhibitors
metabolic
pathway
small
molecule
agonists
antagonists
CB
receptors
to
further
analyze
mechanism
by
which
induces
accumulation.
Results:
Herein,
report
de
novo
synthesis
primarily
through
engagement
receptor
2
(CB2)
depletion
intracellular
calcium
levels.
Moreover,
we
dysregulation
endogenous
inhibitor
pathway,
ORMDL3.
also
observed
remarkable
specific
one
species,
C20:4
Cer,
generated
predominantly
synthase
4,
as
key
factor
required
for
5-epi-induced
ICD.
Conclusions:
Together,
these
data
indicate
CB2,
5-epi,
alters
regulation
generate
species
mediate
Язык: Английский
Improving the Biopharmaceutical Properties of Cannabinoids in Glioblastoma Multiforme Therapy With Nanotechnology: A Drug Delivery Perspective
Drug Development Research,
Год журнала:
2024,
Номер
85(8)
Опубликована: Дек. 1, 2024
ABSTRACT
Glioblastoma
multiforme
(GBM)
is
the
most
prevalent
primary
brain
tumor
in
adults
and
known
for
its
rapid
proliferation
infiltrative
nature.
Current
therapeutic
strategies
include
surgical
resection
followed
by
radio‐
chemotherapy.
Still,
they
are
hindered
GBM
biological
characteristics
physical‐chemical
properties
of
chemotherapeutic
drugs,
leading
to
limited
efficacy
poor
prognosis.
Cannabinoids
have
emerged
as
potential
anti‐GBM
agents,
exhibiting
antiangiogenic,
antimetastatic,
antiproliferative
effects.
However,
their
hydrophobicity
oral
bioavailability
pose
significant
challenges
clinical
applications.
This
study
evaluates
nanocarriers
enhancing
solubility
targeted
delivery
cannabinoids
therapy.
The
innovative
combination
nanotechnology
with
cannabinoid‐based
treatment
offers
a
promising
strategy
improve
outcomes.
We
addressed
application
deliver
cannabinoids,
which
can
enhance
passage
across
blood‐brain
barrier
enable
Studies
demonstrate
improving
solubility,
stability,
controlled
release
highlighting
advancements
nanocarrier
design
optimized
glioma
cells.
exert
antitumor
effect,
including
induction
apoptosis
through
ceramide
p8‐regulated
pathways
modulation
immune
responses.
evidence
found
this
supports
nanotechnologies
regimens
patient
Язык: Английский