An autonomous robotic system for high-throughput phenotyping and behavioral studies of individual fruit flies DOI Creative Commons
Seung Je Woo, Cheng Huang, Joan Savall

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 22, 2024

The fruit fly, Drosophila melanogaster, is a widely used model species in biomedical research. Despite its importance, conducting manual experiments with individual flies can be challenging and time-consuming, especially for studies of fly behaviors. Such often involve cumbersome preparatory steps, such as manually tethering then positioning it within an experimental setup1,2. These procedures commonly require the to anesthetized, and, before behavioral assessments begin, must recover from anesthesia. Hence, introduction automated phenotyping assays would expedite important aspects research, by minimizing handling decreasing net time needed experiments. Here, we introduce FlyMAX (Fly Manipulation Autonomous eXperimentation), autonomous robotic system manipulating adult without use collects standard vial, analyzes them computer vision, achieves throughput >1,000 per day high-throughput inspection characterization assays. Robotic had no detectable adverse effects on longevity or our health. Moreover, performance flies, males, was better less variable than handled manually. Our employs deep learning-based machine vision real-time picking quality phenotypes. This enables fully pipelined, experimentation controlled environments, which previously infeasible. Overall, constitutes promising technology enhance efficiency reproducibility research other insects fields genetics, neuroscience, drug screening.

Язык: Английский

Effects of RDL GABA Receptor Point Mutants on Susceptibility to Meta-Diamide and Isoxazoline Insecticides in Drosophila melanogaster DOI Creative Commons

Tian-Hao Zhou,

Weiping Wu,

Suhan Ma

и другие.

Insects, Год журнала: 2024, Номер 15(5), С. 334 - 334

Опубликована: Май 6, 2024

Ionotropic γ-aminobutyric acid (GABA) receptors in insects, specifically those composed of the RDL (resistant to dieldrin) subunit, serve as important targets for commonly used synthetic insecticides. These insecticides belong various chemical classes, such phenylpyrazoles, cyclodienes, meta-diamides, and isoxazolines, with latter two potentially binding transmembrane inter-subunit pocket. However, specific amino residues that contribute high sensitivity insect these novel remain elusive. In this study, we investigated susceptibility seven distinct Drosophila melanogaster Rdl point mutants against four meta-diamide isoxazoline insecticides: isocycloseram, fluxametamide, fluralaner, broflanilide. Our findings indicate that, despite exhibiting increased fluralaner vitro, RdlI276C mutant showed resistance isocycloseram fluxametamide. Similarly, double-points RdlI276F+G279S also decreased tested isoxazolines. On other hand, RdlG335M displayed levels all Molecular modeling docking simulations further supported findings, highlighting similar poses summary, our research provides robust vivo evidence supporting idea acids within M1 M3 domains form site crucial insecticide interactions. This study highlights complex interplay between mutations susceptibility, paving way more targeted pest control strategies.

Язык: Английский

Процитировано

5
How to Identify Pesticide Targets? DOI
Ziyu Zhang, Ferdinand Ndikuryayo, Jungang Wang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Pesticides are essential in contemporary agriculture, as they improve crop yields and quality while safeguarding against pests. However, long-term heavy use of traditional pesticides has led to increased pest resistance, these often toxic less selective, may also have adverse effects on the environment nontarget organisms. To solve this problem, it is important find new targets for pesticide develop more effective environmentally friendly alternatives. Therefore, exploring action become one current research focuses. In past years, efforts been made investigate possible strategies, work systematically summarizes criticizes most recently used ones. This contribution not only helps promote development but holds substantial implications promoting sustainable agriculture food security.

Язык: Английский

Процитировано

0
A key amino acid substitution of vacuolar-type H+-ATPases A subunit (VATP-A) confers selective toxicity of a potential botanical insecticide, periplocoside P (PSP), in Mythimna separata and Spodoptera exigua DOI
Xianxia Zhang, Yayun Zuo, Rui Liu

и другие.

Insect Biochemistry and Molecular Biology, Год журнала: 2025, Номер unknown, С. 104277 - 104277

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Taste Detection of Flonicamid in Drosophila melanogaster DOI
Muhammad Atif, Youngseok Lee

Insect Biochemistry and Molecular Biology, Год журнала: 2025, Номер unknown, С. 104302 - 104302

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Investigating the neuroprotective effects of strawberry extract against diesel soot-induced motor dysfunction in Drosophila: an in-vivo and in-silico study DOI

A Ravindranath,

Avula Muralidhar,

Nikki Gambhir

и другие.

In Silico Pharmacology, Год журнала: 2025, Номер 13(2)

Опубликована: Апрель 16, 2025

Язык: Английский

Процитировано

0
Multiple Mutations in the β1 Subunit of the Nicotinic Acetylcholine Receptor Confer Resistance to Neonicotinoids DOI
Yang Cheng, Yuxin Chen, J. R. Gao

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 12, 2025

The evolution of resistance to neonicotinoid insecticides threatens global agriculture. To elucidate its molecular basis, we employed Drosophila melanogaster as a model system investigate resistance-associated mutations in the β1 subunit nicotinic acetylcholine receptors (nAChRs). Using CRISPR/Cas9-mediated allele replacement, generated homozygous knock-in mutants (V62I, V101I, R81E, and A60T,R81E) without apparent fitness costs. Toxicity bioassays revealed that these confer varying levels, with R81E mutation exhibiting over 225-fold thiamethoxam, clothianidin, dinotefuran. A heteropentameric α1β1 nAChR model, using an AI-based protein-ligand prediction (Chai-1), showed imidacloprid binds at orthosteric site, where R81 forms critical electrostatic interaction. Residues A60, V62, V101, positioned further from binding spatial distances correlated their ratios. These findings provide genetic structural insights into mechanisms, offering foundation for design next-generation management strategies.

Язык: Английский

Процитировано

0
Effects of Lithium on Mortality and Metabolite Profiles in Drosophila Lithium-Inducible SLC6 Transporter Mutants DOI

Junko Kasuya,

Karina Kruth, Dong-Keun Lee

и другие.

Environmental Toxicology and Pharmacology, Год журнала: 2025, Номер unknown, С. 104684 - 104684

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Dietary acrylamide disrupts the functioning of the biological clock DOI Creative Commons
Jolanta Górska‐Andrzejak,

Lucyna Widacha,

Robert Wadowski

и другие.

Journal of Hazardous Materials, Год журнала: 2024, Номер 476, С. 134912 - 134912

Опубликована: Июнь 13, 2024

Acrylamide (ACR) is a known carcinogen and neurotoxin. It chronically consumed in carbohydrate-rich snacks processed at high temperatures. This calls for systematic research into the effects of ACR intake, best performed an experimental model capable detecting symptoms its neurotoxicity both low doses. Here, we study influence 10 µg/g (corresponding to concentrations found food products) and, comparison, 60, 80 110 dietary ACR, on fruit fly Drosophila melanogaster. We show that chronic administration affects lifespan, activity level most importantly, daily circadian pattern locomotor Drosophila. ACR-treated flies well-defined concentration-dependent neurotoxicity; reduced anticipation upcoming changes light conditions increased arrhythmicity constant darkness. The results suggest rhythm-generating neural circuits their oscillator (biological clock) are sensitive even if exposure time sufficiently long. makes behavioural readout clock, rhythm activity, useful tool studying adverse probably other compounds.

Язык: Английский

Процитировано

2
Age-dependent switched taste behavior to ribose DOI
Jiun Sang, Youngseok Lee

Insect Biochemistry and Molecular Biology, Год журнала: 2024, Номер 174, С. 104194 - 104194

Опубликована: Окт. 13, 2024

Язык: Английский

Процитировано

2
An autonomous robotic system for high-throughput phenotyping and behavioral studies of individual fruit flies DOI Creative Commons
Seung Je Woo, Cheng Huang, Joan Savall

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 22, 2024

The fruit fly, Drosophila melanogaster, is a widely used model species in biomedical research. Despite its importance, conducting manual experiments with individual flies can be challenging and time-consuming, especially for studies of fly behaviors. Such often involve cumbersome preparatory steps, such as manually tethering then positioning it within an experimental setup1,2. These procedures commonly require the to anesthetized, and, before behavioral assessments begin, must recover from anesthesia. Hence, introduction automated phenotyping assays would expedite important aspects research, by minimizing handling decreasing net time needed experiments. Here, we introduce FlyMAX (Fly Manipulation Autonomous eXperimentation), autonomous robotic system manipulating adult without use collects standard vial, analyzes them computer vision, achieves throughput >1,000 per day high-throughput inspection characterization assays. Robotic had no detectable adverse effects on longevity or our health. Moreover, performance flies, males, was better less variable than handled manually. Our employs deep learning-based machine vision real-time picking quality phenotypes. This enables fully pipelined, experimentation controlled environments, which previously infeasible. Overall, constitutes promising technology enhance efficiency reproducibility research other insects fields genetics, neuroscience, drug screening.

Язык: Английский

Процитировано

0