Nature Cell Biology, Год журнала: 2024, Номер 26(10), С. 1634 - 1636
Опубликована: Окт. 1, 2024
Язык: Английский
Developmental Dynamics, Год журнала: 2025, Номер unknown
Опубликована: Март 19, 2025
Abstract Neural induction is a process by which naïve ectodermal cells differentiate into neural progenitor through the inhibition of BMP signaling, condition typically considered “default” state in vertebrate embryos. Studies embryos indicate that active FGF/MAPK signaling reduces to facilitate induction. Consequently, I propose FGF stimulation/BMP more accurately characterizes default model. Initially, neuroectoderm instructed anterior forebrain tissue, with cranial signals stabilizing this outcome. Subsequently, gradient caudalizing converts neuroectodermal posterior midbrain, hindbrain, and spinal cord. Furthermore, at caudal end embryo, neuromesodermal are destined both mesodermal cells, aiding body extension. In light these observations, suggest incorporating an additional step, elongation, conventional three‐step model This updated encompasses activation, stabilization, transformation, elongation.
Язык: Английский
Процитировано
0
Cell stem cell, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Considerable phenotypic variation under identical culture conditions limits the potential of stem-cell-based embryo models (SEMs) in basic and applied research. The biological processes causing this seemingly stochastic remain unclear. Here, we investigated roots by parallel recording transcriptomic states morphological history individual structures modeling embryonic trunk formation. Machine learning integration time-resolved single-cell RNA sequencing with imaging-based profiling identified early features predictive end states. Leveraging power revealed that imbalance oxidative phosphorylation glycolysis results aberrant morphology a neural lineage bias, which confirmed metabolic measurements. Accordingly, interventions improved Collectively, our work establishes divergent as drivers offers broadly applicable framework to chart predict organoids SEMs. strategy can be used identify control underlying processes, ultimately increasing reproducibility.
Язык: Английский
Процитировано
0
Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12
Опубликована: Ноя. 29, 2024
Early embryonic development is a complex process where undifferentiated cells lose their pluripotency and start to gastrulate. During gastrulation, three germ layers form, giving rise different cell lineages organs. This regulated by transcription factors epigenetic regulators, including non-canonical polycomb repressive 1s (ncPRC1s). Previously, we reported that ncPRC1-member RYBP (RING1 YY1 binding protein) crucial for implantation cardiac lineage commitment in mice. However, the role of gastrulation mesoderm formation has not yet been defined. In this study, used 2D 3D
Язык: Английский
Процитировано
1
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Сен. 3, 2024
Abstract Patterning of cell fates is central to embryonic development, tissue homeostasis, and disease. Quantitative analysis patterning reveals the logic by which cell-cell interactions orchestrate changes in fate. However, it challenging quantify when graded identity occur over complex 4D trajectories, or where different states are intermingled. Furthermore, comparing patterns across multiple individual embryos, tissues, organoids difficult because these often vary shape size. Here we present a toolkit computational approaches tackle problems. These strategies based on measuring properties each relation its neighbours patterning, using landmarks order compare between embryos. We use this characterise identities within caudal lateral epiblast E8.5 revealing local emergence early mesoderm cells that sensitive inhibition Notch activity.
Язык: Английский
Процитировано
0
Nature Cell Biology, Год журнала: 2024, Номер 26(10), С. 1634 - 1636
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
0