Circulating extracellular vesicles in sera of chronic patients as a method for determining active parasitism in Chagas disease DOI Creative Commons

Noelia Lozano,

Alexa Prescilla-Ledezma, Eva Muñoz

и другие.

PLoS neglected tropical diseases, Год журнала: 2024, Номер 18(11), С. e0012356 - e0012356

Опубликована: Ноя. 20, 2024

Background Chagas disease, once restricted mainly to the Americas, disease has become a global health problem due migration from endemic non-endemic areas. In regions, transmission is limited vertical infected mothers newborns or through blood and organ donations. A major challenge in management of lies diagnosis chronic cases, as blood-borne parasites are often absent antibodies persist for life, complicating evaluation treatment. Methodology main findings This study investigates whether detection circulating extracellular vesicles (EVs) their immunocomplexes with host IgGs serum patients could serve diagnostic tools biomarkers active presence parasite. method may prove valuable cases where parasitaemia other tests inconclusive, especially assessing treatment efficacy confirming mother-to-child transmission. Together exovesicle purification by ultracentrifugation, which ‘gold standard’, an affordable simplified isolation EVs was tested use less well-equipped laboratories. EV performed enzyme-linked immunosorbent assay (ELISA) targeting Trypanosoma cruzi antigens. Positive results were demonstrated Bolivian Spain, covering asymptomatic symptomatic (cardiac, gastrointestinal both). The also examined newborns. These further confirmed Panamanian inconclusive results. Moreover, IgG isotypes that formed parasite exovsicles identified, IgG2 IgG4 being predominant. Conclusions Our confirm usefulness markers metabolically T. infections without detectable parasitaemia, well tests.

Язык: Английский

Extracellular vesicles in malaria: proteomics insights, in vitro and in vivo studies indicate the need for transitioning to natural human infections DOI Creative Commons
Núria Sima, Alberto Ayllon-Hermida, Carmen Fernández-Becerra

и другие.

mBio, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

ABSTRACT Globally, an estimated 2.1 billion malaria cases and 11.7 million deaths were averted in the period 2000–2022. Noticeably, despite effective control measurements, 2022 there 249 85 malaria-endemic countries increase of 5 compared with 2021. Further understanding biology, epidemiology, pathogenesis human is therefore essential for achieving elimination. Extracellular vesicles (EVs) are membrane-enclosed nanoparticles pivotal intercellular communication secreted by all cell types. Here, we will review what currently known about EVs malaria, from biogenesis cargo to molecular insights pathophysiology. Of relevance, a meta-analysis proteomics cargo, comparisons between vitro vivo studies revealed striking differences those few reported patients. Thus, indicating need rigor standardization methodologies transitioning infections elucidate their physiological role. We conclude focus on translational aspects diagnosis vaccine development highlight key gaps knowledge research.

Язык: Английский

Процитировано

0
Spectral flow cytometry for detecting DNA cargo in malaria parasite-derived extracellular vesicles DOI Creative Commons
Ewa Kozela, Ekaterina Petrovich-Kopitman,

Yves Berger

и другие.

Journal of Biological Chemistry, Год журнала: 2025, Номер unknown, С. 108481 - 108481

Опубликована: Апрель 1, 2025

Cells across biological kingdoms release extracellular vesicles (EVs) as a means of communication with other cells, be their friends or foes. This is indeed true for the intracellular malaria parasite Plasmodium falciparum (Pf), which utilizes EVs to transport bioactive molecules various human host systems. Yet, study this mode in research currently constrained due limitations high-resolution tools and absence commercial antibodies. Here, we demonstrate power an advanced spectral flow cytometry approach robustly detect secreted EVs, isolated from Pf-infected red blood cells. By labeling both EV membrane lipids DNA cargo within (non-antibody staining approach), were able subpopulation parasitic-derived enriched DNA. Furthermore, could quantitatively measure DNA-carrying two distinct stages parasite: rings trophozoites. Our findings showcase potential monitor dynamic changes nucleic acid pathogenic EVs.

Язык: Английский

Процитировано

0
Cystoisospora suis – the non-model model coccidium DOI
Anja Joachim, Anna Sophia Feix,

Bärbel Ruttkowski

и другие.

Advances in Parasitology/Advances in parasitology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Процитировано

0
Circulating extracellular vesicles in sera of chronic patients as a method for determining active parasitism in Chagas disease DOI Creative Commons

Noelia Lozano,

Alexa Prescilla-Ledezma, Eva Muñoz

и другие.

PLoS neglected tropical diseases, Год журнала: 2024, Номер 18(11), С. e0012356 - e0012356

Опубликована: Ноя. 20, 2024

Background Chagas disease, once restricted mainly to the Americas, disease has become a global health problem due migration from endemic non-endemic areas. In regions, transmission is limited vertical infected mothers newborns or through blood and organ donations. A major challenge in management of lies diagnosis chronic cases, as blood-borne parasites are often absent antibodies persist for life, complicating evaluation treatment. Methodology main findings This study investigates whether detection circulating extracellular vesicles (EVs) their immunocomplexes with host IgGs serum patients could serve diagnostic tools biomarkers active presence parasite. method may prove valuable cases where parasitaemia other tests inconclusive, especially assessing treatment efficacy confirming mother-to-child transmission. Together exovesicle purification by ultracentrifugation, which ‘gold standard’, an affordable simplified isolation EVs was tested use less well-equipped laboratories. EV performed enzyme-linked immunosorbent assay (ELISA) targeting Trypanosoma cruzi antigens. Positive results were demonstrated Bolivian Spain, covering asymptomatic symptomatic (cardiac, gastrointestinal both). The also examined newborns. These further confirmed Panamanian inconclusive results. Moreover, IgG isotypes that formed parasite exovsicles identified, IgG2 IgG4 being predominant. Conclusions Our confirm usefulness markers metabolically T. infections without detectable parasitaemia, well tests.

Язык: Английский

Процитировано

0