Using high-dimensional immunophenotyping for mapping immune cell composition in rapid cryopreserved liquid biopsies
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 15, 2025
Abstract
Liquid
biopsies,
such
as
a
blood
sample,
are
non-invasive
and
simple
ways
to
monitor
biomarkers
in
clinical
settings.
For
cancer
immunotherapy
settings
major
goal
is
identify
host
immunological
that
can
be
used
progression
of
disease,
treatment
efficacy,
or
even
adverse
effects.
Here,
the
profiling
validation
circulating
immune
cells
may
allow
continuously
survey
on
how
system
alters
during
disease
progress
treatment.
However,
practice
will
require
proper
sampling
development
robust
high-dimensional
phenotypic
analysis.
we
designed
37-marker
spectral
flow
cytometry
assay
with
purpose
conducting
immunophenotyping
monitoring
status
patients.
The
was
developed
profile
all
cell
compartments,
their
differentiation
well
activation
markers,
including
numerous
checkpoint
markers.
Importantly,
validated
this
context
liquid
biopsies
should
processed
ensure
minimal
effect
cryopreservation
compared
freshly
acquired
sample.
Our
work
highlights
important
considerations
for
which
alternations
introduced
different
methods.
Essentially,
identified
fast
processing
pipeline
where
showed
changes
profile.
This
approach
certainly
implemented
help
diagnostic
sites
strong
tool
applicable
individual
patients’
status.
Язык: Английский
High-dimensional spectral flow cytometry of activation and phagocytosis by peripheral human polymorphonuclear leukocytes
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 5, 2024
Polymorphonuclear
lymphocytes
(PMNs)
are
terminally
differentiated
phagocytes
with
pivotal
roles
in
infection,
inflammation,
tissue
injury,
and
resolution.
PMNs
can
display
a
breadth
of
responses
to
diverse
endogenous
exogenous
stimuli,
making
understanding
these
innate
immune
responders
vital
yet
challenging
achieve.
Here,
we
report
22-color
spectral
flow
cytometry
panel
profile
primary
human
on
population
single
cell
levels
for
surface
marker
expression
activation,
degranulation,
phagocytosis,
migration,
chemotaxis,
interaction
fluorescently
labeled
cargo.
We
demonstrate
the
protein
response
phorbol
ester
stimulation
compared
untreated
controls
an
adherent
PMN
model
additional
analysis
intra-
inter-subject
variability.
challenged
Gram-negative
bacterial
pathogen
Язык: Английский