Опубликована: Март 21, 2025
Язык: Английский
Chemical Reviews, Год журнала: 2022, Номер 123(9), С. 5521 - 5570
Опубликована: Дек. 30, 2022
Metabolic engineering aims to improve the production of economically valuable molecules through genetic manipulation microbial metabolism. While discipline is a little over 30 years old, advancements in metabolic have given way industrial-level molecule benefitting multiple industries such as chemical, agriculture, food, pharmaceutical, and energy industries. This review describes design, build, test, learn steps necessary for leading successful campaign. Moreover, we highlight major applications engineering, including synthesizing chemicals fuels, broadening substrate utilization, improving host robustness with focus on specific case studies. Finally, conclude discussion perspectives future challenges related engineering.
Язык: Английский
Процитировано
111
ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 10(5), С. 2703 - 2724
Опубликована: Апрель 22, 2024
The scientific world is increasingly focusing on rare earth metal oxide nanomaterials due to their consequential biological prospects, navigated by breakthroughs in biomedical applications. Terbium belongs elements (lanthanide series) and possesses remarkably strong luminescence at lower energy emission signal transduction properties, ushering wide applications for diagnostic measurements (i.e., bioimaging, biosensors, fluorescence imaging, etc.) the sectors. In addition, theranostic of terbium-based nanoparticles further permit targeted delivery drugs specific site disease. Furthermore, antimicrobial properties terbium induced via reactive oxygen species (ROS) cause oxidative damage cell membrane nuclei living organisms, ion release, surface charge interaction, thus creating or exhibiting excellent antioxidant characteristics. Moreover, recent tissue engineering, wound healing, anticancer activity, etc., angiogenesis, proliferation, promotion growth factors, biocompatibility, cytotoxicity mitigation, anti-inflammatory potentials, make this nanoparticle anticipate a future epoch nanomaterials. stand as game changer realm research, proffering array possibilities, from revolutionary imaging techniques advanced drug systems. Their unique including luminescence, magnetic characteristics, have redefined boundaries what can be achieved biomedicine. This review primarily delves into various mechanisms involved physicochemical article explains potential with in-depth significant individual literature. additionally stands first instance furnish "single-platted" comprehensive acquaintance shaping healthcare well limitations overcoming strategies that require exploration before being trialed clinical settings.
Язык: Английский
Процитировано
21
Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Янв. 27, 2025
Visualizing mechanical stress distribution in soft and live biomaterials is essential for understanding biological processes improving material design. However, it remains challenging due to their complexity, dynamic nature, sensitivity requirements, necessitating innovative techniques. Since polysaccharides are common various biomaterials, a biosensor integrating Förster resonance energy transfer (FRET)-based tension sensor module carbohydrate-binding modules (FTSM-CBM) has been designed real-time monitoring of the these biomaterials. By simple dripping or soaking, FTSM-CBM enables fast, reproducible semiquantitative detection both 2D 3D distributions polysaccharide-based hydrogels. Additionally, provides valuable information such as microstructure hints fracture site warnings. can also monitor locomotion maggots, which not feasible with most existing Furthermore, by changing CBM, be expanded This study powerful tool that may promote related research life materials science. key understand improve Here, authors design visualize living tissues.
Язык: Английский
Процитировано
3
Coordination Chemistry Reviews, Год журнала: 2022, Номер 469, С. 214676 - 214676
Опубликована: Июнь 22, 2022
Язык: Английский
Процитировано
54
Aggregate, Год журнала: 2023, Номер 5(2)
Опубликована: Ноя. 12, 2023
Abstract Fluorogenic biosensors are essential tools widely used in biomedicine, chemical biology, environmental protection and food safety. Fluorescence resonance energy transfer (FRET) is a crucial technique for developing fluorogenic that provide mechanistic insight into bioprocesses through time‐spatial bioimaging living cells organisms. Although extensive FRET‐based sensors have been developed detecting or imaging analytes of interest over the past decade, few comprehensive reviews summarized recent studies from fundamental angle about design application. In this work, advance discovery FRET using donor‐acceptor dye combinations described they classified based on different types analytes, such as mall molecules, proteins, enzymes, nucleic acids metal ions. This review provides molecular‐level inspiration biosensors, aiding their application biosensing bioimaging.
Язык: Английский
Процитировано
35
Advanced Materials Technologies, Год журнала: 2024, Номер 9(20)
Опубликована: Май 16, 2024
Abstract The convergence of advanced nanotechnology with disease diagnosis has ushered in a transformative era healthcare, empowering early and accurate detection diseases paving the way for timely interventions, improved treatment outcomes, enhanced patient well‐being. development novel materials is frequently impetus behind significant advancements sensor technology. Among them, single‐walled carbon nanotubes (SWCNTSs) have emerged as promising nanomaterials developing biosensors. Their unique optical, electrical, biocompatibility properties make them candidates enhancing sensitivity real‐time monitoring capabilities biosensors, well enabling various bioimaging techniques. Recent studies demonstrated utility SWCNTS‐based biosensors biological analytes, such nitric oxide hydrogen peroxide (H 2 O ), potential implications understanding therapeutic response assessment. Moreover, SWCNTSs shown promise applications, including fluorescence, Raman spectroscopy, photoluminescence imaging samples. This article delves into core principles, design strategies, operational mechanisms that underpin SWCNTS‐bioimaging techniques‐based It emphasizes on their versatile functionalization nanotubes, laying foundation integration biosensor platforms applications aimed at diagnosing wide spectrum infectious diseases, cancer, neurological disorders, metabolic conditions.
Язык: Английский
Процитировано
13
Chemical Reviews, Год журнала: 2024, Номер 124(11), С. 7465 - 7530
Опубликована: Май 16, 2024
Despite their diverse functions, proteins are inherently constructed from a limited set of building blocks. These compositional constraints pose significant challenges to protein research and its practical applications. Strategically manipulating the cellular synthesis system incorporate novel blocks has emerged as critical approach for overcoming these in application. In past two decades, field genetic code expansion (GCE) achieved advancements, enabling integration numerous functionalities into across variety organisms. This technological evolution paved way extensive application multiple domains, including imaging, introduction probes research, analysis protein–protein interactions, spatiotemporal control function, exploration proteome changes induced by external stimuli, endowed with functions. this comprehensive Review, we aim provide an overview biophysical applications that have employed GCE technology over decades.
Язык: Английский
Процитировано
13
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 272, С. 132710 - 132710
Опубликована: Май 31, 2024
Язык: Английский
Процитировано
11
Micromachines, Год журнала: 2022, Номер 13(10), С. 1789 - 1789
Опубликована: Окт. 20, 2022
Resonance energy transfer technologies have achieved great success in the field of analysis. Particularly, fluorescence resonance (FRET) and bioluminescence (BRET) provide strategies to design tools for sensing molecules monitoring biological processes, which promote development biosensors. Here, we an overview recent progress on FRET- BRET-based biosensors their roles biomedicine, environmental applications, synthetic biology. This review highlights gives examples applications with strategies. The limitations future directions are also discussed.
Язык: Английский
Процитировано
31
Carbohydrate Polymers, Год журнала: 2023, Номер 307, С. 120623 - 120623
Опубликована: Янв. 25, 2023
Язык: Английский
Процитировано
20