Performance enhancement of the lateral flow immunoassay by use of composite nanoparticles as signal labels

TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 170, С. 117441 - 117441

Опубликована: Ноя. 19, 2023

Язык: Английский

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High Accuracy of Clinical Verification of Electrohydrodynamic-Driven Nanobox-on-Mirror Platform for Molecular Identification of Respiratory Viruses

Analytical Chemistry, Год журнала: 2024, Номер 96(11), С. 4495 - 4504

Опубликована: Март 6, 2024

The molecular detection of multiple respiratory viruses provides evidence for the rational use drugs and effective health management. Herein, we developed tested clinical performance an electrohydrodynamic-driven nanobox-on-mirror platform (E-NoM) parallel, accurate, sensitive four viral antigens. E-NoM uses gold–silver alloy nanoboxes as core material with deposition a silver layer shell on surfaces to amplify enable reproducible Raman signal readout that facilitates accurate detection. Additionally, employs gold microelectrode arrays mirror electrohydrodynamics manipulate fluid flow enhance interactions improved biosensing response. presence antigens binds nanobox-based core–shell nanostructure creates nanocavity extremely strong "hot spots" benefit analysis. Significantly, in large cohort 227 patients, designed demonstrates capability screening infection achieved specificity, sensitivity, accuracy 100.0, 96.48, 96.91%, respectively. It is anticipated can find position usage disease diagnosis.

Язык: Английский

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Lateral flow assays: Progress and evolution of recent trends in point-of-care applications

Materials Today Bio, Год журнала: 2024, Номер 28, С. 101188 - 101188

Опубликована: Авг. 7, 2024

Paper based point-of-care (PoC) detection platforms applying lateral flow assays (LFAs) have gained paramount approval in the diagnostic domain as well environmental applications owing to their ease of utility, low cost, and rapid signal readout. It has centralized aspect self-evaluation exhibiting promising potential last global pandemic era Covid-19 implementing management public health remote areas. In this perspective, present review is focused towards landscaping current framework LFAs along with integration components characteristics for improving assay by pushing limits. The highlights synergistic aspects designing, sample enrichment strategies, novel nanomaterials-based transducers, high-end analytical techniques that contribute significantly sensitivity specificity enhancement. Various recent studies are discussed supporting innovations LFA systems focus upon accuracy reliability PoC testing. also provides a comprehensive overview all possible difficulties commercialization subjecting its applicability pathogen surveillance, water food testing, disease diagnostics, agriculture issues.

Язык: Английский

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A Magnetic-Optical Triple-Mode Lateral Flow Immunoassay for Sensitive and Rapid Detection of Respiratory Adenovirus

Analytical Chemistry, Год журнала: 2024, Номер 96(5), С. 2059 - 2067

Опубликована: Янв. 23, 2024

Human respiratory adenovirus (ADV) is a highly infectious virus with potential for pandemics. There are currently no specific drugs to treat ADV worldwide, so early rapid detection of infection essential. In this study, we developed an innovative magnetic-optical triple-mode lateral flow immunoassay (LFIA) using magnetic quantum dots as immunomarkers. This novel approach addresses the need and accurate detection, allowing multimodal quantitative/semiquantitative analysis magnetic, fluorescent, visible signals within mere 15 min. The lower limit (LOD) visual was determined be 5.6 × 103, 1.2 1.95 104 copies/mL, respectively. range 103–5 107 copies/mL. Additionally, semiquantitative analysis, which user-friendly does not necessitate specialized equipment, successfully implemented. Notably, seven viruses showed cross-reactivity generated LFIA test strips. intrabatch repeatability exhibited coefficient variation (CV) less than 5%, while interbatch had CV 15%. Furthermore, recovery values ranged from 95% 106.8% samples analyzed concurrently dual at same spiking concentration. assay in study boasts wide exceptional sensitivity specificity. technique exceptionally well-suited on-site personal self-testing diagnosis. Its versatility extends broad array application scenarios.

Язык: Английский

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Beyond Traditional Lateral Flow Assays: Enhancing Performance Through Multianalytical Strategies

Biosensors, Год журнала: 2025, Номер 15(2), С. 68 - 68

Опубликована: Янв. 23, 2025

Multiplex lateral flow assays are one of the greatest advancements in world rapid diagnostics, achieving performance several tests one. These meet basic requirements increasing ease use, low detection limit, and high specificity, as they combine use novel strategies, such exploitation multiple labels, a variety amplification methods. have proven their usefulness many different areas, including clinical food, environmental monitoring. In this review paper, we attempt to highlight discuss predominant changes multianalyte LFAs, related principle, development, combination with other Attention is paid flexibility challenges associated LFA arrays, strategies improve detectability, sensitivity, reliability assays. Therefore, emphasizes current advances field underline possible impact multiplex LFAs on future diagnostics analytical sciences.

Язык: Английский

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Recent Advances in Lateral Flow Assays for Viral Protein Detection with Nanomaterial-Based Optical Sensors

Biosensors, Год журнала: 2024, Номер 14(4), С. 197 - 197

Опубликована: Апрель 17, 2024

Controlling the progression of contagious diseases is crucial for public health management, emphasizing importance early viral infection diagnosis. In response, lateral flow assays (LFAs) have been successfully utilized in point-of-care (POC) testing, emerging as a viable alternative to more traditional diagnostic methods. Recent advancements virus detection primarily leveraged methods such reverse transcription–polymerase chain reaction (RT-PCR), transcription–loop-mediated isothermal amplification (RT-LAMP), and enzyme-linked immunosorbent assay (ELISA). Despite their proven effectiveness, these conventional techniques are often expensive, require specialized expertise, consume significant amount time. contrast, LFAs utilize nanomaterial-based optical sensing technologies, including colorimetric, fluorescence, surface-enhanced Raman scattering (SERS), offering quick, straightforward analyses with minimal training infrastructure requirements detecting proteins biological samples. This review describes composition mechanism recent protein detection, categorizing them into fluorescent, SERS-based techniques. progress, developing simple, stable, highly sensitive, selective LFA system remains formidable challenge. Nevertheless, an advanced promises not only enhance clinical diagnostics but also extend its utility environmental monitoring beyond, demonstrating potential revolutionize both healthcare safety.

Язык: Английский

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Peptide nucleic acid probe-assisted paper-based electrochemical biosensor for multiplexed detection of respiratory viruses

Talanta, Год журнала: 2024, Номер 279, С. 126613 - 126613

Опубликована: Июль 24, 2024

Язык: Английский

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Recent advances on nanomaterials for personalized diagnostics in multiplex point-of-care testing

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 176, С. 117761 - 117761

Опубликована: Май 14, 2024

Язык: Английский

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Polymer Microspheres Copolymerized with Deep Red Fluorescent Molecules as a Label for Lateral Flow Immunochromatography

Langmuir, Год журнала: 2024, Номер 40(13), С. 6971 - 6979

Опубликована: Март 22, 2024

The development of fluorescently labeled microspheres is a critical aspect advancing the technology lateral flow immunochromatography (LFIA) for biological detection. Nevertheless, potential interference posed by background fluorescence originating from nitrocellulose (NC) membrane would significantly impact sensitivity and accuracy microsphere-based detection in LFIA. In this work, an attempt was made to extend π-conjugated system asymmetric structure rhodamine fluorophore, resulting synthesis dye molecules (RB2) incorporating double bonds, which can reach absolute photoluminescence quantum yield (PLQY) 30.01% EtOH. Subsequently, carboxyl group functionalized fluorescent were prepared two-step copolymerization via soap-free emulsion polymerization. obtained characterized scanning electron microscopy, transmission DLS, Fourier transform infrared spectroscopy, ultraviolet spectrophotometry, spectrophotometry. results showed that RB2 successfully copolymerized into microspheres, had good dispersion stability with high red intensity (λabs ∼ 610 nm, λem 660 nm). Utilizing these immunoassay found detect SARS-CoV-2 N protein limit 2.5 pg/mL linear concentration spanning 10 ng/mL. confirm effectiveness synthetic as label

Язык: Английский

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Simple and Ultrasensitive Nanozyme-Linked Immunosorbent Assay for SARS-CoV-2 Detection on a Syringe-Driven Filtration Device

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(34), С. 44485 - 44492

Опубликована: Авг. 16, 2024

This work proposed a simple and ultrasensitive nanozyme-based immunoassay on filtration device for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (NP). Gold core porous platinum shell nanoparticles (Au@Pt NPs) were synthesized with high catalytic activity to oxidize 3,3',5,5'-tetramethylbenzidine, leading an oblivious color change. The was designed based size difference magnetic beads, filter membrane pore, Au@Pt NPs. A simple, rapid, consistent washing procedure can be performed help plastic syringe. method could realize quantitative SARS-CoV-2 NP within 80 min point-of-care needs. limit antigen 0.01 ng/mL in buffer. coefficients variation assay 1.78% 10 antigen, 2.03% 1 2.34% negative sample, respectively. specificity platform verified by various viruses. effective system expected promote substantial progress development application virus immunodetection technology.

Язык: Английский

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