Development of an Integrated Sample Amplification Control for Salivary Point-of-Care Pathogen Testing DOI Creative Commons
Navaporn Sritong,

Winston Wei Ngo,

Karin F.K. Ejendal

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 3, 2023

The COVID-19 pandemic has led to a rise in point-of-care (POC) and home-based tests, but concerns over usability, accuracy, effectiveness have arisen. incorporation of internal amplification controls (IACs), essential control for translational POC diagnostics, could mitigate false-negative false-positive results due sample matrix interference or inhibition. Although emerging nucleic acid tests (NAATs) detecting SARS-CoV-2 show impressive analytical sensitivity the lab, assessment clinical accuracy with IACs is often overlooked. In some cases, were run spatially, complicating assay workflow. Therefore, multiplex pathogen IAC needed.

Язык: Английский

Sensitive detection of K-ras gene by a dual-mode “on-off-on” sensor based on bipyridine ruthenium-MOF and bis-enzymatic cleavage technology DOI
Haotian Xie, Zhaojiang Yin,

Guobin Wei

и другие.

Bioelectrochemistry, Год журнала: 2024, Номер 161, С. 108845 - 108845

Опубликована: Ноя. 5, 2024

Язык: Английский

Процитировано

1
Multiplexed Reverse Transcription Loop-Mediated Isothermal Amplification Coupled with a Nucleic Acid-Based Lateral Flow Dipstick as a Rapid Diagnostic Method to Detect SARS-CoV-2 DOI Creative Commons
D Simon, Chee-Wei Yew, Vijay Kumar Subbiah

и другие.

Microorganisms, Год журнала: 2023, Номер 11(5), С. 1233 - 1233

Опубликована: Май 7, 2023

Due to the high reproduction rate of COVID-19, it is important identify and isolate infected patients at early stages infection. The limitations current diagnostic methods are speed, cost, accuracy. Furthermore, new viral variants have emerged with higher rates infectivity mortality, many mutations various primer binding sites, which may evade detection via conventional PCR kits. Therefore, a rapid method that sensitive, specific, cost-effective needed for point-of-care molecular test. Accordingly, we developed SARS-CoV-2 kit specificity sensitivity, RT-PCR, taking advantage loop-mediated isothermal amplification (LAMP) technique. Four sets six primers were designed based on conserved regions genome: two outer, inner loop primers. Using optimized protocol, genes detected as quickly 10 min but most sensitive 30 min, detecting little 100 copies template DNA. We then coupled RT-LAMP lateral flow dipstick (LFD) multiplex detection. LFD could detect genic amplifications single strip, making suitable multiplexed development RT-LAMP-LFD reaction crude VTM samples would be diagnosis COVID-19 in laboratories well private homes.

Язык: Английский

Процитировано

2
Recent developments in isothermal amplification technology for rapid detection of SARS-CoV-2 DOI
Linlin Wang,

Ruizhe Tang,

Wentao Wang

и другие.

Analytical Methods, Год журнала: 2024, Номер unknown

Опубликована: Дек. 16, 2024

The latest achievements and prospects of LAMP RPA in the rapid detection common infectious disease pathogens such as SARS-CoV-2 is summarized.

Язык: Английский

Процитировано

0
Development of an integrated sample amplification control for salivary point-of-care pathogen testing DOI
Navaporn Sritong,

Winston Wei Ngo,

Karin F.K. Ejendal

и другие.

Analytica Chimica Acta, Год журнала: 2023, Номер 1287, С. 342072 - 342072

Опубликована: Ноя. 29, 2023

Язык: Английский

Процитировано

1
Development of Loop-Mediated Isothermal Amplification Method for Rapid and Sensitive Identification of Hermetia illucens (Diptera: Stratiomyidae) DOI Creative Commons
Wenchao Zhu, Mubasher Hussain, Jing Gao

и другие.

Methods and Protocols, Год журнала: 2023, Номер 6(5), С. 81 - 81

Опубликована: Сен. 5, 2023

The black soldier fly (BSF) is well known for its ability to biologically convert organic waste into insect biomass, including protein and oil, which can be utilised as animal feed. Since raw BSF products, such powder, are difficult differentiate from other biological materials, therefore new analytical approaches required. In this study, we have developed a fast method based on loop-mediated isothermal AMPlification (LAMP) reaction that diagnose larvae byproducts with high accuracy, specificity sensitivity. Species-specific primers were designed targeting the mitochondrial cytochrome C oxidase I (COI) gene. assay was able detect low 820 fg/L of DNA in 60 min at 65 °C, hundredfold higher than detection limit classical polymerase chain did not show cross-reactivity. conclusion, LAMP demonstrated excellent sensitivity byproducts, sampling-to-result identification time min.

Язык: Английский

Процитировано

0
Development of an Integrated Sample Amplification Control for Salivary Point-of-Care Pathogen Testing DOI Creative Commons
Navaporn Sritong,

Winston Wei Ngo,

Karin F.K. Ejendal

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 3, 2023

The COVID-19 pandemic has led to a rise in point-of-care (POC) and home-based tests, but concerns over usability, accuracy, effectiveness have arisen. incorporation of internal amplification controls (IACs), essential control for translational POC diagnostics, could mitigate false-negative false-positive results due sample matrix interference or inhibition. Although emerging nucleic acid tests (NAATs) detecting SARS-CoV-2 show impressive analytical sensitivity the lab, assessment clinical accuracy with IACs is often overlooked. In some cases, were run spatially, complicating assay workflow. Therefore, multiplex pathogen IAC needed.

Язык: Английский

Процитировано

0