Applied Organometallic Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 18, 2024
ABSTRACT
Herein,
two
deep
red
phosphorescent
responsive
emissive
cyclometalated
iridium
(III)
complexes
with
CAIX
inhibition
moiety
was
designed
and
synthesized;
they
can
penetrate
into
living
cells
quickly
mainly
located
in
the
lysosome.
They
showed
a
strong
binding
affinity
towards
vitro
effectively
reduced
cellular
expression
of
CAIX.
Moreover,
tethered
CAIX‐inhibition
benzene
sulfonamide
decrease
inherent
cytotoxicity
Ir(III)
complex
normal
improve
killing
selectivity
cancer
dark;
Ir3
Ir4
exhibit
approximately
five
to
eight
times
higher
than
that
cisplatin;
satisfied
photodynamic
therapy
effect
under
irradiation
425
nm,
ultimately
resulting
apoptosis
cell,
which
is
accompanied
weakening
extracellular
acidification,
increased
expression,
significant
loss
mitochondrial
membrane
potential,
elevated
level
reactive
oxygen.
our
work
may
demonstrate
combination
metal
photosensitizer
provide
promising
strategy
for
constructing
novel
theranostic
platform.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 16, 2025
Cancer
is
a
global
health
challenge
that
urgently
requires
more
sensitive
and
effective
cancer
detection
methods.
Fluorescence
imaging
with
small
molecule
fluorescent
probes
has
shown
great
promise
for
but
most
of
the
developed
lack
active
tumor
cell
targeting,
which
makes
them
unable
to
selectively
target
tumors,
thereby
reducing
accuracy
in
vivo
detection.
Herein,
we
report
novel
probe
Bio-S
combines
viscosity-sensitive
membrane
targetable
group
biotin
targeted
precise
visualization
cells
tumors.
exhibits
fluorescence
changes
viscosity
at
∼660
nm
excellent
localization
ability
(red
fluorescence,
wash-free,
long-term
imaging).
Moreover,
compared
nonbiotinylated
control
C6-S,
biotinylated
can
specifically
membranes,
achieving
much
higher
selectivity
sensitivity
distinguishing
from
normal
cells.
Mice
experiments
show
tail
vein
injection
tumors
monitor
lung
metastasis
level.
Therefore,
this
work
provides
an
new
strategy
tool
tumor-targeted
diagnosis.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 31, 2025
Revealing
changes
in
the
tumor
microenvironment
is
crucial
for
understanding
cancer
and
developing
sensitive
methods
precise
imaging
diagnosis.
Intracellular
hydrogen
peroxide
(H2O2)
microenvironmental
factors
(e.g.,
viscosity
polarity)
are
closely
linked
to
various
physiological
pathological
processes,
making
them
potential
biomarkers
cancer.
However,
a
triple-response
theranostic
probe
therapy
has
not
yet
been
achieved
due
lack
of
effective
tools.
Herein,
we
present
mitochondria-targeting
near-infrared
(NIR)
fluorescent
probe,
VPH-5DF,
capable
simultaneously
monitoring
H2O2,
viscosity,
polarity
through
dual
NIR
channels.
The
specifically
detects
H2O2
via
emission
(λem
=
650
nm)
shows
high
sensitivity
viscosity/polarity
deep
channel
≈
750
nm).
Furthermore,
only
monitors
mitochondrial
polarity,
fluctuations
endogenous/exogenous
levels
but
also
distinguishes
cells
from
normal
multiple
parameters.
Additionally,
VPH-5DF
can
be
employed
monitor
alterations
levels,
as
well
during
drug-induced
pyroptosis
living
cells.
After
treatment
with
chemotherapy-induced
oxidative
damage
mitochondria
activated
pathway,
leading
robust
antitumor
response,
evidenced
xenograft
models.
Thus,
this
prodrug
offers
new
platform
vivo
diagnosis
anticancer
chemotherapy.
