Rhanterium Epapposum Essential Oil and Its Primary Compounds Control Infection, Inflammation, and Serum Electrolyte Imbalance in Mice with Giardiasis
Acta Parasitologica,
Год журнала:
2025,
Номер
70(1)
Опубликована: Янв. 24, 2025
Язык: Английский
Potential activity of Ferula macrecolea essential oil for treating Giardia lamblia infection through modulating electrolytes and suppressing NF-κB p65 pathway
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 18, 2025
The
pharmacological
treatment
of
Giardia
lamblia
infection
involves
the
use
chemical
agents,
such
as
metronidazole
(MNZ).
However,
these
medications
are
associated
with
a
range
adverse
effects,
and
their
effectiveness
is
not
definitively
established.
In
light
previously
discussed
information
recognized
antimicrobial
properties
Ferula
macrecolea,
this
study
aims
to
investigate
both
in
vitro
vivo
anti-giardial
effects
F.
macrecolea
essential
oil
(FME)
on
G.
infection.
Gas
chromatography-mass
spectrometry
(GC-MS)
was
utilized
analyze
composition
prepared
FME.
MTT
colorimetric
assay
employed
assess
FME's
cytotoxic
activities.
were
evaluated
compared
MNZ
mice
infected
lamblia.
Additionally,
FME
therapy
serum
electrolyte
levels
expression
inflammatory
cytokines
assessed.
primary
components
identified
terpinolene
(78.72%),
n-nonanal
(4.47%),
linalool
(4.35%).
significantly
reduced
viability
growth
rate
trophozoites
(IC50
=
21.6
μg/mL)
cysts
27.6
dose-dependent
manner
control
group
(p
<
0.001).
CC50
value
for
against
normal
intestinal
cells
determined
be
207.4
μg/mL.
vivo,
assays
demonstrated
that
administration
various
doses
FME,
particularly
combination
over
7
days,
resulted
statistically
significant
reduction
mean
number
cysts,
level
electrolytes
(sodium
potassium),
interleukin-1
(IL-1),
tumor
necrosis
factor-alpha
(TNF-α),
nuclear
factor
κB
p65
(NF-κB
p65),
Toll-like
receptor
4
(TLR-4)
giardiasis
This
study's
results
demonstrate
extract's
efficacy
lamblia,
exhibiting
minimal
cytotoxicity
towards
cells.
Furthermore,
extract
shown
manage
murine
models
by
modulating
responses
via
suppressing
NF-κB
p65/TLR
pathways.
further
research
necessary
clarify
specific
mechanisms
action
combating
Язык: Английский
Correlation between Melatonin and Colostral Regulatory T Cells in Giardia lamblia Infection
Biomolecules,
Год журнала:
2024,
Номер
14(7), С. 744 - 744
Опубликована: Июнь 24, 2024
Giardiasis
is
a
parasitic
disease
caused
by
Giardia
lamblia
(G.
lamblia)
that
affects
people
worldwide.
Still,
few
studies
report
on
the
immunoregulatory
effects
of
biomolecules
colostrum
during
interactions
with
G.
lamblia.
This
study
aimed
to
assess
concentrations
melatonin
and
cortisol
hormones,
percentage
Treg
cells,
levels
cytokines
IL-10
TGF-β
in
from
mothers
who
tested
positive
for
parasite.
cross-sectional
analyzed
samples
25
puerperal.
The
were
using
an
ELISA
determine
if
they
seropositive
type
antibody
present
(IgM
IgG).
Based
results,
divided
into
three
groups:
control
group
(N
=
10)
no
reaction
either
IgM
or
IgG,
IgG
(IgG+/IgM−;
N
8),
(IgM+/IgG−;
7).
measured
method.
Additionally,
immunophenotyping
flow
cytometry.
In
anti-G.
lamblia,
concentration
was
lower.
However,
level
this
hormone
had
increased.
similar
between
groups,
regardless
seropositivity.
There
higher
cells
also
increased
seronegative
there
correlation
cells.
These
data
suggest
increase
regulatory
reduction
recent
giardia
infection
may
contribute
evolution
manifestation
disease.
Язык: Английский