Macromolecular Rapid Communications,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 22, 2024
Abstract
Protein
function
results
from
the
precise
folding
of
polypeptides
into
bespoke
architectures.
Taking
inspiration
nature,
field
single‐chain
nanoparticles
(SCNPs),
intramolecularly
crosslinked
synthetic
polymers,
emerged.
In
contrast
to
SCNPs
is
generally
defined
by
parent
polymer
or
applied
crosslinker,
rather
than
crosslinking
process
itself.
This
work
explores
cyanopyridine–aminothiol
click
reaction
crosslink
peptide‐decorated
polymers
intra‐macromolecularly
endow
resulting
with
emerging
functionality,
conversion
N‐terminal
cysteine
units
pyridine‐thiazolines.
Dimethylacrylamide
based
different
cysteine‐terminated
amino
acid
sequences
tethered
their
sidechains
are
investigated
(
P1
(C),
P2
(GDHC),
P3
(GDSC))
and
SCNPs.
Since
deprotection
yields
disulfide‐based
SCNPs,
a
direct
comparison
between
disulfide
pyridine‐thiazolines
possible.
revealed
two
properties
pyridine‐thiazoline
SCNPs:
1)
The
formation
gave
rise
metal
binding
sites
within
SCNP,
which
complexed
iron.
2)
Depending
on
peptide
sequence
in
precursor
polymer,
hydrolytic
activity
either
increased
(GDHC)
decreased
(GDSC)
upon
compared
identical
crosslinks.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 17, 2025
In
this
work,
a
photoelectrochemical
(PEC)
sensor
for
ovarian
cancer
detection
was
constructed
based
on
TiO2/poly(5-formylindole)
(P5FIn)heterojunction
using
cascade
amplification.
The
combined
of
multiple
markers
instead
single
mode
can
improve
diagnostic
accuracy.
photoelectron
conversion
efficiency
and
photocurrent
response
were
significantly
improved
by
the
reaction
detector
glutathione
(GSH)
with
cystine.
17β-Estradiol
(E2)
had
negative
effect
PEC
response,
reducing
signal,
ultimately
realizing
sensitive
GSH
E2.
good
performance
E2,
linear
range
(LDR)
0.5–1000
μM,
limits
(LOD)
0.167
μM
0.1224
pM,
respectively,
0.0003671–36.71
nM.
Meanwhile,
also
has
analytical
capability,
signal
amplification
strategy
potential
development
prospect
in
disease
detection.
Signal Transduction and Targeted Therapy,
Год журнала:
2024,
Номер
9(1)
Опубликована: Ноя. 14, 2024
Abstract
In
the
last
decade,
messenger
ribonucleic
acid
(mRNA)-based
drugs
have
gained
great
interest
in
both
immunotherapy
and
non-immunogenic
applications.
This
surge
can
be
largely
attributed
to
demonstration
of
distinct
advantages
offered
by
various
mRNA
molecules,
alongside
rapid
advancements
nucleic
delivery
systems.
It
is
noteworthy
that
immunogenicity
presents
a
double-edged
sword.
context
immunotherapy,
extra
supplementation
adjuvant
generally
required
for
induction
robust
immune
responses.
Conversely,
non-immunotherapeutic
scenarios,
activation
unwanted
considering
host
tolerability
high
expression
demand
mRNA-encoded
functional
proteins.
Herein,
mainly
focused
on
linear
non-replicating
mRNA,
we
overview
preclinical
clinical
progress
prospects
medicines
encompassing
vaccines
other
therapeutics.
We
also
highlight
importance
focusing
host-specific
variations,
including
age,
gender,
pathological
condition,
concurrent
medication
individual
patient,
maximized
efficacy
safety
upon
administration.
Furthermore,
deliberate
potential
challenges
may
encounter
realm
disease
treatment,
current
endeavors
improvement,
as
well
application
future
advancements.
Overall,
this
review
aims
present
comprehensive
understanding
mRNA-based
therapies
while
illuminating
prospective
development
drugs.
Incorporating
d-amino
acids
into
peptides
can
influence
the
intermolecular
interactions
of
peptides,
thus
determining
morphology
and
functionality
self-assembled
supramolecular
structures.
Based
on
this,
we
propose
a
modular
chirality
regulation
strategy
designed
four
chiral
by
adjusting
different
functional
modules.
The
control
coassembly
nucleic
virus-like
vesicles
with
controlled
diameters
enzyme-responsiveness.
Compared
homochiral
heterochiral
inversion
in
their
hydrophobic
domain
transformed
more
assemblies
response
to
highly
expressed
enzyme
matrix
metalloproteinase
7
(MMP-7)
cancer
cells
showed
higher
endosomal
membrane
disruption
activity.
Moreover,
exhibit
high
efficiency
selectivity
delivering
siRNA
gene
drugs
inhibiting
cell
growth,
achieving
mortality
rate
95%
cells.
These
results
provide
promising
for
designing
peptide-based
acid
delivery
systems
through
modulation.
Journal of Nanotheranostics,
Год журнала:
2025,
Номер
6(2), С. 10 - 10
Опубликована: Апрель 9, 2025
Nanotheranostics—where
nanoscale
materials
serve
both
diagnostic
and
therapeutic
functions—are
rapidly
transforming
gene
therapy
by
tackling
critical
delivery
challenges.
This
review
explores
the
design
engineering
of
various
nanoparticle
systems
(lipid-based,
polymeric,
inorganic,
hybrid)
to
enhance
stability,
targeting,
endosomal
escape
genetic
payloads.
We
discuss
how
real-time
imaging
capabilities
integrated
into
these
platforms
enable
precise
localization
controlled
release
genes,
improving
treatment
efficacy
while
reducing
off-target
effects.
Key
strategies
overcome
barriers
(such
as
proton
sponge
effect
photothermal
disruption)
achieve
nuclear
are
highlighted,
along
with
recent
advances
in
stimuli-responsive
that
facilitate
spatiotemporal
control
expression.
Clinical
trials
preclinical
studies
demonstrate
expanding
role
nanotheranostics
managing
cancer,
inherited
disorders,
cardiovascular
neurological
diseases.
further
address
regulatory
manufacturing
hurdles
must
be
for
widespread
clinical
adoption
nanoparticle-based
therapies.
By
synthesizing
progress
ongoing
challenges,
this
underscores
transformative
potential
effective,
targeted,
image-guided
delivery.
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 7099 - 7121
Опубликована: Июль 1, 2024
Abstract:
Introduction
of
exogenous
genes
into
target
cells
to
overcome
various
tumor
diseases
caused
by
genetic
defects
or
abnormalities
and
gene
therapy,
a
new
treatment
method,
provides
promising
strategy
for
treatment.
Over
the
past
decade,
therapy
has
made
exciting
progress;
however,
it
still
faces
challenge
low
nucleic
acid
delivery
release
efficiencies.
The
emergence
nonviral
vectors,
primarily
nanodelivery
systems
(NDRS),
resulted
in
historic
breakthrough
application
therapy.
NDRS,
especially
stimulus-responsive
NDRS
that
can
respond
timely
manner
changes
internal
external
microenvironment
(eg,
pH,
high
concentration
glutathione/reactive
oxygen
species,
overexpressed
enzymes,
temperature,
light,
ultrasound,
magnetic
field),
shown
excellent
loading
advantages
precision
efficiency
been
widely
applied.
only
disadvantage
is
poor
transfection
limits
in-depth
clinical
practice,
owing
presence
biological
barriers
body.
Therefore,
this
review
first
introduces
development
history
current
obstacles
faced
delivery,
strategies
these
obstacles,
conventional
then
focuses
on
latest
research
progress
improving
efficiency.
Finally,
future
challenges
prospects
may
face
transformation
are
discussed
provide
references
enhancing
Keywords:
treatment,
efficiency,
microenvironment,
physiological
barrier,
efficacy
improvement
Accounts of Chemical Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 19, 2025
ConspectusEmulating
the
structural
features
or
functions
of
natural
systems
has
been
demonstrated
as
a
state-of-the-art
strategy
to
create
artificial
functional
materials.
Inspired
by
assembly
and
bioactivity
proteins,
self-assembly
peptides
into
nanostructures
represents
promising
approach
for
creating
biomaterials.
Conventional
assembled
peptide
biomaterials
are
typically
formulated
in
solution
delivered
pathological
sites
implementing
theranostic
objectives.
However,
this
translocation
entails
switch
from
formulation
conditions
physiological
environment
raises
concerns
about
material
performance.
In
addition,
precise
efficient
accumulation
administered
at
target
remains
significant
challenge,
leading
potential
biosafety
issues
associated
with
off-target
effects.
These
limitations
significantly
hinder
progress
advanced
To
address
these
concerns,
past
few
years
have
witnessed
development
situ
living
new
endeavor
optimizing
biomaterial
performance
benefiting
advances
stimuli-responsive
reactions
regulating
noncovalent
interactions.
refers
processes
via
sites.
Due
advantages
precisely
forming
well-defined
lesions,
situ-formed
assemblies
integrated
interesting
next-generation
biomedical
agents.Despite
great
developing
agents,
research
area
still
suffers
limited
toolkit
operating
under
complicated
conditions.
Considering
amino
acids
being
incorporated
backbones
modified
units,
an
acid
is
concern.
Therefore,
our
laboratory
intensively
engaged
designing
discovering
noncanonical
(ncAAs)
expand
manipulating
various
biological
Thus
far,
we
synthesized
containing
ncAAs
4-aminoproline,
2-nitroimidazole
alanine,
Se-methionine,
sulfated
tyrosine,
glycosylated
serine,
which
allow
us
develop
acid-responsive,
redox-responsive,
enzyme-responsive
systems.
Based
on
ncAAs,
established
complex
self-sorting
assembly,
self-amplified
dissipative
cells
optimize
peptides.
The
resulting
exhibit
morphological
adaptability
microenvironment,
contributes
overcoming
delivery
barriers
improvement
targeting
accumulation.
utilizing
developed
toolkit,
further
created
supramolecular
PROTACs,
antagonists,
probes
cancer
treatment
diagnosis
highlight
implications
usage.
Account,
summarize
journey
emphasis
mechanism
Eventually,
also
provide
forward
conceiving
prospects
challenges
clinical
translation
situ-formulated
