Biomaterials Science, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Nanocomposite hydrogels offer a promising approach to treating diabetic wounds. Key findings include enhanced mechanical properties, antibacterial effects, and the ability regulate wound microenvironment.
Язык: Английский
Advanced Science, Год журнала: 2025, Номер unknown
Опубликована: Фев. 28, 2025
Abstract Current diagnostics for sepsis‐associated acute kidney injury (SA‐AKI) detect damage only at advanced stages, limiting opportunities timely intervention. A DNA origami‐based nanoplatform is developed the early diagnosis and treatment of SA‐AKI. Modified with a fluorophore (Cy5) quencher (BHQ3), origami remains nonfluorescent under normal conditions. During SA‐AKI, elevated microRNA‐21 triggers strand displacement reaction that restores fluorescence signal, enabling real‐time detection. Additionally, photoacoustic changes BHQ3, driven by different excretion rates nanostructure released strands, enable dual‐mode imaging, enhancing diagnostic accuracy. Therapeutically, scavenges reactive oxygen species and, when conjugated antimicrobial peptide Leucine‐Leucine‐37 (LL‐37), exhibits bactericidal effects. This combination boosts survival 80% in SA‐AKI models. dual‐response integrates precise imaging targeted therapy, offering powerful strategy management advancing applications precision nanomedicine.
Язык: Английский
Процитировано
0
ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(30), С. 38979 - 38988
Опубликована: Июль 19, 2024
Renal ischemia-reperfusion injury (IRI) is a major contributing factor to the development of acute kidney (AKI) and has resulted in considerable morbidity mortality. Persistent inflammatory responses excessive reactive oxygen species (ROS) following IRI can severely delay tissue repair, making it challenging effectively promote regeneration. Herein, we report an approach enhance immunotherapy using interleukin-10 (IL-10) regeneration by loading IL-10 onto rectangular DNA origami nanostructures (rDON). rDON significantly renal accumulation retention time IL-10, enabling polarize type 1 macrophages into 2 macrophages, thereby reducing proinflammatory factors increasing anti-inflammatory factors. In addition, helps mitigate harmful effects ROS during IRI. The administration IL-10-loaded improves function, resulting notable reduction blood urea nitrogen, serum uric acid, creatinine levels. Our study demonstrates that integration cytokines within holds promise as strategic for cytokine patients with AKI other disorders.
Язык: Английский
Процитировано
2
Chemistry - A European Journal, Год журнала: 2024, Номер 30(46)
Опубликована: Июнь 6, 2024
Abstract DNA origami nanostructures (DONs) are able to scavenge reactive oxygen species (ROS) and their scavenging efficiency toward ROS radicals was shown be comparable that of genomic DNA. Herein, we demonstrate DONs highly efficient singlet quenchers outperforming double‐stranded (ds) by several orders magnitude. To this end, a mixture rich in is generated light irradiation the photosensitizer methylene blue its cytotoxic effect on Escherichia coli cells quantified presence absence DONs. found vastly superior dsDNA protecting bacteria from ROS‐induced damage even surpass established scavengers. At concentration 15 nM, about 50 000 times more scavengers than at an equivalent concentration. This attributed dominant role oxygen, which has long diffusion length reacts specifically with guanine. The dense packing available guanines into small volume DON increases overall quenching probability compared linear same number base pairs. thus have great potential alleviate oxidative stress caused diverse therapeutic settings.
Язык: Английский
Процитировано
1
Journal of Proteome Research, Год журнала: 2024, Номер unknown
Опубликована: Сен. 12, 2024
Treating diabetic sepsis (DS) can be challenging because of the persistent infection multiple organs. To address this complicated pathological condition, it is necessary to develop advanced materials and gain a better understanding their roles. In study, we developed two-dimensional planar material with rectangular deoxyribonucleic acid origami nanostructure (termed Rec-DON). Rec-DON was used improve liver renal function in DS mice, as preferentially accumulates these organs, has superior anti-inflammatory activity ability scavenge reactive oxygen species. The role treatment mice investigated via quantitative proteomics. This study revealed that regulate key proteins located primarily cytoplasm mitochondrion, involved protein transport, antigen processing presentation, steroid metabolic process, also bind various restore mice. presented kidney targeting its alleviating multiorgan injury DS.
Язык: Английский
Процитировано
0
Biomaterials Science, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Nanocomposite hydrogels offer a promising approach to treating diabetic wounds. Key findings include enhanced mechanical properties, antibacterial effects, and the ability regulate wound microenvironment.
Язык: Английский
Процитировано
0