Carbohydrate Polymers, Год журнала: 2024, Номер 352, С. 123142 - 123142
Опубликована: Дек. 16, 2024
Язык: Английский
Acta Biomaterialia, Год журнала: 2024, Номер 184, С. 144 - 155
Опубликована: Июль 2, 2024
Язык: Английский
Процитировано
11
Chemical Reviews, Год журнала: 2024, Номер unknown
Опубликована: Дек. 2, 2024
Click chemistry has become one of the most powerful construction tools in field organic chemistry, materials science, and polymer as it offers hassle-free platforms for high-yielding synthesis novel easy functionalization strategies. The absence harsh reaction conditions or complicated workup procedures allowed rapid development biofunctional polymeric materials, such biopolymers, tailor-made surfaces, stimulus-responsive polymers, etc. In this review, we discuss various types click reactions─including azide-alkyne cycloadditions, nucleophilic radical thiol reactions, a range cycloadditions (Diels-Alder, tetrazole, nitrile oxide, etc.), sulfur fluoride exchange (SuFEx) reaction, oxime-hydrazone reactions─and their use formation study polymers. Following that, state-of-the-art biological applications "click"-biofunctionalized including both passive (e.g., biosensing bioimaging) "active" ones that aim to direct changes biosystems, e.g., drug delivery, antiviral action, tissue engineering. conclusion, have outlined future directions existing challenges click-based polymers medicinal clinical applications.
Язык: Английский
Процитировано
9
Materials Today Bio, Год журнала: 2025, Номер 31, С. 101483 - 101483
Опубликована: Янв. 11, 2025
Injuries to the central nervous system (CNS) often lead persistent inflammation and limited regeneration. This study developed a clinically relevant injectable hydrogel derived from decellularized human peripheral nerves, with mechanical properties biomimicking native CNS tissue. Using modified Hudson method, sciatic nerves were decellularized, effectively removing immunogenic cellular debris while retaining extracellular matrix. Two delipidation solvents, dichloromethane: ethanol (2:1 v/v) n-hexane: isopropanol (3:1 v/v), evaluated, former achieving optimal lipid removal better digestion. The resulting solution was crosslinked genipin, forming an (iHPN) that gelled within 12 min at 37 °C exhibited stiffness of approximately 400 Pa. Human astrocytes, microglial cell clone 3 (HMC3), mouse RAW 264.7 macrophages cultured individually iHPN, lipopolysaccharide (LPS) added mimic following injury. Compared LPS-activated cells on tissue culture plates (TCP), astrocytes iHPN maintained quiescent state, as evidenced by reduced GFAP expression IL-1β secretion. HMC3 in displayed anti-inflammatory phenotype, shown increased CD206 decreased CD86/CD68 expression, along higher IL-4 lower TNF-α/IL-1β SH-SY5Y neuroblastoma viability improved neuronal differentiation compared TCP. brain neurons had TCP or collagen hydrogels. Overall, is novel has potential for minimally invasive applications, such carrier drug delivery and/or biomaterial support axonal growth.
Язык: Английский
Процитировано
0
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Янв. 17, 2025
Regeneration of the injured endometrium, particularly functional layer, is crucial for prevention uterine infertility. At present, clinical treatment using sodium hyaluronate hydrogel injection limited by its relatively low fluidity, short-term retention, and insufficient bioactive ingredients, so it necessary to develop an advanced healing-promoting hydrogel. The modulation microenvironment Lactobacillus presents a component that can facilitate regeneration layer. Our study introduces multifunctional Lactobacillus-loaded poly(N-isopropylacrylamide)-grafted bacterial cellulose (BC-g-PN@L) designed with superior injectability in situ stability. 25 °C (room temperature), uniform distribution achieved pressure only 7.90 kPa. 37 (body BC-g-PN@L forms robust three-dimensional nanonetwork, providing space substance exchange channels maintain viability bioactivity. Enhanced hydrophobic isopropyl groups poly(N-isopropylacrylamide) side chains rigid substrates, exhibits prolonged retention properties cavity, persisting over 21 days. These attributes endow versatile pro-healing capacity rat model endometrial injury. promotes development injectable hydrogels both histological repair endometrium.
Язык: Английский
Процитировано
0
European Journal of Obstetrics & Gynecology and Reproductive Biology X, Год журнала: 2025, Номер 25, С. 100367 - 100367
Опубликована: Янв. 22, 2025
Advancements in regenerative medicine have led to the applicability of stem cell technology various diseases. Stem cells that self-renewable abilities may differentiate into several types provide therapeutic potential. Among different cells, adult are considered as safest with remarkable potential for application. In this review, we current available evidence regarding application medicine, especially field obstetrics and gynecology. This scoping review aims map describe research on We performed a systematic search PubMed, Google Scholar, Cochrane Library August 2024 identify articles involving used Deduplicate website filter based keywords met our inclusion exclusion criteria. The results were presented recommendations from Preferred Reporting Items Systematic Reviews Meta-Analyses extension Scoping Reviews. found 42 Some studies clinical studies, whereas majority preclinical studies. categorized understand their human subjects. Adult therapy is candidate treatment pathologies promising therapy, degenerative gynecologic diseases, lead further near future.
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140220 - 140220
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13
Опубликована: Янв. 29, 2025
Severe endometrial injuries may cause thin endometrium and intrauterine adhesion in women which can result uterine factor infertility. Current treatments, including surgical separation of adhesions hormonal regeneration the endometrium, often fail to prevent re-adhesion achieve satisfactory reproductive results. Recently, mesenchymal stem cells (MSCs) have become a promising new treatment for IUA. However, challenges such as cell survival transplantation limit effectiveness MSC therapy. Researchers explored various approaches enhance therapeutic efficiency MSCs. Among these, biomaterials been frequently employed due their biocompatibility, degradability, ability provide conducive environment growth. This review discusses use MSC-based therapies reconstruction summarizes evidence from preclinical clinical studies, highlighting efficacy safety these biomaterials. The also addresses future directions this field, advances biomaterial engineering, currently under investigation, personalized medicine approaches. emphasizes significance therapy provides practical guidance developing materials protocols applications.
Язык: Английский
Процитировано
0
Journal of Functional Biomaterials, Год журнала: 2025, Номер 16(2), С. 52 - 52
Опубликована: Фев. 7, 2025
In recent years, factors such as the postponement of childbearing and relaxation policy have led to an increase in proportion cesarean sections other intrauterine surgeries among pregnant women, further increasing incidence uterine scars. Currently, there is a lack effective clinical treatment methods for this study, suture loaded with gene medicine was designed repair Specifically, non-viral vector Lipo8000 first used form complex solution plasmid TGF-β3. Then, it mixed adsorbed surgical sutures pretreated recombinant human type III collagen (RhCol III). vitro experiments confirmed that RhCol were successfully onto could be released expressed. vivo carried out using rat model simulating The section results showed compared scar group, expression level TGF-β3 III+TGF-β3 group increased by 39%, TGF-β1 decreased 62.8%, fibrosis rate 16.8%, which has positive effect on prevention This study integrates therapeutic into sutures, ensuring can come contact wound site after suturing. Moreover, work synergistically promote wounds.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Biomedicines, Год журнала: 2025, Номер 13(3), С. 586 - 586
Опубликована: Фев. 27, 2025
Mesenchymal stem cells (MSCs) are a source of wide range soluble factors, including different proteins, growth cytokines, chemokines, and DNA RNA molecules, in addition to numerous secondary metabolites byproducts their metabolism. MSC secretome can be formally divided into secretory vesicular parts, both which very important for intercellular communication involved processes such as angiogenesis, proliferation, immunomodulation. Exosomes thought have the same content function MSCs from they derived, but also number advantages over cells, low immunogenicity, unaltered functional activity during freezing thawing, lack tumor formation. In addition, pre-treatment with various inflammatory factors or hypoxia alter secretomes so that it modified more effective treatment. Paracrine secreted by improve survival other cell populations several mechanisms, immunomodulatory (mostly anti-inflammatory) anti-apoptotic partly based on Hsp27 upregulation. Reproductive medicine is one fields this cell-free approach has been extensively researched. This review presents possible applications challenges using treatment infertility. secretions shown beneficial effects models female male infertility resulting toxic damage, endocrine disorders, trauma, infectious agents, autoimmune origin.
Язык: Английский
Процитировано
0